为研究疏水蛋白融合表达对脂肪酶的催化性质影响,分别构建分泌表达解脂耶氏酵母脂肪酶Lip2,带His标签Lip2及Lip2与疏水蛋白SC3融合蛋白的重组质粒PKL、PKHL和PKHLS并分别转化到毕赤酵母GS115菌株中,通过筛选,获得表达活性较高的工程菌株...为研究疏水蛋白融合表达对脂肪酶的催化性质影响,分别构建分泌表达解脂耶氏酵母脂肪酶Lip2,带His标签Lip2及Lip2与疏水蛋白SC3融合蛋白的重组质粒PKL、PKHL和PKHLS并分别转化到毕赤酵母GS115菌株中,通过筛选,获得表达活性较高的工程菌株(KL、KHL、KHLS)。对3株重组菌株的培养与重组蛋白催化性质研究表明蛋白相对分子质量为36 k Da、36 k Da、46 k Da,发酵液对底物对硝基苯酚辛酸酯(p-NPC)水解酶活分别达到5.35、4.69、2.4 U/m L。最适温度均是45℃,最适p H均是7.5,另外KHLS菌株的酶活力受表面活性剂的影响比KL和KHL小。展开更多
The photoluminescence (PL) property effect of Sc3+ on the Er3+/Y3+/Yb3+ doped Al2O3 powders prepared by sol-gel method has been investigated. The X-ray diffraction (XRD) and transmission electron microscopy (...The photoluminescence (PL) property effect of Sc3+ on the Er3+/Y3+/Yb3+ doped Al2O3 powders prepared by sol-gel method has been investigated. The X-ray diffraction (XRD) and transmission electron microscopy (TEM) implied that the distribution of dopants (Er3+ , Y3+ , Yb3+ , Sc3+) was improved effectively with the rise of Sc3+ concentration. The Fourier transform infrared spectra (FTIR) results demonstrated that the ligand around the quenching center -OH and the population of -OH were altered by introducing different amounts of Sc3+ . The PL intensity centered at 1530 nm was increasingly improved with the rise of Sc3+ concentration, as well as the corresponding full widths at the half maximum (FWHM) and lifetime. The optimized PL intensity was 4.7 times higher than that non-Sc3+ doped sample for the Al2O3 powders codoped with 10mol% Sc3+ . This material can be promising candidates for optical fiber amplifier.展开更多
文摘为研究疏水蛋白融合表达对脂肪酶的催化性质影响,分别构建分泌表达解脂耶氏酵母脂肪酶Lip2,带His标签Lip2及Lip2与疏水蛋白SC3融合蛋白的重组质粒PKL、PKHL和PKHLS并分别转化到毕赤酵母GS115菌株中,通过筛选,获得表达活性较高的工程菌株(KL、KHL、KHLS)。对3株重组菌株的培养与重组蛋白催化性质研究表明蛋白相对分子质量为36 k Da、36 k Da、46 k Da,发酵液对底物对硝基苯酚辛酸酯(p-NPC)水解酶活分别达到5.35、4.69、2.4 U/m L。最适温度均是45℃,最适p H均是7.5,另外KHLS菌株的酶活力受表面活性剂的影响比KL和KHL小。
基金supported by the Natural Science Foundation of Fujian Province (No. 2008J0146)
文摘The photoluminescence (PL) property effect of Sc3+ on the Er3+/Y3+/Yb3+ doped Al2O3 powders prepared by sol-gel method has been investigated. The X-ray diffraction (XRD) and transmission electron microscopy (TEM) implied that the distribution of dopants (Er3+ , Y3+ , Yb3+ , Sc3+) was improved effectively with the rise of Sc3+ concentration. The Fourier transform infrared spectra (FTIR) results demonstrated that the ligand around the quenching center -OH and the population of -OH were altered by introducing different amounts of Sc3+ . The PL intensity centered at 1530 nm was increasingly improved with the rise of Sc3+ concentration, as well as the corresponding full widths at the half maximum (FWHM) and lifetime. The optimized PL intensity was 4.7 times higher than that non-Sc3+ doped sample for the Al2O3 powders codoped with 10mol% Sc3+ . This material can be promising candidates for optical fiber amplifier.