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细管胸腔闭式引流治疗气胸的临床观察 被引量:22
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作者 许文景 黄冬云 +1 位作者 朱慕云 李允模 《临床肺科杂志》 2010年第5期634-635,共2页
目的通过与其他两种常用气胸治疗方法的比较来观察细管胸腔闭式引流治疗气胸的效果。方法采用胸腔穿刺或粗管闭式引流或细管闭式引流三种不同的治疗方法对气胸患者进行治疗,并观察各自的疗效及副作用。结果胸腔穿刺治疗组有效率为62.5%... 目的通过与其他两种常用气胸治疗方法的比较来观察细管胸腔闭式引流治疗气胸的效果。方法采用胸腔穿刺或粗管闭式引流或细管闭式引流三种不同的治疗方法对气胸患者进行治疗,并观察各自的疗效及副作用。结果胸腔穿刺治疗组有效率为62.5%;细管胸腔闭式引流治疗组有效率为92.5%;粗管胸腔闭式引流治疗组有效率为81.4%。结论细管胸腔闭式引流是一种较好的治疗气胸的方法。 展开更多
关键词 气胸 细管 胸腔闭式引流
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Effects of Cypermethrin on Male Reproductive System in Adult Rats 被引量:13
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作者 LI Yan Fang PAN Chen +2 位作者 HU Jin Xia LI Jing XU Li Chun 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2013年第3期201-208,共8页
Objective To evaluate effects of cypermethrin on the testis histology and testosterone, LH and FSH in adult male Sprague-Dawley rats. Methods The intact adult male rats were randomly divided into five groups and were ... Objective To evaluate effects of cypermethrin on the testis histology and testosterone, LH and FSH in adult male Sprague-Dawley rats. Methods The intact adult male rats were randomly divided into five groups and were treated with cypermethrin at doses of 0, 7.5, 15, 30, or 60 mg/kg per day by oral gavage for 15-days. After the treatments, serum was collected for hormone assays. The testes, epididymides, seminal vesicles, and prostates were excised and weighed. The right testis was frozen for daily sperm production and the left one was processed for histopathology. Results Daily sperm production decreased significantly in 30 and 60 mg/(kg.day) groups. Testicular structure abnormalities included atrophic and distorted seminiferous tubules, deformed and disordered arrangement of germ cells, reduced germ cells, Sertoli cells and Leydig cells, vacuolization and multinucleated formations of spermatids in the cypermethrin-treated rats. Vacuolization was found in Sertoli cells and the deformed nucleus was noted in Leydig cells. Serum testosterone reduced significantly in 30 and 60 mg/(kg.day) groups. Serum FSH increased significantly in 60 mg/(kg.day) group. Conclusion Cypermethrin induces impairments of the seminiferous tubules structure and spermatogenesis in the rats. The damages of the male reproductive system may be attributed to the imbalance of circulating testosterone. 展开更多
关键词 CYPERMETHRIN TESTOSTERONE Seminiferous tubule SPERMATOGENESIS
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Effect of a single dose of malathion on spermatogenesisin mice 被引量:10
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作者 Eduardo Bustos-Obregón Patricio González-Hormazabal 《Asian Journal of Andrology》 SCIE CAS CSCD 2003年第2期105-107,共3页
<abstract>Aim: To observe the acute effect of the organophosphorous insecticide malathion on testicular function in mice. Methods: The effects of a single dose of malathion [240 mg/kg (1/12 LD50)] on plasma acet... <abstract>Aim: To observe the acute effect of the organophosphorous insecticide malathion on testicular function in mice. Methods: The effects of a single dose of malathion [240 mg/kg (1/12 LD50)] on plasma acetylcholinesterase (ACE) activity, spermatozoa (epididymal cauda counts and teratozoospermia), testis and plasma testosterone concentration) were evaluated at day 1,8, 16, 35 and 40 after treatment. Results: The sperm count was decreased significantly 24 h after treatment and teratozoospermia was increased at day 35 and 