To study biological activities of Duck Interferon Alpha （DuIFN-α） and prepare antivirus medicine, the eukaryotic expression vector of mature polypeptide of Duck Interferon Alpha （mDuIFN-α） gene was constructed a...To study biological activities of Duck Interferon Alpha （DuIFN-α） and prepare antivirus medicine, the eukaryotic expression vector of mature polypeptide of Duck Interferon Alpha （mDuIFN-α） gene was constructed and expressed in insect cell. By means of PCR technique, the mDuIFN-α gene was cloned from pMD-18-duIFN-α recombinant_ The gene was then inserted to pGEM-T vector and identified by restriction endonuclease analysis and sequencing The mDuIFN-α gene was ligated with the eukaryotic expression vector pMelBacA. then transfected into Sf9 cell line. Recombinant polypeptide was effectively expressed in insect cell and its molecular weight was 34 ku.展开更多
文摘To study biological activities of Duck Interferon Alpha （DuIFN-α） and prepare antivirus medicine, the eukaryotic expression vector of mature polypeptide of Duck Interferon Alpha （mDuIFN-α） gene was constructed and expressed in insect cell. By means of PCR technique, the mDuIFN-α gene was cloned from pMD-18-duIFN-α recombinant_ The gene was then inserted to pGEM-T vector and identified by restriction endonuclease analysis and sequencing The mDuIFN-α gene was ligated with the eukaryotic expression vector pMelBacA. then transfected into Sf9 cell line. Recombinant polypeptide was effectively expressed in insect cell and its molecular weight was 34 ku.
