Epigenetic modifications have been proved to be a powerful way to activate silent gene clusters and lead to diverse secondary metabolites in fungi. Previously, inactivation of a histone H3 deacetylase in Calcarisporiu...Epigenetic modifications have been proved to be a powerful way to activate silent gene clusters and lead to diverse secondary metabolites in fungi. Previously, inactivation of a histone H3 deacetylase in Calcarisporium arbuscula had led to pleiotropic activation and overexpression of more than 75% of the biosynthetic genes and isolation of ten compounds. Further investigation of the crude extract of C. arbuscula Δhda A strain resulted in the isolation of twelve new diterpenoids including three cassanes(1-3), one cleistanthane(4), six pimaranes(5-10), and two isopimaranes(11 and 12) along with two know cleistanthane analogues. Their structures were elucidated by extensive NMR spectroscopic data analysis. Compounds 2 and 4 showed potent inhibitory effects on the expression of MMP1 and MMP2(matrix metalloproteinases family) in human breast cancer(MCF-7) cells.展开更多
A comprehensive chemical study of the endophytic fungus Arthrinium sp. ZS03, associated with Acorus tatarinowii Schott, yielded eleven pimarane diterpenoids (compounds 1–11), including seven novel compounds designate...A comprehensive chemical study of the endophytic fungus Arthrinium sp. ZS03, associated with Acorus tatarinowii Schott, yielded eleven pimarane diterpenoids (compounds 1–11), including seven novel compounds designated arthrinoids A–G (1–7). The determination of their structures and absolute configurations was achieved through extensive spectroscopic techniques, quantum chemical calculations of electronic circular dichroism (ECD), and single-crystal X-ray diffraction analysis. Furthermore, 7 demonstrated inhibitory activity against Klebsiella pneumoniae, comparable to the reference antibiotic amikacin, with a minimum inhibitory concentration (MIC) of 8 μg·mL^(−1).展开更多
The aerial parts of Siegesbeckia have been used as the traditional Chinese medicine in the treatment of rheumatic arthritis, hypertension, malaria, neurasthenia, and snakebite. In order to find new and bioactive compo...The aerial parts of Siegesbeckia have been used as the traditional Chinese medicine in the treatment of rheumatic arthritis, hypertension, malaria, neurasthenia, and snakebite. In order to find new and bioactive compounds, the chemical constituents of the aerial parts of S. orientalis L. were investigated and two new compounds, namely β-D-glucopyranosyl-ent-2-oxo-15,16-dihydroxy-pimar-8（14）-en-19-oic-late （compound 1 ） and [1 （10） E,4Z]-8β-angeloyloxy-9α-methoxy-6α, 15-dihydroxy-14-oxogermacra-1 （10）,4,11 （13）-trien-12-oic acid 12,6-1actone （compound 2）, as well as five known ent-pimarane diterpenes （compounds 3-7） were isolated. The structures of the two new compounds were identified by their physicochemical properties and spectral analysis, particularly oneand two-dimensional nuclear magnetic resonance spectral methods.展开更多
A phytochemical investigation was carried out on the extract of a medicinal plant Callicarpa nudiflora,resulting in the characterization of five new 3,4-seco-isopimarane(1-5)and one new 3,4-seco-pimarane diterpenoid(6...A phytochemical investigation was carried out on the extract of a medicinal plant Callicarpa nudiflora,resulting in the characterization of five new 3,4-seco-isopimarane(1-5)and one new 3,4-seco-pimarane diterpenoid(6),together with four known compounds.The structures of the new compounds were fully elucidated by extensive analysis of MS,1 D and 2 D NMR spectroscopic data,and time-dependent density functional theory(TDDFT)calculation of electronic circular dichroism(ECD)spectra,and DFT calculations for NMR chemical shifts and optical rotations.展开更多
A new pimarane-type diterpenoid,momilactone C(1),together with one known compound,momilactone A(2),was isolated from moss Pseudoleskeella papillosa by column chromatography on silica gel,Sephadex LH-20 and semi-prepar...A new pimarane-type diterpenoid,momilactone C(1),together with one known compound,momilactone A(2),was isolated from moss Pseudoleskeella papillosa by column chromatography on silica gel,Sephadex LH-20 and semi-preparative HPLC.Their structures were established on the basis of HR-ESI-MS,ID and 2D NMR spectroscopic methods.Allelopathic test showed that the two diterpenoids inhibited germination of the Arabidopsis thaliana seeds.展开更多
基金supported financially by National Natural Science Foundation of China (Nos. 21502233 and 81522043)CAMS Initiative for Innovative Medicine (CAMS-I2M-1-010)+1 种基金the PUMC Youth Fund (33320140175)the State Key Laboratory Fund for Excellent Young Scientists to Youcai Hu (GTZB201401)
文摘Epigenetic modifications have been proved to be a powerful way to activate silent gene clusters and lead to diverse secondary metabolites in fungi. Previously, inactivation of a histone H3 deacetylase in Calcarisporium arbuscula had led to pleiotropic activation and overexpression of more than 75% of the biosynthetic genes and isolation of ten compounds. Further investigation of the crude extract of C. arbuscula Δhda A strain resulted in the isolation of twelve new diterpenoids including three cassanes(1-3), one cleistanthane(4), six pimaranes(5-10), and two isopimaranes(11 and 12) along with two know cleistanthane analogues. Their structures were elucidated by extensive NMR spectroscopic data analysis. Compounds 2 and 4 showed potent inhibitory effects on the expression of MMP1 and MMP2(matrix metalloproteinases family) in human breast cancer(MCF-7) cells.