40. The height of the seminiferous epithelium and the diameter of tubular lumen were decreased at day 8. The percentage of tubular blockade was increased between day 8 and 35. A decrease in testosterone plasma level was observed at day 16 after treatment. Conclusion: Malathion damages male reproduction. The depletion of seminiferous tubules and the increase in teratozoospermia may be a genotoxic damage to the renewing spermatogonia, but the possibility of spermatogenic/ spermiogenic disfunction due to a decrease in the plasma testosterone level can not be ruled out. 展开更多
关键词 testicular toxin MALATHION seminiferous tubule TESTOSTERONE
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海昆肾喜胶囊对慢性肾脏病患者肾小管功能的影响 被引量:12
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作者 陈瑛 《中成药》 CAS CSCD 北大核心 2009年第5期682-685,共4页
目的:探讨海昆肾喜胶囊(褐藻多糖硫酸酯)对慢性肾脏病(CKD)患者肾小管功能的影响。方法:102例CKD2、3期患者随机分为治疗组、对照组;对照组给予CKD常规治疗,治疗组在常规治疗的基础上加用海昆肾喜胶囊。观察两组治疗前及治疗后3个月、6... 目的:探讨海昆肾喜胶囊(褐藻多糖硫酸酯)对慢性肾脏病(CKD)患者肾小管功能的影响。方法:102例CKD2、3期患者随机分为治疗组、对照组;对照组给予CKD常规治疗,治疗组在常规治疗的基础上加用海昆肾喜胶囊。观察两组治疗前及治疗后3个月、6个月的尿渗透压(UOP)、N-乙酰-β-葡萄糖苷酶(NAG)、α1-微球蛋白(α1-MG)的变化。结果:治疗组治疗后3个月NAG较治疗前明显下降(P<0.05),治疗后6个月尿渗透压较治疗前明显上升(P<0.05)、α1-MG较治疗前明显下降(P<0.05),对照组的以上指标较治疗前无明显变化(P>0.05)。结论:海昆肾喜胶囊可以减轻CKD患者肾小管损伤,改善肾小管功能。 展开更多
关键词 海昆肾喜胶囊 慢性肾脏病 肾小管
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Blood-testis barrier and spermatogenesis: lessons From genetically-modified mice 被引量:9
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作者 Xiao-Hua Jiang Ihtisham Bukhari +4 位作者 Wei Zheng Shi Yin Zheng Wang Howard J Cooke Qing-Hua Shi 《Asian Journal of Andrology》 SCIE CAS CSCD 2014年第4期572-580,共9页
The blood-testis barrier (BTB) is found between adjacent Sertoli cells in the testis where it creates a unique microenvironment for the development and maturation of meiotic and postmeiotic germ cells in seminiferou... The blood-testis barrier (BTB) is found between adjacent Sertoli cells in the testis where it creates a unique microenvironment for the development and maturation of meiotic and postmeiotic germ cells in seminiferous tubes. It is a compound proteinous structure, composed of several types of cell junctions including tight junctions (TJs), adhesion junctions and gap junctions (GJs). Some of the junctional proteins function as structural proteins of BTB and some have regulatory roles. The deletion or functional silencing of genes encoding these proteins may disrupt the BTB, which may cause immunological or other damages to meiotic and postmeiotic cells and ultimately lead to spermatogenic arrest and infertility. In this review, we will summarize the findings on the BTB structure and function from genetically-modified mouse models and discuss the future perspectives. 展开更多
关键词 blood-testis barrier genetically-modified mouse seminiferous tubule sertoli cells SPERMATOGENESIS
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基于自激振荡脉冲特性的磨粒流抛光不锈钢细管内壁的仿真与实验研究 被引量:9
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作者 邓乾发 郭晨曦 +3 位作者 袁巨龙 周辉 吕冰海 Duc-Nam Nguyen 《表面技术》 EI CAS CSCD 北大核心 2019年第10期363-371,共9页
目的解决传统抛光方法对细管件或微孔内表面抛光困难的问题。方法提出一种基于自激振荡脉冲特性的磨粒流抛光方法。利用自激振荡腔,使磨粒流产生振荡脉冲,实现对细管件或微孔内壁高效抛光。建立自激振荡脉冲磨粒流流体区域的数学模型,... 目的解决传统抛光方法对细管件或微孔内表面抛光困难的问题。方法提出一种基于自激振荡脉冲特性的磨粒流抛光方法。利用自激振荡腔,使磨粒流产生振荡脉冲,实现对细管件或微孔内壁高效抛光。建立自激振荡脉冲磨粒流流体区域的数学模型,并通过数值仿真计算获得自激振荡腔体的结构参数d2/d1和L/D以及过渡角β。搭建实验平台,实验验证了自激振荡脉冲特性磨粒流抛光方法的有效性。结果不锈钢细管件的抛光结果表明,加工12 h后,不锈钢细管件内壁的粗糙度Ra从480 nm降到50 nm,内壁面轮廓无明显的单向性纹理;14 h后,不锈钢细管内壁有明显镜面效果。而无振荡腔的情况下需要磨粒流抛光14 h,管件内壁表面粗糙度才达到55 nm,壁面轮廓存在明显的磨粒流抛光流动方向的纹路。结论通过仿真和实验证明了自激振荡脉冲效应抛光方法(SOAFP)的有效性。此外,在本实验条件下,自激振荡腔体的结构参数d2/d1=1.6、L/D=0.5和过渡角β=60°时,抛光效率和抛光后表面质量最佳。 展开更多
