The piscidin family, which includes potent antimicrobial peptides with broad-spectrum activity, plays an important role in the innate immune system of fish. In this study, we cloned piscidin-5-1ike type 3 （Lcpis51t3...The piscidin family, which includes potent antimicrobial peptides with broad-spectrum activity, plays an important role in the innate immune system of fish. In this study, we cloned piscidin-5-1ike type 3 （Lcpis51t3） in large yellow croaker （Larimichthys crocea）. Multiple alignments with other known piscidins revealed amino acid conservation throughout the fish, especially at the signal peptide （22 amino acids）. The phylogenetic tree confirmed that Lcpis51t3 and large yellow croaker piscidin-5-1ike proteins were grouped together to form a branch. Quantitative real-time PCR revealed that Lcpis51t3 was expressed in a wide range of tissues, including the brain, muscle, gill, head kidney, intestine, kidney, liver, and spleen. The highest mRNA expression level of Lcpis51t3 was found in the spleen. After Vibrio alginolyticus infection, mRNA expression was rapidly upregulated in the liver, head kidney, gill, kidney, and intestine at 4, 8, 12, and 24 h post infection （hpi）, whereas there were no significant changes in the spleen. The antimicrobial spectrum showed that the synthetic mature peptide of Lcpis51t3 exhibited different activity in vitro against various bacteria, such as Aeromonas hydrophila, V. anguillarum, V. alginolyticus, V. parahaemolyticus, Staphylococcus aureus, and Listeria monocytogenes In addition, survival rates from the in vivo assay indicated that the synthetic peptide of Lcpis51t3 increased the survival rate of large yellow croaker after V. alginolyticus challenge, resulting in a decline in bacterial burden and mRNA expression levels of interleukin-ll3, interleukin-10, and tumor necrosis factor-（]. These data suggest that Lcpis51t3 plays an important role in innate immunity in large yellow croaker and might represent a potential therapeutic agent against pathogen invasion.展开更多
In traditional beekeeping,the two most important methods for extracting honey are centrifugation and honeycomb-pressing.In this study,the physicochemical composition of honey samples extracted using two distinct proce...In traditional beekeeping,the two most important methods for extracting honey are centrifugation and honeycomb-pressing.In this study,the physicochemical composition of honey samples extracted using two distinct procedures was compared,as well as the impact of antioxidant capacity and nutritional potency on the lifespan and learning memory of worker bees.Honey samples were collected from ten colonies of Apis cerana:five samples via centrifugation and five via honeycomb-pressing.Our results showed that honey extraction methods influence the nutri-tional composition and potency of honey.Most parameters were superior in pressed honey,and the amylase activity in centrifuged honey was higher.The effects of antioxidant capacity and nutritional potency on worker bees'lifespans and learning memory were also superior in pressed honey.Pressed honey had higher nutritional composition and potency.However,whether pressed honey,which is rich in pollen,spoils more easilyrequiresfurtherinvestigation.展开更多
基金supported by the National 863 Project(2012AA10A403)the Scientific Research Foundation of Graduate School of Ningbo University(G16089)KC Wong Magna Fund in Ningbo University
文摘The piscidin family, which includes potent antimicrobial peptides with broad-spectrum activity, plays an important role in the innate immune system of fish. In this study, we cloned piscidin-5-1ike type 3 （Lcpis51t3） in large yellow croaker （Larimichthys crocea）. Multiple alignments with other known piscidins revealed amino acid conservation throughout the fish, especially at the signal peptide （22 amino acids）. The phylogenetic tree confirmed that Lcpis51t3 and large yellow croaker piscidin-5-1ike proteins were grouped together to form a branch. Quantitative real-time PCR revealed that Lcpis51t3 was expressed in a wide range of tissues, including the brain, muscle, gill, head kidney, intestine, kidney, liver, and spleen. The highest mRNA expression level of Lcpis51t3 was found in the spleen. After Vibrio alginolyticus infection, mRNA expression was rapidly upregulated in the liver, head kidney, gill, kidney, and intestine at 4, 8, 12, and 24 h post infection （hpi）, whereas there were no significant changes in the spleen. The antimicrobial spectrum showed that the synthetic mature peptide of Lcpis51t3 exhibited different activity in vitro against various bacteria, such as Aeromonas hydrophila, V. anguillarum, V. alginolyticus, V. parahaemolyticus, Staphylococcus aureus, and Listeria monocytogenes In addition, survival rates from the in vivo assay indicated that the synthetic peptide of Lcpis51t3 increased the survival rate of large yellow croaker after V. alginolyticus challenge, resulting in a decline in bacterial burden and mRNA expression levels of interleukin-ll3, interleukin-10, and tumor necrosis factor-（]. These data suggest that Lcpis51t3 plays an important role in innate immunity in large yellow croaker and might represent a potential therapeutic agent against pathogen invasion.
基金supported by the Science and Technology Project of Jiangxi Province(20181ACF60006),China。
文摘In traditional beekeeping,the two most important methods for extracting honey are centrifugation and honeycomb-pressing.In this study,the physicochemical composition of honey samples extracted using two distinct procedures was compared,as well as the impact of antioxidant capacity and nutritional potency on the lifespan and learning memory of worker bees.Honey samples were collected from ten colonies of Apis cerana:five samples via centrifugation and five via honeycomb-pressing.Our results showed that honey extraction methods influence the nutri-tional composition and potency of honey.Most parameters were superior in pressed honey,and the amylase activity in centrifuged honey was higher.The effects of antioxidant capacity and nutritional potency on worker bees'lifespans and learning memory were also superior in pressed honey.Pressed honey had higher nutritional composition and potency.However,whether pressed honey,which is rich in pollen,spoils more easilyrequiresfurtherinvestigation.
