摘要
从小麦徐州211的成熟种子诱导愈伤组织,建立了胚性悬浮细胞系。酶解悬浮细胞获得原生质体,用含0.8%琼脂糖的改良MS培养基进行琼脂糖珠培养,再生细胞分裂,并形成愈伤组织。诱导再生愈伤组织分化,得到了完整的再生植株。原生质体培养两周后,加入降渗培养液可促进克隆的形成。在分化培养基中,低浓度蔗糖可提高植株分化率。高浓度的激动素和玉米素对芽的分化有效并能抑制愈伤化。再生愈伤组织诱导分化时期的早晚影响植株分化频率。
The calli were initiated from the mature seeds of wheat XuZhou 211 and the suspe nsion cultures were established. The plotoplasts isolated from suspension cells were cultured in the modified MS medium solidified with 0.8% agarose. The regenerated cells divided and the calli formed. The whole plants were regenerated from the protoplast-derived calli. The colony formation were promoted when the medium with lower osmotic plessute was added after two weeks' culture. When the plotoplast-derived calli were differentiated, the frequency of regenerated plants was increased with lower concentration of sucrose. Shoots were induced effectively with higher concentration of cytokinins and the calliferous shoots were avoided. The frequency of regenerated plants was affected when the protoplastderived calli were transferred onto the differentiation medium in different periods.
出处
《生物工程学报》
CAS
CSCD
北大核心
1990年第2期116-119,共4页
Chinese Journal of Biotechnology
关键词
小麦
原生质体培养
植株再生
Wheat (Triticum asetivum L.)
piotoplast
plant regeneration
