核酸适配体是经过指数富集配体系统进化技术(systematic evolution of ligands by exponential enrichment,SELEX)筛选得到的能与金属离子、小分子、生物大分子,甚至整个细胞高专一性和亲和性结合的ssDNA或RNA分子。核酸适配体不仅具备...核酸适配体是经过指数富集配体系统进化技术(systematic evolution of ligands by exponential enrichment,SELEX)筛选得到的能与金属离子、小分子、生物大分子,甚至整个细胞高专一性和亲和性结合的ssDNA或RNA分子。核酸适配体不仅具备抗体分子的识别特性,凡是涉及抗体的诊断领域,几乎都可以用核酸适配体来代替,而且还具备自己独特的优异性能,所以对核酸适配体的研究引起了许多科学家的关注。本文介绍了核酸适配体及其相关筛选技术(SELEX),综述了核酸适配体近年来在治疗新药、药物运输、癌细胞检测、生物成像、生物标志物的发现、毒品检测、抗病毒等生物医学领域中的应用,最后对核酸适配体在生物医学上的应用前景进行了展望。展开更多
指数富集配体系统进化技术(Systematic evolution of ligand by exponential enrichment,SELEX)是近20年来出现的一种新型体外筛选技术。该技术将文库技术与筛选技术相结合,从核酸文库中筛选出的一段核酸分子:配基-适配子(aptamer);适...指数富集配体系统进化技术(Systematic evolution of ligand by exponential enrichment,SELEX)是近20年来出现的一种新型体外筛选技术。该技术将文库技术与筛选技术相结合,从核酸文库中筛选出的一段核酸分子:配基-适配子(aptamer);适配子与抗体类似,可以与相应靶物质特异性结合。SELEX技术筛选适配子的技术有快速的进步,而适配子也被应用到医学生物学等各个方面。在HIV等病毒学研究与治疗方面,SELEX的应用也有了可观的进展,多种病毒的相关适配子被筛选出来,为病毒相关疾病的防治提供了新的方向。本文主要就近三年来的SELEX技术发展状况及在应用上的发展进行简要综述。展开更多
Skeletal muscle disorders have posed great threats to health.Selective delivery of drugs and oligonucleotides to skeletal muscle is challenging.Aptamers can improve targeting efficacy.In this study,for the first time,...Skeletal muscle disorders have posed great threats to health.Selective delivery of drugs and oligonucleotides to skeletal muscle is challenging.Aptamers can improve targeting efficacy.In this study,for the first time,the human skeletal muscle-specific ssDNA aptamers(HSM01,etc.)were selected and identified with Systematic Evolution of Ligands by Exponential Enrichment(SELEX).The HSM01 ssDNA aptamer preferentially interacted with human skeletal muscle cells in vitro.The in vivo study using tree shrews showed that the HSM01 ssDNA aptamer specifically targeted human skeletal muscle cells.Furthermore,the ability of HSM01 ssDNA aptamer to target skeletal muscle cells was not affected by the formation of a disulfide bond with nanoliposomes in vitro or in vivo,suggesting a potential new approach for targeted drug delivery to skeletal muscles via liposomes.Therefore,this newly identified ssDNA aptamer and nanoliposome modification could be used for the treatment of human skeletal muscle diseases.展开更多
文摘核酸适配体是经过指数富集配体系统进化技术(systematic evolution of ligands by exponential enrichment,SELEX)筛选得到的能与金属离子、小分子、生物大分子,甚至整个细胞高专一性和亲和性结合的ssDNA或RNA分子。核酸适配体不仅具备抗体分子的识别特性,凡是涉及抗体的诊断领域,几乎都可以用核酸适配体来代替,而且还具备自己独特的优异性能,所以对核酸适配体的研究引起了许多科学家的关注。本文介绍了核酸适配体及其相关筛选技术(SELEX),综述了核酸适配体近年来在治疗新药、药物运输、癌细胞检测、生物成像、生物标志物的发现、毒品检测、抗病毒等生物医学领域中的应用,最后对核酸适配体在生物医学上的应用前景进行了展望。
文摘指数富集配体系统进化技术(Systematic evolution of ligand by exponential enrichment,SELEX)是近20年来出现的一种新型体外筛选技术。该技术将文库技术与筛选技术相结合,从核酸文库中筛选出的一段核酸分子:配基-适配子(aptamer);适配子与抗体类似,可以与相应靶物质特异性结合。SELEX技术筛选适配子的技术有快速的进步,而适配子也被应用到医学生物学等各个方面。在HIV等病毒学研究与治疗方面,SELEX的应用也有了可观的进展,多种病毒的相关适配子被筛选出来,为病毒相关疾病的防治提供了新的方向。本文主要就近三年来的SELEX技术发展状况及在应用上的发展进行简要综述。
基金supported by the National Key Research and Development Plan(2018YFC2001500)National Natural Science Foundation of China(81972254,82172098).
文摘Skeletal muscle disorders have posed great threats to health.Selective delivery of drugs and oligonucleotides to skeletal muscle is challenging.Aptamers can improve targeting efficacy.In this study,for the first time,the human skeletal muscle-specific ssDNA aptamers(HSM01,etc.)were selected and identified with Systematic Evolution of Ligands by Exponential Enrichment(SELEX).The HSM01 ssDNA aptamer preferentially interacted with human skeletal muscle cells in vitro.The in vivo study using tree shrews showed that the HSM01 ssDNA aptamer specifically targeted human skeletal muscle cells.Furthermore,the ability of HSM01 ssDNA aptamer to target skeletal muscle cells was not affected by the formation of a disulfide bond with nanoliposomes in vitro or in vivo,suggesting a potential new approach for targeted drug delivery to skeletal muscles via liposomes.Therefore,this newly identified ssDNA aptamer and nanoliposome modification could be used for the treatment of human skeletal muscle diseases.