关键词 细管 内表面 自激振荡脉冲特性 磨粒流 抛光 不锈钢细管内壁
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大黄抑制近端小管和髓袢升支粗段小管Na^+—K^+ATP酶活性 被引量:6
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作者 郑丰 黄宁昌 +1 位作者 钱斌 黎磊石 《肾脏病与透析肾移植杂志》 CAS CSCD 1995年第4期307-312,共6页
在体内和体外实验中系统地观察了中药大黄对大鼠近端小管和髓袢升支粗段小管Na ̄+-k ̄+ATP酶活性的影响,发现大黄对此二节段肾小管Na ̄+-K ̄+ATP酶均具有显著抑制作用,尤其是对髓袢升支粗段作用更为明显。大黄的作... 在体内和体外实验中系统地观察了中药大黄对大鼠近端小管和髓袢升支粗段小管Na ̄+-k ̄+ATP酶活性的影响,发现大黄对此二节段肾小管Na ̄+-K ̄+ATP酶均具有显著抑制作用,尤其是对髓袢升支粗段作用更为明显。大黄的作用随剂量增大而增强,50mg/L大黄对髓袢升支粗段Na ̄+K ̄+ATP酶活性的抑制率达42.9%。体内应用大黄后,出现利钠、利尿效应。本实验结果进一步明确了大黄对残余肾肾小管高代谢的抑制作用。 展开更多
关键词 肾小管 大黄 腺苷三磷酸 中药 药理
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MFI沸石前驱体和硅酸钠共组装制备水热稳定的管状介孔分子筛 被引量:5
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作者 郑均林 张晔 +1 位作者 吴东 孙予罕 《化学学报》 SCIE CAS CSCD 北大核心 2004年第14期1357-1361,J006,共6页
在CTAB模板剂的作用下 ,硅酸钠、铝酸钠和含有MFI纳米簇的沸石前躯体溶液通过S+ I-路线共组装得到了高水热稳定的管状形貌介孔分子筛 .XRD结果显示样品具有类似MCM 41的规则排列六方孔道结构 .在沸水中处理 15 0h后 ,介孔特征仍然保留 ... 在CTAB模板剂的作用下 ,硅酸钠、铝酸钠和含有MFI纳米簇的沸石前躯体溶液通过S+ I-路线共组装得到了高水热稳定的管状形貌介孔分子筛 .XRD结果显示样品具有类似MCM 41的规则排列六方孔道结构 .在沸水中处理 15 0h后 ,介孔特征仍然保留 ,显示出很好的水热稳定性 .综合SEM和TEM的观察结果 ,此介孔分子筛具有中空的管状形貌 ,平均管长2 .0 μm ,平均直径为 0 .3 0 μm .含有MFI纳米簇的沸石前躯体作为硅源的一部分加入体系中 ,MFI纳米簇伴随来自于硅酸钠的可溶性硅物种共同进入分子筛骨架 ,在提高水热稳定性的同时 ,没有降低六方纳米孔道的规整度 ,也没有对分子筛形貌调变带来不利影响 .MFI纳米簇中 ,硅氧四面体和铝氧四面体的紧密联结方式在进入分子筛骨架时得以保留是水热稳定性提高的主要原因 .作为硅源的另一部分加入的硅酸钠 ,使合成体系pH值足够高 (pH >13 ) ,加入H2 SO4调节体系pH值到10 ,管状形貌的介孔分子筛在逐步中和过程中形成 .通过这种特殊的共组装方法合成介孔分子筛 ,在获得中空的管状形貌的同时显著提高水热稳定性 ,为这种管状形貌介孔分子筛的应用奠定了基础 . 展开更多
关键词 沸石前驱体 硅酸钠 共组装 制备 水热稳定性 管状形貌 介孔分子筛 硅酸钠 铝酸钠 MFI纳米簇
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氧化三甲胺通过促进成年小鼠心肌细胞T小管重构加重心力衰竭 被引量:8
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作者 靳步 纪方方 +5 位作者 左安俊 刘荟婷 祁琳 何韵 王庆尧 赵鹏 《中国病理生理杂志》 CAS CSCD 北大核心 2020年第6期1034-1041,共8页
目的:探讨氧化三甲胺(trimethylamine N-oxide,TMAO)对体外培养的成年小鼠心肌细胞T小管的影响。方法:通过共聚焦T小管以及钙离子成像,分析小鼠心肌细胞T小管结构的规整度以及钙离子释放与回收障碍;Western blot法和免疫荧光法检测心肌... 目的:探讨氧化三甲胺(trimethylamine N-oxide,TMAO)对体外培养的成年小鼠心肌细胞T小管的影响。方法:通过共聚焦T小管以及钙离子成像,分析小鼠心肌细胞T小管结构的规整度以及钙离子释放与回收障碍;Western blot法和免疫荧光法检测心肌细胞微管蛋白的致密化和聚合;通过超声心动图检测TMAO喂养与常规饲料喂养的小鼠心功能。结果:(1)与对照组相比,经TMAO培养的小鼠心肌细胞T小管密度和T小管功率显著降低(P<0. 01);(2)与对照组相比,TMAO处理组小鼠心肌细胞Ca2+瞬变平均振幅降低(P<0. 01),到达Ca2+瞬变峰值的时间延长,失同步化指数增大(P<0. 01);(3)与对照组相比,TMAO处理组小鼠心肌细胞亲联蛋白2(junctophilin-2,JPH2)功率降低(P<0. 01),在心肌细胞边缘可见明显的JPH2堆积(P<0. 01);(4)与对照组相比,TMAO处理组微管密度显著增高(P<0. 01),微管聚集性增强(P<0. 01);(5)与正常饲料喂养的对照组小鼠相比,TMAO喂养组的小鼠心脏收缩功能和左心室射血分数显著下降(P<0. 05)。结论:TMAO通过促进微管蛋白聚合、JPH2易位和T小管重构而损害了小鼠心功能,导致了心力衰竭的进展。 展开更多
关键词 氧化三甲胺 心力衰竭 T小管 微管蛋白 亲联蛋白2
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Cistanche deserticola decoction alleviates the testicular toxicity induced by hydroxyurea in male mice 被引量:5
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作者 Li Gu Wen-Ting Xiong Chao Wang Hong-Xia Sun Guo-Fu Li Xin Liu 《Asian Journal of Andrology》 SCIE CAS CSCD 2013年第6期838-840,I0011,共4页
This study aimed to evaluate testicular toxicity induced by hydroxyurea (HU) and the possible counteracting effect of an aqueous extract of Cistanche deserticola (CD). HU is an antineoplastic drug that has potenti... This study aimed to evaluate testicular toxicity induced by hydroxyurea (HU) and the possible counteracting effect of an aqueous extract of Cistanche deserticola (CD). HU is an antineoplastic drug that has potential reproductive toxicity, and Herba Cistanche has been used as a tonic for the reproductive system for thousands of years. Sixty mice were randomly divided into five groups. Except mice in normal