摘要
【目的】研究生长在3个不同地点的银中杨根和茎中内生细菌和真菌的多样性,为植物和微生物互作研究提供参考。【方法】分别在黑龙江省大庆市林源镇常围子村、齐齐哈尔市错海林场和北京市房山区韩村河东营苗圃,取银中杨根和茎,表面消毒后,提取微生物DNA,通过16S rRNA和内部转录间隔区(ITS)扩增子IlluminaMiSeq测序以确定内生细菌和真菌群落的多样性。【结果】测序结果根据97%的序列相似性水平,将细菌和真菌的reads分别归类为1541和240个OTU。与数据库比对后确定银中杨内生细菌群落主要以放线菌纲、β-变形菌纲、α-变形菌纲、γ-变形菌纲和拟杆菌纲为主,内生真菌群落主要以座囊菌纲、伞菌纲、子囊菌纲、和银耳纲为主。α多样性和β多样性结果表明,北京、大庆和齐齐哈尔3个地点银中杨的茎内生菌群落明显聚集;而根内生菌群落表现出依赖于植物器官和生长环境的现象。Mantel检验结果表明,pH值、土壤有机质(SOM)含量和钾(K)含量与杨树根内生菌群落显著相关(P<0.05);而氮(N)、磷(P)含量并不是解释银中杨根内生菌群落差异的重要因素。由此确定不同生态环境下生长的银中杨不同器官中的核心微生物,共获得23个核心细菌OTU,归属于6个纲;22个核心真菌OTU,归属于7个纲。还可确定7个根内生细菌指示OTU:Actinophytocola、游动放线菌属、假诺卡氏菌属、红微菌属、链霉菌属、贪噬菌属和慢生根瘤菌属;5个茎内生细菌指示OTU:双歧杆菌属、红球菌属、小杆菌属、粪杆菌属和微球菌属。2个根内生真菌指示OTU:小球腔菌属和Ilyonectria;3个茎内生真菌指示OTU:格孢腔目、链格孢属和Endosporium。UpSetR结果表明:内生细菌中有51(3.30%)个OTU被6组样本共有,6个组单独特有的OTU占总OTU数的4.54%~15.44%;内生真菌中有1(0.42%)个OTU被6组样本共有,6个组单独特有的OTU占了总OTU数的2.92%~29.17%。【
【Objective】This paper aims at studying the diversity of endosphere bacterial and fungal microbiome in roots and stems of Populus alba×P.berolinensis grown in three different sites,and the results would provide scientific basis for the study of interactions between plants and microorganisms.【Method】The roots and stems of poplar were collected from three different sites:Changweizi village of Linyuan town in Daqing city,Cuohai farm in Qiqihar city and Hancunhe Dongying nursery in Fangshan District.After surface disinfection,microbial DNA was extracted and sequenced by Illumina MiSeq using 16 s rRNA and internal transcribed spacer amplicon(ITS)amplifiers to determine the bacterial and fungal communities associated with the different plant habitats and niches.【Results】According to the 97%sequence similarity cut-off level,the reads of bacteria and fungi were clustered into 1541 and 240 OTU,respectively.In comparison with the database,we found that Actinobacteria,Betaproteobacteria,Alphaproteobacteria,Gammaproteobacteria and Bacteroidia were the dominant endophytic bacteria,and the fungal community was dominated by Dothideomycetes,Agaricomycetes,Sordariomycetes and Tremellomycetes.The results of alpha and beta diversity showed that the stem endophytic communities of poplar in Beijing,Daqing and Qiqihar were obviously clustered and could not be distinguished.However,the endophytic community of roots was dependent on plant organs and growth environment.The Mantel test results showed that pH,and soil organic matter(SOM)contents and potassium(K)content were significantly correlated with the microbial communities(P<0.05).However,the nitrogen(N)and phosphorus(P)content did not appear to be important factors explaining the variance in the communities of poplar root endophytes.We identified the core microbiome in the different organs of poplar grown in different environmental conditions,and obtained a total of 23 core bacteria OTU belonging to 6 classes,and 22 core fungi OTU belonging to 7 classes,respectively.Spec
作者
王颜波
张伟溪
丁昌俊
苏晓华
Wang Yanbo;Zhang Weixi;Ding Changjun;Su Xiaohua(State Key Laboratory of Tree Genetics and Breeding Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091;Nanchang Institute of Technology ,Nanchang 330099)
出处
《林业科学》
EI
CAS
CSCD
北大核心
2020年第2期48-60,共13页
Scientia Silvae Sinicae
基金
中央级公益性科研院所基本科研业务费专项重点项目子项目“杨树工业用材新型品种选育研究”(CAFYBB2017ZA001-3)
关键词
银中杨
环境条件
内生菌群落
生态位变异
扩增子测序
Populus alba×P.berolinensis
environmental condition
endophytic communities
niche differentiation
amplicon Illumina MiSeq