摘要
为揭示野生绿头鸭视黄酸诱导基因Ⅰ(Retinoic acid-inducible geneⅠ,RIG-Ⅰ)的分子特征及其在体内的表达分布规律,对野生绿头鸭RIG-Ⅰ(MdRIG-Ⅰ)基因的CDS序列进行了克隆和序列分析,并应用荧光定量PCR方法对RIG-Ⅰ基因在绿头鸭不同组织中的表达水平进行了测定。结果显示:MdRIG-Ⅰ基因的CDS序列全长2 802 bp,编码933个氨基酸,与哺乳动物和鱼类的RIG-Ⅰ基因同源率较低,为43.7%~46.7%与52.2%~54.9%;与鸟类的RIG-Ⅰ基因同源率较高,为78.7%~99.8%;与番鸭和绍兴麻鸭的RIG-Ⅰ基因同源率为98.3%和99.8%,有较强的种间特异性;MdRIG-Ⅰ基因mRNA在体内不同器官的表达量均高于SPF鸭(绍兴麻鸭),且不同组织表达量各不相同:在肝脏中表达水平最高,其次是肠,在心和脾表达呈中等水平,在肺和肾表达呈低等水平。研究结果揭示了RIG-Ⅰ通路在鸭科动物抗病毒中的作用及其分子机制,为水禽先天性免疫研究奠定良好的基础。
In order to reveal the molecular characteristic and expression distribution discipline of Retinoic acid-inducible gene Ⅰ(RIG-Ⅰ) of wild mallards(Anas platyrhynchos), wild mallards RIG-Ⅰ(MdRIG-Ⅰ) gene CDS sequence was cloned and sequenced. Fluorescent quantitative PCR was used to detect the expression level of RIG-Ⅰ gene in different tissues of wild mallards. The results showed that the CDS sequence of MdRIG-Ⅰ gene with a total length of 2 802 bp,which encodes 933 amino acids, MdRIG-Ⅰ gene had low similarity to mammals and fish, from 43.7% to 46.7% and 52.2% to 54.3%, respectively. MdRIG-Ⅰ gene had high homologous with genes induced by birds, from 78.7% to 99.8%. The homology of RIG-Ⅰ gene between wild mallards and Muscovy duck and wild mallards and Shaoxing Ma Duck was 98.3% and 99.8%, respectively, which indicated that RIG-Ⅰ gene had a strong interspecies specificity. The mRNA expression level of RIG-Ⅰ gene in different tissues of wild mallards were higher than that in SPF duck and the mRNA expression of RIG-Ⅰ gene was different among different tissues, with the highest expression in liver,followed by the duodenum, with medium expression in the heart and spleen, with low expression in the lungs and kidneys. These findings revealled the role and molecular mechanism of RIG-Ⅰ pathway in antiviral infections of ducks, which would lay the foundation for the study on waterfowl innate immunity.
作者
朱晓艺
翟博宇
李靖
林圣泽
曾祥伟
ZHU Xiaoyi;ZHAI Boyu;LI Jing;LIN Shengze;ZENG Xiangwei(College of Wildlife and Protected Area,Northeast Forestry University,Harbin,Heilongjiang 150000)
出处
《中国家禽》
北大核心
2020年第11期33-37,共5页
China Poultry
基金
黑龙江省自然科学基金项目(LH2019C13)
国家林业和草原局野生动物疫病监测与预警系统维护项目(2019076018)。
关键词
野生绿头鸭
RIG-Ⅰ
分子特征
表达分布
wild mallards
RIG-Ⅰ
molecular characteristic
expression distribution