基金supported by the National Natural Science Foundation of China(No.82003956)the Henan Young Elite Scientists Sponsorship Program(No.2023HYTP032)。
文摘A comprehensive chemical study of the endophytic fungus Arthrinium sp. ZS03, associated with Acorus tatarinowii Schott, yielded eleven pimarane diterpenoids (compounds 1–11), including seven novel compounds designated arthrinoids A–G (1–7). The determination of their structures and absolute configurations was achieved through extensive spectroscopic techniques, quantum chemical calculations of electronic circular dichroism (ECD), and single-crystal X-ray diffraction analysis. Furthermore, 7 demonstrated inhibitory activity against Klebsiella pneumoniae, comparable to the reference antibiotic amikacin, with a minimum inhibitory concentration (MIC) of 8 μg·mL^(−1).
文摘The aerial parts of Siegesbeckia have been used as the traditional Chinese medicine in the treatment of rheumatic arthritis, hypertension, malaria, neurasthenia, and snakebite. In order to find new and bioactive compounds, the chemical constituents of the aerial parts of S. orientalis L. were investigated and two new compounds, namely β-D-glucopyranosyl-ent-2-oxo-15,16-dihydroxy-pimar-8（14）-en-19-oic-late （compound 1 ） and [1 （10） E,4Z]-8β-angeloyloxy-9α-methoxy-6α, 15-dihydroxy-14-oxogermacra-1 （10）,4,11 （13）-trien-12-oic acid 12,6-1actone （compound 2）, as well as five known ent-pimarane diterpenes （compounds 3-7） were isolated. The structures of the two new compounds were identified by their physicochemical properties and spectral analysis, particularly oneand two-dimensional nuclear magnetic resonance spectral methods.
基金supported from the China National Key R&D Program(Nos.2017YFE0195100 and 2018YFC.707000)the National Natural Science Foundation of China(Nos.21920102003 and 81903485)。
文摘A phytochemical investigation was carried out on the extract of a medicinal plant Callicarpa nudiflora,resulting in the characterization of five new 3,4-seco-isopimarane(1-5)and one new 3,4-seco-pimarane diterpenoid(6),together with four known compounds.The structures of the new compounds were fully elucidated by extensive analysis of MS,1 D and 2 D NMR spectroscopic data,and time-dependent density functional theory(TDDFT)calculation of electronic circular dichroism(ECD)spectra,and DFT calculations for NMR chemical shifts and optical rotations.
基金The open project of State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences(P2008-KF05)the project of western doctorate foundationthe knowledge innovation project from the northwest institute of plateau biology,Chinese Academy of Sciences
文摘从阴山大戟的根部首次分到一个已知的对映-海松烷型二萜,3α,19-dihydroxyl-ent-pimara-9,15-diene,通过二维波谱方法(1 H-1 H COSY,HMBC,HMQC,ROESY)确定了其结构,并且首次此化合物的H谱和C谱数据进行了全归属.
基金Financial supports from the National Natural Science Foun-dation of China(nos.30730109 and 30925038)are gratefully acknowledged.
文摘A new pimarane-type diterpenoid,momilactone C(1),together with one known compound,momilactone A(2),was isolated from moss Pseudoleskeella papillosa by column chromatography on silica gel,Sephadex LH-20 and semi-preparative HPLC.Their structures were established on the basis of HR-ESI-MS,ID and 2D NMR spectroscopic methods.Allelopathic test showed that the two diterpenoids inhibited germination of the Arabidopsis thaliana seeds.