group, the rest received HU (400 mg kg^-1 body weight) intragastrically. Meanwhile, mice in normal and HU control groups received purified water, and the rest received intragastrically three doses of CD decoctions (1.5, 3.0 and 6.0 g crude drug kg^-1 body weight, respectively) daily for 4 weeks. Severe testes lesions were observed, testes weight (P〈0.01) and serum luteinising hormone levels (P〈0.0 1) were also decreased significantly, in the HU groups. Three doses of CD decoctions alleviated the spermatogenetic cell degeneration induced by HU and modulated the serum sex hormones levels to some extent. 展开更多
关键词 Cistanche deserticola HORMONES HYDROXYUREA semin iferous tubule
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EFFECT OF DENTIN TUBULES TO THE MECHANICAL PROPERTIES OF DENTIN.PART Ⅱ:EXPERIMENTAL STUDY 被引量:3
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作者 霍波 郑泉水 +1 位作者 张清 王嘉德 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2000年第1期75-82,共8页
To verify the theoretical models of varying transversely isotropic stress-strain relations of dentin established in the preceding work(Part Ⅰ),we per- form a set of experiments.Because of the very fine tooth size,it ... To verify the theoretical models of varying transversely isotropic stress-strain relations of dentin established in the preceding work(Part Ⅰ),we per- form a set of experiments.Because of the very fine tooth size,it usually seems to be difficult to directly measure the inhomogeneous and anisotropic parameters of dentin.In this paper,by the digital speckle correlation method,tensile experiments are made on the small dentin samples either parallel or perpendicular to the dentin tubules.With the theoretically predicted elastic stress-strain relations,an optimiza- tion method is proposed to fit the strain curve adapted to the experimental data. The results show that the theoretical elastic stress-strain relations coincides very well with the experimental observations.The determined Young's modulus and Poisson's ratio of dentin matrix are 29.5GPa and 0.44,respectively,in the optimization sense. 展开更多
关键词 DENTIN dentin tubule ANISOTROPY stress-strain relations EXPERIMENTS digital speckle correlation
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Bioinspired mineral-in-shell nanoarchitectonics:Functional empowerment of mineral precursors for guiding intradentinal mineralization
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作者 Xiaoran Zheng Yang Liu +10 位作者 Mingjing Li Yuyan Li Wanshan Gao Rongmin Qiu Jiaqi Xing Jiaojiao Yang Yantao Chen Xinyuan Xu Mingming Ding Jun Luo Jianshu Li 《Nano Research》 SCIE EI CSCD 2024年第5期4338-4349,共12页
Effective mineralization of biological structures poses a significant challenge in hard tissue engineering as it necessitates overcoming geometric complexities and multistep biomineralization processes.In this regard,... Effective mineralization of biological structures poses a significant challenge in hard tissue engineering as it necessitates overcoming geometric complexities and multistep biomineralization processes.In this regard,we propose“mineral-in-shell nanoarchitectonics”,inspired by the nanostructure of matrix vesicles,which can influence multiple mineralization pathways.Our nanostructural design empowers mineral precursors with tailorable properties through encapsulating amorphous calcium phosphate within a multifunctional tannic acid(TA)and silk fibroin(SF)nanoshell.The bioinspired nanosystem facilitates efficient recruitment of mineral precursors