AIM To study the effects of psoralen (PSO) and 8 methoxypsoralen (8 MOP) on the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells. METHODS Inhibitory effects of PSO and 8 MOP on the prolifer...AIM To study the effects of psoralen (PSO) and 8 methoxypsoralen (8 MOP) on the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells. METHODS Inhibitory effects of PSO and 8 MOP on the proliferation and metastatic potential of mucoepidermaid carcinoma Mc3 cells were investigated with MTT assay, cell counting, flow cytometry and tail vein injection of the cells into nude mice (10 6 for each). RESULTS PSO and 8 MOP inhibited Mc3 cell growth in a dose dependent way. The IC 30 (mg·L -1 ) of PSO and 8 MOP were 32.4 and 25.1 and IC 50 (mg·L -1 ) 44.7 and 35.5 respectively. After the cells had been treated with the drugs at IC 30 for 5 d, the doubling time (h) for the control, PSO treated and 8 MOP treated cells were 20.8, 23.3 and 57.8 respectively, the percentage of S phase cells 24.5,17.8 and 5.8, the wild type p53 expression (%) 24.0,99.8 and 99.0, the nm23 H 1 expression (%) 99 9,99.5 and 99.1, the clonogenesity (%) 23.5,16.5 and 0, the number of metastatic foci on lung surface 139±61,114±68 and 36±32, respectively. CONCLUSION Both PSO and 8 MOP at the dosage of IC 30 may inhibit the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells, but 8 MOP is more effective.展开更多
The expression of P16 gene were found in all 3 groups. The positive unit (PU) was higher in tumor group and cancer group than that in normal group ( P <0.01). Furthermore, the PU of P16 was stronger in cytopla...The expression of P16 gene were found in all 3 groups. The positive unit (PU) was higher in tumor group and cancer group than that in normal group ( P <0.01). Furthermore, the PU of P16 was stronger in cytoplasm than in nucleus. Malignant tumors and acini surrounding the tumor revealed strong positives and week positives respectively. The PU of P16 gene was higher in deep lobe of recurrent parotid neoplasm with incomplete capsule than that in shallow lobe of primary parotid neoplasm with complete capsule. Our findings suggests that P16 gene plays equally important role in the salivary gland tumors and tumors in other part of the body.展开更多
Pleomorphic adenoma is the most common benign parotid gland tumor. Although its local recurrence rate is known to be high, the recurrence extending to the cervical region is rare. Here we report a case of a young fema...Pleomorphic adenoma is the most common benign parotid gland tumor. Although its local recurrence rate is known to be high, the recurrence extending to the cervical region is rare. Here we report a case of a young female (25 years old) with pleomorphic adenoma of the parotid gland which showed multiple recurrences through facial to cervical regions over a span of eight years. We also discuss how this benign tumor with a high recurrence rate has been treated in other cases, and how it should be treated.展开更多
This article presents a case of low-grade cribriform cystadenocarcinomas(LGCCC),a rare salivary gland tumor manifesting in the infratemporal fossa(ITF).The lesion in this case is unique in its location,histopathology,...This article presents a case of low-grade cribriform cystadenocarcinomas(LGCCC),a rare salivary gland tumor manifesting in the infratemporal fossa(ITF).The lesion in this case is unique in its location,histopathology,and management in that the tumor resection was performed using an exclusively endoscopic,endonasal approach.This case highlights the expanding application of endoscopic skull base techniques to address an indolent,slow-growing malignancy of the ITF.展开更多
文摘AIM To study the effects of psoralen (PSO) and 8 methoxypsoralen (8 MOP) on the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells. METHODS Inhibitory effects of PSO and 8 MOP on the proliferation and metastatic potential of mucoepidermaid carcinoma Mc3 cells were investigated with MTT assay, cell counting, flow cytometry and tail vein injection of the cells into nude mice (10 6 for each). RESULTS PSO and 8 MOP inhibited Mc3 cell growth in a dose dependent way. The IC 30 (mg·L -1 ) of PSO and 8 MOP were 32.4 and 25.1 and IC 50 (mg·L -1 ) 44.7 and 35.5 respectively. After the cells had been treated with the drugs at IC 30 for 5 d, the doubling time (h) for the control, PSO treated and 8 MOP treated cells were 20.8, 23.3 and 57.8 respectively, the percentage of S phase cells 24.5,17.8 and 5.8, the wild type p53 expression (%) 24.0,99.8 and 99.0, the nm23 H 1 expression (%) 99 9,99.5 and 99.1, the clonogenesity (%) 23.5,16.5 and 0, the number of metastatic foci on lung surface 139±61,114±68 and 36±32, respectively. CONCLUSION Both PSO and 8 MOP at the dosage of IC 30 may inhibit the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells, but 8 MOP is more effective.
文摘The expression of P16 gene were found in all 3 groups. The positive unit (PU) was higher in tumor group and cancer group than that in normal group ( P <0.01). Furthermore, the PU of P16 was stronger in cytoplasm than in nucleus. Malignant tumors and acini surrounding the tumor revealed strong positives and week positives respectively. The PU of P16 gene was higher in deep lobe of recurrent parotid neoplasm with incomplete capsule than that in shallow lobe of primary parotid neoplasm with complete capsule. Our findings suggests that P16 gene plays equally important role in the salivary gland tumors and tumors in other part of the body.
文摘Pleomorphic adenoma is the most common benign parotid gland tumor. Although its local recurrence rate is known to be high, the recurrence extending to the cervical region is rare. Here we report a case of a young female (25 years old) with pleomorphic adenoma of the parotid gland which showed multiple recurrences through facial to cervical regions over a span of eight years. We also discuss how this benign tumor with a high recurrence rate has been treated in other cases, and how it should be treated.
文摘This article presents a case of low-grade cribriform cystadenocarcinomas(LGCCC),a rare salivary gland tumor manifesting in the infratemporal fossa(ITF).The lesion in this case is unique in its location,histopathology,and management in that the tumor resection was performed using an exclusively endoscopic,endonasal approach.This case highlights the expanding application of endoscopic skull base techniques to address an indolent,slow-growing malignancy of the ITF.
