为建立针对韭菜迟眼蕈蚊Bradysia odoriphaga Yang et Zhang幼虫(韭蛆)的可靠生测方法,采用胃毒触杀联合毒力法、人工饲料混药法和浸虫法分别测定毒死蜱、噻虫胺对不同龄期韭蛆的毒力,以明确龄期、饲养温度、食料及测定方法对药剂敏感...为建立针对韭菜迟眼蕈蚊Bradysia odoriphaga Yang et Zhang幼虫(韭蛆)的可靠生测方法,采用胃毒触杀联合毒力法、人工饲料混药法和浸虫法分别测定毒死蜱、噻虫胺对不同龄期韭蛆的毒力,以明确龄期、饲养温度、食料及测定方法对药剂敏感性的影响。试验结果表明,上述三种方法测得2龄幼虫对毒死蜱的敏感性分别为4龄幼虫的11.9倍、7.4倍、3.3倍;毒死蜱对取食韭菜、人工饲料、大葱韭蛆的LC50分别为圆葱的1.84倍、1.47倍、2.31倍;在15-30℃范围内,随韭蛆饲养温度升高,毒死蜱对4龄幼虫的LC50值增大;三种方法测得毒死蜱对4龄幼虫的LC50值分别为67.199 mg/L、71.123 mg/L、88.120 mg/L,噻虫胺对4龄幼虫的LC50值依次为3.584 mg/L、2.255 mg/L、4.329mg/L,胃毒触杀联合毒力法和人工饲料混药法的毒力曲线拟合度高于浸虫法。说明龄期、饲养温度、食料、测定方法均影响韭蛆对杀虫剂的敏感性。展开更多
Dried fruit beetles can be cultured easily using an artificial diet,therefore it is notnecessary to provide fruit.The ingredient are:Torula yeast,54g:Dried carrot,162g;Nipagin,2.4g;HCL Conc.7ml;Rain water,800ml.About6...Dried fruit beetles can be cultured easily using an artificial diet,therefore it is notnecessary to provide fruit.The ingredient are:Torula yeast,54g:Dried carrot,162g;Nipagin,2.4g;HCL Conc.7ml;Rain water,800ml.About60g of diet in pressed against one side of ahalf litre yogurt and sprinkled with a teaspoonful of active yeast.Two teaspoons full of diet ontop of the gauze lid and sprinkled with active yeast granules for the adults to eat and also to layeggs into.Up to2000 larvae should be produced from400 parents each week.About200 eggscan be collected,when making observations.The culture is rejuvenated each year with beetlescollected from the wild,kept isolated to avoid contamination of the culture room with mites.展开更多
文摘为建立针对韭菜迟眼蕈蚊Bradysia odoriphaga Yang et Zhang幼虫(韭蛆)的可靠生测方法,采用胃毒触杀联合毒力法、人工饲料混药法和浸虫法分别测定毒死蜱、噻虫胺对不同龄期韭蛆的毒力,以明确龄期、饲养温度、食料及测定方法对药剂敏感性的影响。试验结果表明,上述三种方法测得2龄幼虫对毒死蜱的敏感性分别为4龄幼虫的11.9倍、7.4倍、3.3倍;毒死蜱对取食韭菜、人工饲料、大葱韭蛆的LC50分别为圆葱的1.84倍、1.47倍、2.31倍;在15-30℃范围内,随韭蛆饲养温度升高,毒死蜱对4龄幼虫的LC50值增大;三种方法测得毒死蜱对4龄幼虫的LC50值分别为67.199 mg/L、71.123 mg/L、88.120 mg/L,噻虫胺对4龄幼虫的LC50值依次为3.584 mg/L、2.255 mg/L、4.329mg/L,胃毒触杀联合毒力法和人工饲料混药法的毒力曲线拟合度高于浸虫法。说明龄期、饲养温度、食料、测定方法均影响韭蛆对杀虫剂的敏感性。
文摘Dried fruit beetles can be cultured easily using an artificial diet,therefore it is notnecessary to provide fruit.The ingredient are:Torula yeast,54g:Dried carrot,162g;Nipagin,2.4g;HCL Conc.7ml;Rain water,800ml.About60g of diet in pressed against one side of ahalf litre yogurt and sprinkled with a teaspoonful of active yeast.Two teaspoons full of diet ontop of the gauze lid and sprinkled with active yeast granules for the adults to eat and also to layeggs into.Up to2000 larvae should be produced from400 parents each week.About200 eggscan be collected,when making observations.The culture is rejuvenated each year with beetlescollected from the wild,kept isolated to avoid contamination of the culture room with mites.
