Renal epithelial sodium channel(ENaC) plays a crucial role in maintaining homeostasis and sodium absorption.While insulin participates in controlling sodium transport across the renal epithelium,the underlying molecul...Renal epithelial sodium channel(ENaC) plays a crucial role in maintaining homeostasis and sodium absorption.While insulin participates in controlling sodium transport across the renal epithelium,the underlying molecular mechanism remain unclear.In this study,we found that insulin increased the expression and function of alphaepithelial sodium channel(α-ENaC) as well as phosphorylation of cofilin,a family of actin-binding proteins which disassembles actin filaments,in mouse cortical collecting duct(mpkCCDc14) cells.The wild-type(WT)cofilin and its constitutively phosphorylated form(S3 D),but not its constitutively non-phosphorylable form(S3 A),contributed to the elevated expression on α-ENaC.Overexpression of 14-3-3ε,β,or y increased the expression of α-ENaC and cofilin phosphorylation,which was blunted by knockdown of 14-3-3ε,β,or y.Moreover,it was found that insulin increased the interaction between cofilin and 14-3-3 isoforms,which indicated relevance of 14-3-3 isoforms with cofilin.Furthermore,LIMK1/SSH1 pathway was involved in regulation of cofilin and α-ENaC expression by insulin.The results from this work indicate that cofilin participates in the regulation of α-ENaC by interaction with 14-3-3 isoforms.展开更多
Objective To investigate the correlations betweenthe expression of the slingshot-1(SSH1) and the generalclinicopathological factors in gastrointestinal stromaltumor (GIST) patients. Methods Immunohistochemicalstaining...Objective To investigate the correlations betweenthe expression of the slingshot-1(SSH1) and the generalclinicopathological factors in gastrointestinal stromaltumor (GIST) patients. Methods Immunohistochemicalstaining was used to detect the expression of SSH1 proteinin 96 gastrointestinal stromal tumors,and the correlationsbetween expression level of SSH1 and generalclinicopathological factors were analyzed. Results Totally,there were 96 cases of GIST patients (49 males and47 males),with a mean age of (55. 80 ± 12. 33) years.The tumors were located in different organs: 62 cases(64. 6%) in the stomach,29 cases (30. 2%) in the intestine,and 5 cases (5. 2%) in other organs,with amean tumor size of (5. 11 ± 3. 73) cm. The low riskgroup contained 57 cases ( 59. 4%), 18 cases(18. 8%) in middle risk group and 21 cases (21. 9%)in high risk group. The pathological examination showedthat the positive expression rate of CD117,CD34,DOG-1, Ki-67, SMA, S-100 and Desmin was 85. 4%,84. 4%,86. 5%,90. 6%,10. 4%,3. 1% and 2. 1%,respectively. In 96 cases of GIST,37 cases (38. 5%)had high expression of SSH1,and SSH1 expression levelwas correlated with the tumor invasion depth,risk classification,tumor mitotic figure count,CD34 ( + ) (P <0. 05).展开更多
基金supported by the National Natural Science Foundation of China to XL(Grant No.81870467 and No.81670619)。
文摘Renal epithelial sodium channel(ENaC) plays a crucial role in maintaining homeostasis and sodium absorption.While insulin participates in controlling sodium transport across the renal epithelium,the underlying molecular mechanism remain unclear.In this study,we found that insulin increased the expression and function of alphaepithelial sodium channel(α-ENaC) as well as phosphorylation of cofilin,a family of actin-binding proteins which disassembles actin filaments,in mouse cortical collecting duct(mpkCCDc14) cells.The wild-type(WT)cofilin and its constitutively phosphorylated form(S3 D),but not its constitutively non-phosphorylable form(S3 A),contributed to the elevated expression on α-ENaC.Overexpression of 14-3-3ε,β,or y increased the expression of α-ENaC and cofilin phosphorylation,which was blunted by knockdown of 14-3-3ε,β,or y.Moreover,it was found that insulin increased the interaction between cofilin and 14-3-3 isoforms,which indicated relevance of 14-3-3 isoforms with cofilin.Furthermore,LIMK1/SSH1 pathway was involved in regulation of cofilin and α-ENaC expression by insulin.The results from this work indicate that cofilin participates in the regulation of α-ENaC by interaction with 14-3-3 isoforms.
文摘Objective To investigate the correlations betweenthe expression of the slingshot-1(SSH1) and the generalclinicopathological factors in gastrointestinal stromaltumor (GIST) patients. Methods Immunohistochemicalstaining was used to detect the expression of SSH1 proteinin 96 gastrointestinal stromal tumors,and the correlationsbetween expression level of SSH1 and generalclinicopathological factors were analyzed. Results Totally,there were 96 cases of GIST patients (49 males and47 males),with a mean age of (55. 80 ± 12. 33) years.The tumors were located in different organs: 62 cases(64. 6%) in the stomach,29 cases (30. 2%) in the intestine,and 5 cases (5. 2%) in other organs,with amean tumor size of (5. 11 ± 3. 73) cm. The low riskgroup contained 57 cases ( 59. 4%), 18 cases(18. 8%) in middle risk group and 21 cases (21. 9%)in high risk group. The pathological examination showedthat the positive expression rate of CD117,CD34,DOG-1, Ki-67, SMA, S-100 and Desmin was 85. 4%,84. 4%,86. 5%,90. 6%,10. 4%,3. 1% and 2. 1%,respectively. In 96 cases of GIST,37 cases (38. 5%)had high expression of SSH1,and SSH1 expression levelwas correlated with the tumor invasion depth,risk classification,tumor mitotic figure count,CD34 ( + ) (P <0. 05).
