The fact of microcarrier (MC) culture introduces new possibilities and makes possible the practical high-yield culture of anchorage-dependent cells has generated a considerable focus in this study. The objective of th...The fact of microcarrier (MC) culture introduces new possibilities and makes possible the practical high-yield culture of anchorage-dependent cells has generated a considerable focus in this study. The objective of this research was to study the comparison of Vero cell growth on different types of commercial microcarriers;Cytodex-1, Cytodex-3, Hil-lex? II and Plastic Plus in spinner vessel and two liters bioreactor cultured for 96 hours. Biological performance of the microcarrier in RPMI media showed the preference of Vero cell grew on Cytodex 3 microcarriers with highest maximum viable cell number (2.4 × 105 cells/ml) followed by Cytodex 1, Hillex and Plustic Plus. Vero cell on Cyto-dex-3 data in spinner flask was compared in bioreactor and result showed higher viable cell number in biorector. Thus, this dextran-crosslink gelatin microcarrier (Cytodex 3) provided the best surface for cell attachment and fast proliferation. At the end of this cell growth improvement will be used for virus transfection producing a vaccine in bioreactor.展开更多
Introducing the key technique of spinning fine tex yarnson the friction spinner, deals mainly with the technologi-cal parameters of the carding device, conducting tubeand spinning drum for the friction spinning. The e...Introducing the key technique of spinning fine tex yarnson the friction spinner, deals mainly with the technologi-cal parameters of the carding device, conducting tubeand spinning drum for the friction spinning. The experi-mental results show that, the combing effect of pin drumis better than that of garnett; the combing length is a im-portant factor. The form and dimension of the conduct-ing tube influence the rate of fiber straightness and thedegree of fiber orientation. The little angle between theconducting tube and drum axis is good for fiber parallel-ism and straightness. The frictional ratio and the rate ofair flow influence the efficiency of twist.展开更多
A culture system was developed and successfully employed for the serum-free cultivation of Tn-5B1-4 (Tn5 ) insect cells. With our adaptation procedure, it was possible to obtain cells fully adapted to serum-free media...A culture system was developed and successfully employed for the serum-free cultivation of Tn-5B1-4 (Tn5 ) insect cells. With our adaptation procedure, it was possible to obtain cells fully adapted to serum-free media in stationary T-flasks and then enable these adapted cells to grow well in spinner flasks immediately. The spinner "ask with special stirring design proved tO provide favorable culture environment that made it desirable for use in the serum-free cultivation of Tn5 cells even at low seeding density.展开更多
目的比较中性玻璃转瓶和聚苯乙烯细胞工厂两种材质的培养容器培养人二倍体细胞和甲型肝炎病毒(hepatitis A virus,HAV)的差异。方法采用相同的培养条件,在中性玻璃转瓶和聚苯乙烯细胞工厂中分别培养人胚肺二倍体细胞KMB17,并接种HAV H2...目的比较中性玻璃转瓶和聚苯乙烯细胞工厂两种材质的培养容器培养人二倍体细胞和甲型肝炎病毒(hepatitis A virus,HAV)的差异。方法采用相同的培养条件,在中性玻璃转瓶和聚苯乙烯细胞工厂中分别培养人胚肺二倍体细胞KMB17,并接种HAV H2株,倒置光学显微镜下观察细胞的形态变化;细胞接种病毒后第0、7、14、18、22、26、30天时取样,ELISA法测定病毒的感染性滴度,分析病毒的增殖动力学。结果两种材质的容器培养的KMB17细胞的生长状态和形态无明显差异;HAV H2株增殖动力学相似;单位体积病毒收获液中的病毒产量无显著差异;聚苯乙烯细胞工厂培养的HAV的单位培养容积产量是中性玻璃转瓶的7.28倍。结论聚苯乙烯细胞工厂和中性玻璃转瓶培养的人二倍体细胞和HAV均能良好生长,细胞工厂可替代转瓶成为新的疫苗规模化生产的细胞培养技术。展开更多
Environmental impact and economic concerns have driven a variable rate technology(VRT).Spinner spreaders were mainly used for granular fertilizer application since they are simple in design,inexpensive,and can cover l...Environmental impact and economic concerns have driven a variable rate technology(VRT).Spinner spreaders were mainly used for granular fertilizer application since they are simple in design,inexpensive,and can cover large areas.However,the spreader was not adequate for VRT because uniformity changes drastically while varying application rates.Thus,the purpose of this study was to develop a variable rate applicator with uniform spreading patterns.A commercial spreader was modified with a controller and electric actuators for controlling fertilizer discharge directions and amounts.Database was established to determine the optimum discharge direction according to the fertilizer application amount.The uniformity of spreading patterns in accordance with the spread amount per set unit area was evaluated by the statistical coefficient of variation(CV)lower than 15%is assumed to prevent damage to the crop.Test results showed that CVs were 8%,9%,and 8%,respectively,for a tractor in race track mode(adjacent passes in same direction of travel)at 200 kg/hm^(2),300 kg/hm^(2),and 400 kg/hm^(2).This indicates that the fertilizer was spread uniformly,while the coefficient of variation was 12%at 200 kg/hm^(2) in back and forth mode(adjacent passes in the opposite direction of travel).Overall,the results suggest that the race track mode is suitable for operation of a tractor to ensure uniform spreading of fertilizer when applying at variable rates.The future goal is to establish a system for automatic variable rate application according to location in connection with soil analysis and geographic information systems.展开更多
文摘The fact of microcarrier (MC) culture introduces new possibilities and makes possible the practical high-yield culture of anchorage-dependent cells has generated a considerable focus in this study. The objective of this research was to study the comparison of Vero cell growth on different types of commercial microcarriers;Cytodex-1, Cytodex-3, Hil-lex? II and Plastic Plus in spinner vessel and two liters bioreactor cultured for 96 hours. Biological performance of the microcarrier in RPMI media showed the preference of Vero cell grew on Cytodex 3 microcarriers with highest maximum viable cell number (2.4 × 105 cells/ml) followed by Cytodex 1, Hillex and Plustic Plus. Vero cell on Cyto-dex-3 data in spinner flask was compared in bioreactor and result showed higher viable cell number in biorector. Thus, this dextran-crosslink gelatin microcarrier (Cytodex 3) provided the best surface for cell attachment and fast proliferation. At the end of this cell growth improvement will be used for virus transfection producing a vaccine in bioreactor.