throughout the dentin structures,followed by large-scale intradentinal mineralization both in vitro and in vivo,which provides persistent protection against external stimuli.Theoretical simulations combined with experimental studies attribute the success of intradentinal mineralization to the TA-SF nanoshell,which exhibits a strong affinity for the dentin structure,stabilizing amorphous precursors and thereby facilitating concomitant mineral formation.Overall,this bioinspired mineral-in-shell nanoarchitectonics shows a promising prospect for hard tissue repair and serves as a blueprint for next-generation biomineralization-associated materials. 展开更多
关键词 mineral-in-shell NANOHYBRIDS self-assembly BIOMINERALIZATION dentinal tubule occlusion dental hypersensitivity
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Single-cell RNA sequencing reveals the transcriptomic landscape of kidneys in patients with ischemic acute kidney injury 被引量:2
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作者 Rong Tang Peng Jin +12 位作者 Chanjuan Shen Wei Lin Leilin Yu Xueling Hu Ting Meng Linlin Zhang Ling Peng Xiangcheng Xiao Peter Eggenhuizen Joshua DOoi Xueqin Wu Xiang Ding Yong Zhong 《Chinese Medical Journal》 SCIE CAS CSCD 2023年第10期1177-1187,共11页
Background:Ischemic acute kidney injury(AKI)is a common syndrome associated with considerable mortality and healthcare costs.Up to now,the underlying pathogenesis of ischemic AKI remains incompletely understood,and sp... Background:Ischemic acute kidney injury(AKI)is a common syndrome associated with considerable mortality and healthcare costs.Up to now,the underlying pathogenesis of ischemic AKI remains incompletely understood,and specific strategies for early diagnosis and treatment of ischemic AKI are still lacking.Here,this study aimed to define the transcriptomic landscape of AKI patients through single-cell RNA sequencing(scRNA-seq)analysis in kidneys.Methods:In this study,scRNA-seq technology was applied to kidneys from two ischemic AKI patients,and three human public scRNA-seq datasets were collected as controls.Differentially expressed genes(DEGs)and cell clusters of kidneys were determined.Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis,as well as the ligand-receptor interaction between cells,were performed.We also validated several DEGs expression in kidneys from human ischemic AKI and ischemia/reperfusion(I/R)injury induced AKI mice through immunohistochemistry staining.Results:15 distinct cell clusters were determined in kidney from subjects of ischemic AKI and control.The injured proximal tubules(PT)displayed a proapoptotic and proinflammatory phenotype.PT cells of ischemic AKI had up-regulation of novel pro-apoptotic genes including USP47,RASSF4,EBAG9,IER3,SASH1,SEPTIN7,and NUB1,which have not been reported in ischemic AKI previously.Several hub genes were validated in kidneys from human AKI and renal I/R injury mice,respectively.Furthermore,PT highly expressed DEGs enriched in endoplasmic reticulum stress,autophagy,and retinoic acid-inducible gene I(RIG-I)signaling.DEGs overexpressed in other tubular cells were primarily enriched in nucleotide-binding and oligomerization domain(NOD)-like receptor signaling,estrogen signaling,interleukin(IL)-12 signaling,and IL-17 signaling.Overexpressed genes in kidney-resident immune cells including macrophages,natural killer T(NKT)cells,monocytes,and dendritic cells were associated with leukocyte activation,chemotaxis,cell adhesion,a 展开更多
