In this study,genetic stability of foreign genes was checked by using PCR technology. A total of 4 lines of soil samples from transgenic and non-transgenic control Populus alba×P.glandulosa and the soil samples f...In this study,genetic stability of foreign genes was checked by using PCR technology. A total of 4 lines of soil samples from transgenic and non-transgenic control Populus alba×P.glandulosa and the soil samples from the stands were collected in two successive years,and the bacteria,fungi and antinomyces were isolated by using flat dilution method to determine their quantities. The results demonstrated that target genes were stable present in the genome of transgenic poplar. We found that three kinds of soil microorganism appeared different trends within different months and different years. The ANOVA and multiple comparison analyses revealed that there was no significant difference in quantity of the soil microorganisms among most poplar lines at the same time point. Meanwhile,significant differences of the soil microorganism community were found among several transgenic lines,and between a few transgenic and non-transgenic lines,but they did not show any relation to the type and number of foreign genes. Our preliminary results indicated that the 2-year-old transgenic poplar growing in field had no significant effects on soil microorganisms.展开更多
采用硅胶柱色谱等分离方法,对银中杨Populus alba X P.berolinensis L.树叶的化学成分进行分离纯化,依据理化性质及波谱数据分析进行结构鉴定,从中分离鉴定了12个单体化合物,分别为:邻苯二酚(1),苯甲酸(2),水杨醇(3),东莨菪素(4),咖啡酸...采用硅胶柱色谱等分离方法,对银中杨Populus alba X P.berolinensis L.树叶的化学成分进行分离纯化,依据理化性质及波谱数据分析进行结构鉴定,从中分离鉴定了12个单体化合物,分别为:邻苯二酚(1),苯甲酸(2),水杨醇(3),东莨菪素(4),咖啡酸(5),水杨酸(6),2'-苯甲酰水杨苷(7),水杨苷(8),丁二酸(9),特里杨苷(10),3'-苯甲酰水杨苷(11)和6'-苯甲酰水杨苷(12),其中化合物3,4,7,9～12为首次从该植物中分离得到。展开更多
文摘In this study,genetic stability of foreign genes was checked by using PCR technology. A total of 4 lines of soil samples from transgenic and non-transgenic control Populus alba×P.glandulosa and the soil samples from the stands were collected in two successive years,and the bacteria,fungi and antinomyces were isolated by using flat dilution method to determine their quantities. The results demonstrated that target genes were stable present in the genome of transgenic poplar. We found that three kinds of soil microorganism appeared different trends within different months and different years. The ANOVA and multiple comparison analyses revealed that there was no significant difference in quantity of the soil microorganisms among most poplar lines at the same time point. Meanwhile,significant differences of the soil microorganism community were found among several transgenic lines,and between a few transgenic and non-transgenic lines,but they did not show any relation to the type and number of foreign genes. Our preliminary results indicated that the 2-year-old transgenic poplar growing in field had no significant effects on soil microorganisms.
文摘采用硅胶柱色谱等分离方法,对银中杨Populus alba X P.berolinensis L.树叶的化学成分进行分离纯化,依据理化性质及波谱数据分析进行结构鉴定,从中分离鉴定了12个单体化合物,分别为:邻苯二酚(1),苯甲酸(2),水杨醇(3),东莨菪素(4),咖啡酸(5),水杨酸(6),2'-苯甲酰水杨苷(7),水杨苷(8),丁二酸(9),特里杨苷(10),3'-苯甲酰水杨苷(11)和6'-苯甲酰水杨苷(12),其中化合物3,4,7,9～12为首次从该植物中分离得到。
