摘要
目的 :探讨重组人IFN -γ(rhIFN -γ)在体外对SW1116抗原SC3A表达的影响。方法 :将rhIFN -γ与SW1116在37℃共育24、48和72h后 ,分别用免疫细胞化学染色法和流式细胞仪测定其SC3A抗原 (SC3AAg)的表达。结果:SW1116与20u/mlrhIFN -γ共育24、48和72h ,其SC3AAg 阳性细胞分别为95.5 %、93.2 %和95.2 %,PBS组分别为11.3 %、12.2 %和10.0 % ,AGZY -83 -a细胞系均未见SC3AAg 阳性细胞 ,阳性对照组的C1184细胞系SC3AAg阳性细胞80.5 %。结论 :2u/mlrhIFN -γ能使SW1116细胞的SC3AAg表达明显上调 ,其中20u/ml、24h是表达峰值的最佳浓度和时间。
Objective:To observe regulation of the tumor associated antigen SC3A(TAASC3A)on cell line SW1116,human colon carcinoma,using rhIFN-γin vitro.Method:The cell line SW1116 were induced with rhIFN-γ.The percentage of positive cells with antigen SC3A(SC3AAg)expressing was determined by immunohistochemistry staining and Flow cytometry(FCM)respectively.Results:The positive cells of SC3AAgexpressing on/in SW1116 induced by 20u/ml rhIFN-γwere 95.5%,93.2%and 95.2%respectively at 24,48 and 72 h.SC3AAg positive cells of SW1116 without rhIFN-γwere 11.3%,12.2%and 10.0%respectively.No SC3AAgexpressing on AGZY83a was observed.Conclusion:2u/ml of rhIFNγcan regulate up SC3AAg on SW1116 obviously.It was showed that 20 u/ml of rhIFN-γfor 24h was optimum concentration and time.
出处
《现代康复》
CSCD
2000年第6期862-863,共2页
Modern Rehabilitation
基金
辽宁省自然科学基金资助项目!(962251)