关键词 Ischemic acute kidney injury Single-cell RNA sequencing Proximal tubule cells Apoptosis Inflammation Cell-cell crosstalk
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Posterior gut development in Drosophila:a model system for identifying genes controlling epithelial morphogenesis 被引量:5
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作者 LENGYEL JUDITH A XUE JUN LIU (Department of Molecular, Cell and Developmental Biology University of California at Los Angeles, Los Angeles, CA USA, 90095-1606 USA) 《Cell Research》 SCIE CAS CSCD 1998年第4期273-284,共12页
The posterior gut of the Drosophila embryo, consisting of hindgut and Malpighian tubules, provides a simple,well-defined system where it is possible to use a genetic approach to define components essential for epithel... The posterior gut of the Drosophila embryo, consisting of hindgut and Malpighian tubules, provides a simple,well-defined system where it is possible to use a genetic approach to define components essential for epithelial morphogenesis.We review here the advantages of Drosophila as a model genetic organism, the morphogenesis of the epithelial structures of the posterior gut, and what is known about the genetic requirements to form these structures.In overview, primordia are patterned by expression of hierarchies of transcription factors; this leads to localized expression of cell signaling molecules, and finally, to the least understood step: modulation of cell adhesion and cell shape. We describe approaches to identify additional genes that are required for morphogenesis of these simple epithelia, particularly those that might play a structural role by affecting cell adhesion and cell shape. 展开更多
关键词 ORGANOGENESIS cell rearrangement convergent extension HINDGUT Malpighian tubule
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Dentin-desensitizing biomaterials 被引量:1
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作者 Qihui Wang Jiayi Luan +3 位作者 Zhilong Zhao Weihui Kong Congxiao Zhang Jianxun Ding 《Chinese Chemical Letters》 SCIE CAS CSCD 2023年第8期49-56,共8页
Dentin hypersensitivity(DH)associated with dentinal tubule exposure is one of the most common causes of toothache with a rapid onset and short duration.Medication,filling repair,laser irradiation,crown therapy,and des... Dentin hypersensitivity(DH)associated with dentinal tubule exposure is one of the most common causes of toothache with a rapid onset and short duration.Medication,filling repair,laser irradiation,crown therapy,and desensitizing toothpaste are standard clinical treatment strategies,but unsatisfactory treatment modalities are marked by long-term administration,poor dentinal tubule closure,microleakage,and the development of secondary caries.To improve the treatment efficiency of DH,numerous organic or inorganic biomaterials have been developed to relieve toothache and reverse the instability of desensitization.Biomaterials are expected to participate in dental remineralization to achieve desensitization.This review discusses various biomaterials for DH therapy based on different desensitization mechanisms,including dentinal tubule closure and dental nerve blockade,and presents a perspective on the underlying future of dentin regeneration medicine for DH therapy. 展开更多
关键词 Biomaterial Dentin hypersensitivity Dentinal tubule closure Dental nerve blockade Regenerative medicine
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窒息宫内窘迫对早期新生儿肾功能的影响 被引量:5
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作者 蔡勇 谢宗德 +1 位作者 陈平洋 丁依玲 《中国当代儿科杂志》 CAS CSCD 2006年第3期184-186,共3页
