摘要
目的通过观察普通饲养组、高脂饲养组和限制饲养组的吸烟和非吸烟大鼠肺气肿、骨质疏松程度及血清肿瘤坏死因子a（TNF-a）、IL-6和脂联素（APN）水平，探讨不同饲养方式和吸烟所致大鼠肺气肿合并骨质疏松的程度及APN在其过程中的作用。方法48只雄性SD大鼠，按吸烟与否及饲养方式随机分为普通饲养组、高脂饲养组、限制饲养组、普通饲养吸烟组、高脂饲养吸烟组和限制饲养吸烟组，分别给予空气、香烟烟雾暴露及相应饲养方式。6个月后测量大鼠Lee’S指数，HE染色测量肺平均内衬间隔（Mu）、单位面积平均肺泡数（MAN）、骨小梁面积比（Tb．Ar）、骨小梁数目（Tb．N）和骨小梁间隔（Tb．Sp），ELISA法测定血清TNF-a、IL-6、APN和骨钙素（BGP）水平。结果①与非吸烟大鼠比较，吸烟大鼠Lee’s指数降低。吸烟及非吸烟大鼠均表现为高脂饲养组Lee’S指数最高，限制饲养组Lee’S指数最低，差异均有统计学意义（P〈0．05）。②与非吸烟大鼠比较，吸烟大鼠MLI增高，MAN降低。吸烟大鼠组内比较：限制饲养组MLI[（102．71±11．29）gm]最高、MAN[（130．85±19．8）个／mm2]最低，差异均有统计学意义（P〈0．05）。③与非吸烟大鼠比较，吸烟大鼠Tb．Ar、Tb．N、BGP降低，Tb．Sp增高，吸烟大鼠组内比较：高脂饲养组Tb．Ar[（14．23±1．08）％]、Tb．N[（3．04±0．30）N／mm2]、BGP[（296．15±2．81）μg／L]水平最高，Tb．Sp[（270．66±30．33）m1]最低，限制饲养组Tb．Ar[（7．96±1．41）％]、Tb．N[（2．n±0．25）N／mm2]、BGP[（275．72±3．44）μg／L]水平最低，Tb．sp[（445．05±28．17）μ]最高，差异均有统计学意义（P〈O．05）。④与非吸烟大鼠比较，吸烟大鼠血清TNF-a和IL-6水平增高。吸烟大鼠组内比较，高脂饲养组和限制饲养组血清TNF—a和IL-6水平均高于普通饲养组，差异均有�
Objective By observing the degree of emphysema and osteoporosis, and the serum tumor necrosis factor （TNF）-a, interieukin （IL）-6 and adiponectin（APN） levels of rats, which were fed with normal diet, fat-rich diet or limited intake diet and exposed to tobacco smoke or room air, we aim to explore the role of APN in the process of emphysema rats with osteoporosis induced by different ways of rearing and tobacco smoke. Methods Forty-eight 6-week clean male SD rats were randomly divded into normal diet＋ smoking group, fat-rich diet ＋ smoking group, limited intake ＋ smoking group, normal diet group, fat-rich diet group, and limited intake group, which were exposed to tobacco smoke or room air and fed with corresponding diet. The relevant indices were measured in each groups after 6 months. Lee＇ s index was calculated. Mean linear intercept （MLI）, mean alveoli number （MAN）, Trabeeular Bone area ratio （Tb. At）, the number of bone trabeculae （Tb. N） and the bone trabecula space （Tb. Sp） were calculated by the microscope. The serum TNF-a, IL-6, APN and osteoealcin （BGP） levels were detected by ELISA kits. Results ①Smoking made rats＇ Lee＇s index decreasing. Both in non-smoking group and smoking rats, Lee＇s index in fat-rich diet group was the highest, and limited intake group＇ Lee＇s index was the least. The differences were all significant （all P 〈 0.05）. Q Smoking made rats＇ MLI increasing and MAN decreasing. In smoking rats, the MLI （102.71 ±11.29） μm in limited intake group was the highest and MAN （130.85±19.8）/mm2 was the lowest,and fat-rich diet group＇ MLI was the lowest and MAN was the highest, the differences were all significant （all P 〈0.05）. @）Smoking made rats＇ Tb. Ar, Tb. N, BGP decreasing and Tb. Sp increasing. In smoking rats, Tb. Ar （14.23 ± 1.08）%, Tb. N （3.04 ± 0.30）/mm2 and BGP （296.15 ± 2.81） g/L in fat-rich diet group were the highest, Tb. Sp （270.66 ± 30.33） g/L was the lowest. Tb. Ar （7.96± 1.4
出处
《国际呼吸杂志》
2016年第5期376-381,共6页
International Journal of Respiration
关键词
饲养方式
吸烟
肺气肿
骨质疏松
脂联素
Rearing methods
Tobacco smoke
Emphysema
Osteoporosis
Adiponeetin
