The effect of culture conditions on the production of bioactive secondary metabolites by the endophytic fungus Arthrinium State of Apiospora Montagnei Sacc. was investigated. Culture broths were partitioned with ethyl...The effect of culture conditions on the production of bioactive secondary metabolites by the endophytic fungus Arthrinium State of Apiospora Montagnei Sacc. was investigated. Culture broths were partitioned with ethyl acetate and the resulting extracts were evaluated for antibacterial, antifungal, cytotoxic, and antiparasitic activities. The highest levels of activities were arisen from cultures cultivated at 30。C in modified Czapek medium. The best antimicrobial activity against Escherichia coli and Pseudomonas aeruginosa (MIC 90 μg/ml), and against Aspergillus fumigatus (MIC 130 μg/ml) were found in extracts from cultures grown in medium containing 3.1% (w/v) sucrose and 0.1% (w/v) sodium nitrate at pH 4.0 after 9 days of incubation. Cultures grown in medium without modification also showed antiparasitic and antitumoral activities after 9 days at pH 4.0, antifungal activity against Candida albicans at pH 4.0 - 7.0 (MIC 140 μg/ml), but against A. fumigatus just after 27 days of incubation. A specific bioactive compound may have its production improved if the culture conditions parameters that affect or influence the production were known, which in turn makes the purification process easier.展开更多
[ Objective] The aim was to identify the pathogen of Gastrodia elata white silk disease and explore prevention and treatment of the disease.[ Method] Infected G. elata, collected from low-mountain regions in Yichang c...[ Objective] The aim was to identify the pathogen of Gastrodia elata white silk disease and explore prevention and treatment of the disease.[ Method] Infected G. elata, collected from low-mountain regions in Yichang city of Hubei Province, were isolated and purified. Then, Koch＇s Postulate was adopted to inoculate the pathogen. Finally, the identification was carried out according to its biological characteristics. [ Result] The pathogen of white silk disease belongs to weak hyperparasite. Because of its poor direct invasion ability, the pathogen usually invades into host through wound infection. According to its hyphae morphology and pathogenicity, the pathogen of G. elata White Silk Disease has been identified as Sc/erotium ro/fsii Sacc., which belongs to deuteromycotina, hyphomycetes, agonomycetales, fungi of sclerotium genus. [ Conclusion ] The study will provide references for the control of G. elata White Silk Disease caused by S. rolfsii.展开更多
文摘The effect of culture conditions on the production of bioactive secondary metabolites by the endophytic fungus Arthrinium State of Apiospora Montagnei Sacc. was investigated. Culture broths were partitioned with ethyl acetate and the resulting extracts were evaluated for antibacterial, antifungal, cytotoxic, and antiparasitic activities. The highest levels of activities were arisen from cultures cultivated at 30。C in modified Czapek medium. The best antimicrobial activity against Escherichia coli and Pseudomonas aeruginosa (MIC 90 μg/ml), and against Aspergillus fumigatus (MIC 130 μg/ml) were found in extracts from cultures grown in medium containing 3.1% (w/v) sucrose and 0.1% (w/v) sodium nitrate at pH 4.0 after 9 days of incubation. Cultures grown in medium without modification also showed antiparasitic and antitumoral activities after 9 days at pH 4.0, antifungal activity against Candida albicans at pH 4.0 - 7.0 (MIC 140 μg/ml), but against A. fumigatus just after 27 days of incubation. A specific bioactive compound may have its production improved if the culture conditions parameters that affect or influence the production were known, which in turn makes the purification process easier.
基金Supported by Ministry of Science,"Agriculture Science and Technolo-gy Achievements Capital Projects"(02EFN216700804)Yichang Key Research Funded Projects(A03209-4)~~
文摘[ Objective] The aim was to identify the pathogen of Gastrodia elata white silk disease and explore prevention and treatment of the disease.[ Method] Infected G. elata, collected from low-mountain regions in Yichang city of Hubei Province, were isolated and purified. Then, Koch＇s Postulate was adopted to inoculate the pathogen. Finally, the identification was carried out according to its biological characteristics. [ Result] The pathogen of white silk disease belongs to weak hyperparasite. Because of its poor direct invasion ability, the pathogen usually invades into host through wound infection. According to its hyphae morphology and pathogenicity, the pathogen of G. elata White Silk Disease has been identified as Sc/erotium ro/fsii Sacc., which belongs to deuteromycotina, hyphomycetes, agonomycetales, fungi of sclerotium genus. [ Conclusion ] The study will provide references for the control of G. elata White Silk Disease caused by S. rolfsii.
