Objective: To analyze the application value of combined detection of sperm quality, sex hormone and ovulation in the diagnosis of infertility. Methods: The study was conducted from June 2021 to June 2022. Sixty infert...Objective: To analyze the application value of combined detection of sperm quality, sex hormone and ovulation in the diagnosis of infertility. Methods: The study was conducted from June 2021 to June 2022. Sixty infertile couples who received IVF cycle treatment in our hospital during this period were selected as the observation group, and 60 couples with good sperm quality and follicle number ≥ 5 who conceived naturally after gynecological disease treatment were selected as the control group during the same period. The sperm quality, sex hormones and ovulation of the two groups were observed, and the change of positive rate was detected by the combined detection method. Results: Compared with the control group, the observation group had less semen (2.82 ± 0.12) ml, lower concentration (69.17 ± 1.28) × 106/ml, normal sperm morphology rate (2.92% ± 0.11%), lower survival rate (70.25% ± 1.16%), higher deformed sperm index (1.39 ± 0.11), and significant differences between groups (P < 0.05);The levels of FSH (7.15 ± 1.33) U/L, LH (5.13 ± 0.53) mU/ml, E2 (72.34 ± 5.11) ng/L, AMH (3.87 ± 0.67) ng/ml and AFC (7.15 ± 0.76) in the control group were significantly better than those in the observation group (P < 0.05). Compared with the single detection method of the three groups, the positive detection rate of the combined diagnosis method was higher, and the difference between the groups was significant (P < 0.05). Conclusion: For the diagnosis of infertility, the combined detection method of sperm quality, sex hormone and ovulation can effectively clarify the problems existing in the couple, significantly improve the detection rate of the cause of the patient, and is more conducive to guiding clinical symptomatic treatment, which is worthy of promotion and reference.展开更多
本研究探讨不同途径给羊接种布鲁氏菌S2菌苗后抗体消长规律,以及用4种方法检测菌苗抗体结果的符合程度。选取44只在相同饲养条件下的北京土种绵羊进行单次免疫,分为注射组20只,50×108CFU/只;灌喂组24只,100×108CFU/只。在免...本研究探讨不同途径给羊接种布鲁氏菌S2菌苗后抗体消长规律,以及用4种方法检测菌苗抗体结果的符合程度。选取44只在相同饲养条件下的北京土种绵羊进行单次免疫,分为注射组20只,50×108CFU/只;灌喂组24只,100×108CFU/只。在免疫后的第3、7、13、17、21、25周采血分离血清,用虎红平板凝集试验(RBPT),试管凝集试验(SAT),微量凝集试验(MAT)和酶联免疫吸附试验(ELISA)方法,对这44份血清抗体进行检测并分析。结果显示:注射组在第7周仍能保持一定的抗体水平,直至第25周仍能检测到抗体,而灌喂组在第13周阳性率已为0。3种检测方法与ELISA比较κ值分别为:κRBPT vs ELISA=0.805,κSAT vs ELISA=0.760,κMAT vs ELISA=0.899。肌肉注射比灌喂接种方式有更高的抗体阳性率,且该抗体能维持半年。微量凝集试验与酶联免疫吸附试验抗体检测结果的一致性最高。展开更多
文摘Objective: To analyze the application value of combined detection of sperm quality, sex hormone and ovulation in the diagnosis of infertility. Methods: The study was conducted from June 2021 to June 2022. Sixty infertile couples who received IVF cycle treatment in our hospital during this period were selected as the observation group, and 60 couples with good sperm quality and follicle number ≥ 5 who conceived naturally after gynecological disease treatment were selected as the control group during the same period. The sperm quality, sex hormones and ovulation of the two groups were observed, and the change of positive rate was detected by the combined detection method. Results: Compared with the control group, the observation group had less semen (2.82 ± 0.12) ml, lower concentration (69.17 ± 1.28) × 106/ml, normal sperm morphology rate (2.92% ± 0.11%), lower survival rate (70.25% ± 1.16%), higher deformed sperm index (1.39 ± 0.11), and significant differences between groups (P < 0.05);The levels of FSH (7.15 ± 1.33) U/L, LH (5.13 ± 0.53) mU/ml, E2 (72.34 ± 5.11) ng/L, AMH (3.87 ± 0.67) ng/ml and AFC (7.15 ± 0.76) in the control group were significantly better than those in the observation group (P < 0.05). Compared with the single detection method of the three groups, the positive detection rate of the combined diagnosis method was higher, and the difference between the groups was significant (P < 0.05). Conclusion: For the diagnosis of infertility, the combined detection method of sperm quality, sex hormone and ovulation can effectively clarify the problems existing in the couple, significantly improve the detection rate of the cause of the patient, and is more conducive to guiding clinical symptomatic treatment, which is worthy of promotion and reference.
文摘本研究探讨不同途径给羊接种布鲁氏菌S2菌苗后抗体消长规律,以及用4种方法检测菌苗抗体结果的符合程度。选取44只在相同饲养条件下的北京土种绵羊进行单次免疫,分为注射组20只,50×108CFU/只;灌喂组24只,100×108CFU/只。在免疫后的第3、7、13、17、21、25周采血分离血清,用虎红平板凝集试验(RBPT),试管凝集试验(SAT),微量凝集试验(MAT)和酶联免疫吸附试验(ELISA)方法,对这44份血清抗体进行检测并分析。结果显示:注射组在第7周仍能保持一定的抗体水平,直至第25周仍能检测到抗体,而灌喂组在第13周阳性率已为0。3种检测方法与ELISA比较κ值分别为:κRBPT vs ELISA=0.805,κSAT vs ELISA=0.760,κMAT vs ELISA=0.899。肌肉注射比灌喂接种方式有更高的抗体阳性率,且该抗体能维持半年。微量凝集试验与酶联免疫吸附试验抗体检测结果的一致性最高。