目的该文通过测定尿α1微球蛋白(α1-MG)、β2微球蛋白(β2-MG)、白蛋白(Alb)含量,探讨窒息、宫内窘迫对早期新生儿。肾功能的影响,以期对窒息、宫内窘迫后。肾损伤的临床动态监护和防治提供依据。方法用放射免疫法测定窒息组... 目的该文通过测定尿α1微球蛋白(α1-MG)、β2微球蛋白(β2-MG)、白蛋白(Alb)含量,探讨窒息、宫内窘迫对早期新生儿。肾功能的影响,以期对窒息、宫内窘迫后。肾损伤的临床动态监护和防治提供依据。方法用放射免疫法测定窒息组、宫内窘迫组新生儿生后0~2d,3~4d,6~7d尿α1-MG,β2-MG,Alb含量。结果窒息组3个时段尿α1-MG,β2-MG,Alb均高于正常对照组,以3~4d最高,差异有显著性(P〈0.05)。重度窒息组3个时段尿α1-MG,β2-MG,Alb均高于轻度窒息组,差异有显著性(P〈0.05)。尿α1-MG,β2-MG,Alb在宫内窘迫组3个时段之间比较差异无显著性(P〉0.05)。宫内窘迫组3个时段尿α1-MG,β2-MG,Alb与正常对照组比较差异无显著性(P〉0.05)。结论窒息可导致新生儿肾小球、肾小管功能损伤,3~4d损伤最严重,损伤程度与窒息程度呈正相关。宫内窘迫新生儿生后1周内肾小球、肾小管功能无明显损伤。 展开更多
关键词 肾小球 肾小管 窒息 宫内窘迫 新生儿
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Genetic algorithm-optimized backpropagation neural network establishes a diagnostic prediction model for diabetic nephropathy:Combined machine learning and experimental validation in mice 被引量:1
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作者 WEI LIANG ZONGWEI ZHANG +5 位作者 KEJU YANG HONGTU HU QIANG LUO ANKANG YANG LI CHANG YUANYUAN ZENG 《BIOCELL》 SCIE 2023年第6期1253-1263,共11页
Background:Diabetic nephropathy(DN)is the most common complication of type 2 diabetes mellitus and the main cause of end-stage renal disease worldwide.Diagnostic biomarkers may allow early diagnosis and treatment of D... Background:Diabetic nephropathy(DN)is the most common complication of type 2 diabetes mellitus and the main cause of end-stage renal disease worldwide.Diagnostic biomarkers may allow early diagnosis and treatment of DN to reduce the prevalence and delay the development of DN.Kidney biopsy is the gold standard for diagnosing DN;however,its invasive character is its primary limitation.The machine learning approach provides a non-invasive and specific criterion for diagnosing DN,although traditional machine learning algorithms need to be improved to enhance diagnostic performance.Methods:We applied high-throughput RNA sequencing to obtain the genes related to DN tubular tissues and normal tubular tissues of mice.Then machine learning algorithms,random forest,LASSO logistic regression,and principal component analysis were used to identify key genes(CES1G,CYP4A14,NDUFA4,ABCC4,ACE).Then,the genetic algorithm-optimized backpropagation neural network(GA-BPNN)was used to improve the DN diagnostic model.Results:The AUC value of the GA-BPNN model in the training dataset was 0.83,and the AUC value of the model in the validation dataset was 0.81,while the AUC values of the SVM model in the training dataset and external validation dataset were 0.756 and 0.650,respectively.Thus,this GA-BPNN gave better values than the traditional SVM model.This diagnosis model may aim for personalized diagnosis and treatment of patients with DN.Immunohistochemical staining further confirmed that the tissue and cell expression of NADH dehydrogenase(ubiquinone)1 alpha subcomplex,4-like 2(NDUFA4L2)in tubular tissue in DN mice were decreased.Conclusion:The GA-BPNN model has better accuracy than the traditional SVM model and may provide an effective tool for diagnosing DN. 展开更多
关键词 Diabetic nephropathy Renal tubule Machine learning Diagnostic model Genetic algorithm
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气体粘滞系数的测定 被引量:4
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作者 王云创 李光仲 李德尧 《物理与工程》 2003年第2期37-40,共4页
本文对球形液膜内气体沿细管排出过程中 ,球形液膜的动态特性作了理论模拟推导 。
关键词 气体粘滞系数 测定 球形液膜 动态特性 细管 测量方法 物理实验
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Production of Transgenic Animals Using Spermatogonial Stem Cells 被引量:2
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作者 MIAO Xiang-yang 《Agricultural Sciences in China》 CAS CSCD 2011年第5期762-768,共7页