文摘Introducing the key technique of spinning fine tex yarnson the friction spinner, deals mainly with the technologi-cal parameters of the carding device, conducting tubeand spinning drum for the friction spinning. The experi-mental results show that, the combing effect of pin drumis better than that of garnett; the combing length is a im-portant factor. The form and dimension of the conduct-ing tube influence the rate of fiber straightness and thedegree of fiber orientation. The little angle between theconducting tube and drum axis is good for fiber parallel-ism and straightness. The frictional ratio and the rate ofair flow influence the efficiency of twist.
文摘A culture system was developed and successfully employed for the serum-free cultivation of Tn-5B1-4 (Tn5 ) insect cells. With our adaptation procedure, it was possible to obtain cells fully adapted to serum-free media in stationary T-flasks and then enable these adapted cells to grow well in spinner flasks immediately. The spinner "ask with special stirring design proved tO provide favorable culture environment that made it desirable for use in the serum-free cultivation of Tn5 cells even at low seeding density.
文摘目的比较中性玻璃转瓶和聚苯乙烯细胞工厂两种材质的培养容器培养人二倍体细胞和甲型肝炎病毒(hepatitis A virus,HAV)的差异。方法采用相同的培养条件,在中性玻璃转瓶和聚苯乙烯细胞工厂中分别培养人胚肺二倍体细胞KMB17,并接种HAV H2株,倒置光学显微镜下观察细胞的形态变化;细胞接种病毒后第0、7、14、18、22、26、30天时取样,ELISA法测定病毒的感染性滴度,分析病毒的增殖动力学。结果两种材质的容器培养的KMB17细胞的生长状态和形态无明显差异;HAV H2株增殖动力学相似;单位体积病毒收获液中的病毒产量无显著差异;聚苯乙烯细胞工厂培养的HAV的单位培养容积产量是中性玻璃转瓶的7.28倍。结论聚苯乙烯细胞工厂和中性玻璃转瓶培养的人二倍体细胞和HAV均能良好生长,细胞工厂可替代转瓶成为新的疫苗规模化生产的细胞培养技术。
基金This work was carried out with the support of the“Cooperative Research Program for Agriculture Science&Technology Development”(Project No.PJ01050303),Rural Development Administration,Republic of Korea.
文摘Environmental impact and economic concerns have driven a variable rate technology(VRT).Spinner spreaders were mainly used for granular fertilizer application since they are simple in design,inexpensive,and can cover large areas.However,the spreader was not adequate for VRT because uniformity changes drastically while varying application rates.Thus,the purpose of this study was to develop a variable rate applicator with uniform spreading patterns.A commercial spreader was modified with a controller and electric actuators for controlling fertilizer discharge directions and amounts.Database was established to determine the optimum discharge direction according to the fertilizer application amount.The uniformity of spreading patterns in accordance with the spread amount per set unit area was evaluated by the statistical coefficient of variation(CV)lower than 15%is assumed to prevent damage to the crop.Test results showed that CVs were 8%,9%,and 8%,respectively,for a tractor in race track mode(adjacent passes in same direction of travel)at 200 kg/hm^(2),300 kg/hm^(2),and 400 kg/hm^(2).This indicates that the fertilizer was spread uniformly,while the coefficient of variation was 12%at 200 kg/hm^(2) in back and forth mode(adjacent passes in the opposite direction of travel).Overall,the results suggest that the race track mode is suitable for operation of a tractor to ensure uniform spreading of fertilizer when applying at variable rates.The future goal is to establish a system for automatic variable rate application according to location in connection with soil analysis and geographic information systems.
