Recent studies have uncovered two signaling pathways that activate the host innate immunity against viral infection. One of the pathways utilizes members of the Toll-like receptor （TLR） family to detect viruses that...Recent studies have uncovered two signaling pathways that activate the host innate immunity against viral infection. One of the pathways utilizes members of the Toll-like receptor （TLR） family to detect viruses that enter the endosome through endocytosis. The TLR pathway induces interferon production through several signaling proteins that ultimately lead to the activation of the transcription factors NF-kB, IRF3 and IRFT. The other antiviral pathway uses the RNA helicase RIG-Ⅰ as the receptor for intracellular viral double-stranded RNA. RIG-Ⅰ activates NF-kB and IRFs through the recently identified adaptor protein MAVS, a CARD domain containing protein that resides in the mitochondrial membrane. MAVS is essential for antiviral innate immunity, but it also serves as a target of Hepatitis C virus （HCV）, which employs a viral protease to cleave MAVS off the mitochondria, thereby allowing HCV to escape the host immune system.展开更多
目的研究环指蛋白34(RING finger protein 34,RNF34)对天然免疫的调控。方法利用重组PCR方法构建pcDNA3-Flag-RNF34、pcDNA3-Flag-RNF34-ΔFYVE、pcDNA3-Flag-RNF34-ΔCID和pcDNA3-Flag-RNF34-ΔRING质粒,并在HEK293T细胞中瞬时表达;利...目的研究环指蛋白34(RING finger protein 34,RNF34)对天然免疫的调控。方法利用重组PCR方法构建pcDNA3-Flag-RNF34、pcDNA3-Flag-RNF34-ΔFYVE、pcDNA3-Flag-RNF34-ΔCID和pcDNA3-Flag-RNF34-ΔRING质粒,并在HEK293T细胞中瞬时表达;利用双荧光素酶报告基因技术检测RNF34及其3种突变体对仙台病毒(SeV)和N-RIG-Ⅰ激活NF-κB和IFN-β转录活性的影响。结果成功构建了Flag-RNF34及其3种结构域缺失突变体的真核表达质粒;发现在SeV刺激下,RNF34对NF-κB和IFN-β转录活性有明显抑制作用,RNF34-ΔFYVE、RNF34-ΔCID和RNF34-ΔRING与RNF34相比此种抑制作用减弱。同时发现对于N-RIG-Ⅰ激活的NF-κB和IFN-β活性,RNF34及其3种结构域缺失突变体也有相似的抑制作用。结论 RNF34通过负调控RIG-Ⅰ-MAVS信号通路调控天然免疫。展开更多
RIG-I样受体(RIG-I like receptors,RLRs)是天然免疫系统中一类重要模式识别受体,在细胞天然免疫应答中发挥重要的作用,LGP2是RLRs家族成员之一,对RLRs信号转导有正向和负向调控的双重作用。LGP2依据感染病毒种类的不同发挥着不同的调...RIG-I样受体(RIG-I like receptors,RLRs)是天然免疫系统中一类重要模式识别受体,在细胞天然免疫应答中发挥重要的作用,LGP2是RLRs家族成员之一,对RLRs信号转导有正向和负向调控的双重作用。LGP2依据感染病毒种类的不同发挥着不同的调控作用,但详细作用机制不明确。随着研究的深入,LGP2在天然免疫应答中的作用愈发重要。本文就近年来对LGP2参与RLRs介导的抗病毒天然免疫应答的调控及在不同病毒感染宿主中所起的作用作一综述,以期为LGP2深入研究提供参考。展开更多
文摘Recent studies have uncovered two signaling pathways that activate the host innate immunity against viral infection. One of the pathways utilizes members of the Toll-like receptor （TLR） family to detect viruses that enter the endosome through endocytosis. The TLR pathway induces interferon production through several signaling proteins that ultimately lead to the activation of the transcription factors NF-kB, IRF3 and IRFT. The other antiviral pathway uses the RNA helicase RIG-Ⅰ as the receptor for intracellular viral double-stranded RNA. RIG-Ⅰ activates NF-kB and IRFs through the recently identified adaptor protein MAVS, a CARD domain containing protein that resides in the mitochondrial membrane. MAVS is essential for antiviral innate immunity, but it also serves as a target of Hepatitis C virus （HCV）, which employs a viral protease to cleave MAVS off the mitochondria, thereby allowing HCV to escape the host immune system.
文摘目的研究环指蛋白34(RING finger protein 34,RNF34)对天然免疫的调控。方法利用重组PCR方法构建pcDNA3-Flag-RNF34、pcDNA3-Flag-RNF34-ΔFYVE、pcDNA3-Flag-RNF34-ΔCID和pcDNA3-Flag-RNF34-ΔRING质粒,并在HEK293T细胞中瞬时表达;利用双荧光素酶报告基因技术检测RNF34及其3种突变体对仙台病毒(SeV)和N-RIG-Ⅰ激活NF-κB和IFN-β转录活性的影响。结果成功构建了Flag-RNF34及其3种结构域缺失突变体的真核表达质粒;发现在SeV刺激下,RNF34对NF-κB和IFN-β转录活性有明显抑制作用,RNF34-ΔFYVE、RNF34-ΔCID和RNF34-ΔRING与RNF34相比此种抑制作用减弱。同时发现对于N-RIG-Ⅰ激活的NF-κB和IFN-β活性,RNF34及其3种结构域缺失突变体也有相似的抑制作用。结论 RNF34通过负调控RIG-Ⅰ-MAVS信号通路调控天然免疫。
文摘RIG-I样受体(RIG-I like receptors,RLRs)是天然免疫系统中一类重要模式识别受体,在细胞天然免疫应答中发挥重要的作用,LGP2是RLRs家族成员之一,对RLRs信号转导有正向和负向调控的双重作用。LGP2依据感染病毒种类的不同发挥着不同的调控作用,但详细作用机制不明确。随着研究的深入,LGP2在天然免疫应答中的作用愈发重要。本文就近年来对LGP2参与RLRs介导的抗病毒天然免疫应答的调控及在不同病毒感染宿主中所起的作用作一综述,以期为LGP2深入研究提供参考。