Spermatogonial stem cells (SSCs) are a type of adult stem cell found in male mammals.These cells have the capacity for self renewal and are capable of differentiating in the niche of testis.They are also the only ad... Spermatogonial stem cells (SSCs) are a type of adult stem cell found in male mammals.These cells have the capacity for self renewal and are capable of differentiating in the niche of testis.They are also the only adult stem cells in a normal postnatal body that undergo self-renewal throughout life,transferring genetic information to the offspring.Since a technique for transplanting SSCs was first described by Brinster and his colleagues in 1994,more and more researchers have become interested in exploring the possibility of utilizing adult SSCs to generate transgenic animals.In this mini-review,we attempt to summarize the current research progress in the area of spermatogonial stem cells including the source,types and differentiation of the SSCs,and the application on transgenic animals,with a particular focus on the strategy of SSCs delivery including seminiferous tubule injection and spermatogonial stem cell transplantation. 展开更多
关键词 spermatogonial stem cells transgenic animal seminiferous tubule injection TRANSPLANTATION
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Angiogenic Factors Are Associated with Development of Acute Graft-Versus-Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation 被引量:2
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作者 聂第敏 吴秋玲 +7 位作者 朱夏夏 张然 郑鹏 方俊 游泳 仲照东 夏凌辉 洪梅 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2015年第5期694-699,共6页
Acute graft-versus-host disease(a GVHD) is a serious complication after allogeneic hematopoietic stem cell transplantation(allo-HSCT). However,the mechanisms of a GVHD are not well understood. We aim to investigat... Acute graft-versus-host disease(a GVHD) is a serious complication after allogeneic hematopoietic stem cell transplantation(allo-HSCT). However,the mechanisms of a GVHD are not well understood. We aim to investigate the roles of the three angiogenic factors: angiopoietin-1(Ang-1),Ang-2 and vascular endothelial growth factor(VEGF) in the development of a GVHD. Twenty-one patients who underwent allo-HSCT were included in our study. The dynamic changes of Ang-1,Ang-2 and VEGF were monitored in patients before and after allo-HSCT. In vitro,endothelial cells(ECs) were treated with TNF-α in the presence or absence of Ang-1,and then the Ang-2 level in the cell culture medium and the tubule formation by ECs were evaluated. After allo-HSCT,Ang-1,Ang-2 and VEGF all exhibited significant variation,suggesting these factors might be involved in the endothelial damage in transplantation. Patients with a GVHD had lower Ang-1 level at day 7 but higher Ang-2 level at day 21 than those without a GVHD,implying that Ang-1 may play a protective role in early phase yet Ang-2 is a promotion factor to a GVHD. In vitro,TNF-α promoted the release of Ang-2 by ECs and impaired tubule formation of ECs,which were both weakened by Ang-1,suggesting that Ang-1 may play a protective role in a GVHD by influencing the secretion of Ang-2,consistent with our in vivo tests. It is concluded that monitoring changes of these factors following allo-HSCT might help to identify patients at a high risk for a GVHD. 展开更多
关键词 GVHD hematopoietic protective allogeneic versus tubule angiopoietin impaired understood secretion
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