目的:从SRA信号通路研究黄芩苷对乙酰低密度脂蛋白(Ac-LDL)+脂多糖(LPS)诱导的与动脉粥样硬化(AS)相关的巨噬细胞凋亡的影响。方法:收集鼠腹腔巨噬细胞进行体外培养,使用100μg/m L Ac-LDL及1μg/m L LPS诱导巨噬细胞凋亡,以120、240、...目的:从SRA信号通路研究黄芩苷对乙酰低密度脂蛋白(Ac-LDL)+脂多糖(LPS)诱导的与动脉粥样硬化(AS)相关的巨噬细胞凋亡的影响。方法:收集鼠腹腔巨噬细胞进行体外培养,使用100μg/m L Ac-LDL及1μg/m L LPS诱导巨噬细胞凋亡,以120、240、480μg/m L黄芩苷作为低、中、高3种剂量进行干预,Annexin VFITC和PI双染后用流式细胞仪检测细胞凋亡,Western Blot技术检测SRA蛋白表达量。结果:与正常组比较,对照组与模型组的细胞凋亡率差异有统计学意义(P<0.01);低剂量观察组与模型组细胞凋亡率差异无统计学意义;中、高剂量观察组与模型组相比细胞凋亡率差异有统计学意义(P<0.01)。LPS对照组较Ac-LDL对照组的SRA含量显著增高(P<0.05),与黄芩苷低、中、高剂量组相比SRA表达量无统计学差异。结论:SRA信号通路参与了LPS和Ac-LDL双重因素诱导的巨噬细胞凋亡,并且SRA通路蛋白的激活主要与LPS干预相关。展开更多
The high grade non-oriented electrical steel sheets containing 3.0%Si were manufacturing processed using different cutting techniques, then they were stress relief annealed（SRA）, the profiles and textures of the cut...The high grade non-oriented electrical steel sheets containing 3.0%Si were manufacturing processed using different cutting techniques, then they were stress relief annealed（SRA）, the profiles and textures of the cutting edges were compared before and after annealing, and the magnetic properties of these specimens were tested and compared. The experimental results show that the iron loss of the specimen by water jet cutting is the lowest, but the magnetic induction under the low magnetic field is the highest, the iron loss of the specimen by laser cutting is the highest, but the magnetic induction under the low magnetic field is the lowest. It is necessary to adopt suitable production conditions and minimize the deterioration involved, and the magnetic property can be recovered by SRA effectively.展开更多
文摘目的:从SRA信号通路研究黄芩苷对乙酰低密度脂蛋白(Ac-LDL)+脂多糖(LPS)诱导的与动脉粥样硬化(AS)相关的巨噬细胞凋亡的影响。方法:收集鼠腹腔巨噬细胞进行体外培养,使用100μg/m L Ac-LDL及1μg/m L LPS诱导巨噬细胞凋亡,以120、240、480μg/m L黄芩苷作为低、中、高3种剂量进行干预,Annexin VFITC和PI双染后用流式细胞仪检测细胞凋亡,Western Blot技术检测SRA蛋白表达量。结果:与正常组比较,对照组与模型组的细胞凋亡率差异有统计学意义(P<0.01);低剂量观察组与模型组细胞凋亡率差异无统计学意义;中、高剂量观察组与模型组相比细胞凋亡率差异有统计学意义(P<0.01)。LPS对照组较Ac-LDL对照组的SRA含量显著增高(P<0.05),与黄芩苷低、中、高剂量组相比SRA表达量无统计学差异。结论:SRA信号通路参与了LPS和Ac-LDL双重因素诱导的巨噬细胞凋亡,并且SRA通路蛋白的激活主要与LPS干预相关。
基金Funded by the High Technology Research and Development Program of China(2011AA11A238)
文摘The high grade non-oriented electrical steel sheets containing 3.0%Si were manufacturing processed using different cutting techniques, then they were stress relief annealed（SRA）, the profiles and textures of the cutting edges were compared before and after annealing, and the magnetic properties of these specimens were tested and compared. The experimental results show that the iron loss of the specimen by water jet cutting is the lowest, but the magnetic induction under the low magnetic field is the highest, the iron loss of the specimen by laser cutting is the highest, but the magnetic induction under the low magnetic field is the lowest. It is necessary to adopt suitable production conditions and minimize the deterioration involved, and the magnetic property can be recovered by SRA effectively.
