Five isolates of pigeon paramyxovirus Ⅰ（PPMV-Ⅰ）,designated as YZ9712,NC9701,NP9904,PB9601 and SZa9803,were isolated from pigeons during epizootics recently in China.The F gene functional domains were amplified by ...Five isolates of pigeon paramyxovirus Ⅰ（PPMV-Ⅰ）,designated as YZ9712,NC9701,NP9904,PB9601 and SZa9803,were isolated from pigeons during epizootics recently in China.The F gene functional domains were amplified by RT-PCR and then cloned into pGEMDR○-T vector and sequenced.A phylogenetic tree based on nucleotide sequence of the F gene segment revealed all the strains were divided into seven genotypes,and five PPMV-Ⅰ isolates similar to strains 1168/84 and 760/83 were grouped into subgenotype Ⅵb.The homology between these five isolates were 98.3%～99.4% and 97.8%～99.4% at the nucleotide and amino acid levels respectively.The deduced amino acid sequence at the cleavage site of the F protein was of virulent strains112K-R-Q-K-R-F117?All the isolates had highly genetic similarity to the England reference strain 1168/84 and Wales reference strain 760/83 of PPMV-Ⅰ,suggesting that they might have originated from a common ancestor.展开更多
为了解广西鸽Ⅰ型副黏病毒(pigeon paramyxovirus type 1,PPMV-1)分离株的基因亚型及遗传进化,2019年从广西鸽场疑似感染PPMV-1的送检临床样品进行病毒分离和鉴定,并对其进行F基因的测序以及系统发育和分子钟的分析。结果显示,从送检样...为了解广西鸽Ⅰ型副黏病毒(pigeon paramyxovirus type 1,PPMV-1)分离株的基因亚型及遗传进化,2019年从广西鸽场疑似感染PPMV-1的送检临床样品进行病毒分离和鉴定,并对其进行F基因的测序以及系统发育和分子钟的分析。结果显示,从送检样品分离3株病毒株,分别命名为Pi/CH/GX1101/2019,Pi/CH/GX1201/2019和Pi/CH/GX1202/2019。测序结果表明其F基因编码区长度均为1662核苷酸(nt),编码553氨基酸(aa)。分离株的F蛋白裂解位点为^(112)RRQKRF^(117),符合强毒株特征;系统发育分析表明3株毒株属于两个基因亚型:Ⅵ.2.1.1.2.1亚型(原Ⅵj)和Ⅵ.2.1.1.2.2亚型(原Ⅵk);分子钟分析结果显示中国Ⅵ.2.1.1.2.1亚型和Ⅵ.2.1.1.2.2亚型毒株大约在20世纪80年代开始出现进化分歧;Ⅵ.2.1.1.2.1亚型的Pi/CH/GX1201/2019和Pi/CH/GX1202/2019与位于进化枝低节点的野鸟源参考毒株W4的亲缘关系较近,同在一个分支中,而Ⅵ.2.1.1.2.2亚型的Pi/CH/GX1101/2019毒株与进化枝低节点的2株野鸟源参考毒株Grey heron/CH/GD/GZ333/2015和European turtle dove/CH/GD/GZ23/2015的亲缘关系相近,同在一个小簇中。研究结果证明广西鸽群主要流行Ⅵ.2.1.1.2.1亚型和Ⅵ.2.1.1.2.2亚型的PPMV-1,并推测存在从野鸟传入商业鸽群的风险。展开更多
文摘Five isolates of pigeon paramyxovirus Ⅰ（PPMV-Ⅰ）,designated as YZ9712,NC9701,NP9904,PB9601 and SZa9803,were isolated from pigeons during epizootics recently in China.The F gene functional domains were amplified by RT-PCR and then cloned into pGEMDR○-T vector and sequenced.A phylogenetic tree based on nucleotide sequence of the F gene segment revealed all the strains were divided into seven genotypes,and five PPMV-Ⅰ isolates similar to strains 1168/84 and 760/83 were grouped into subgenotype Ⅵb.The homology between these five isolates were 98.3%～99.4% and 97.8%～99.4% at the nucleotide and amino acid levels respectively.The deduced amino acid sequence at the cleavage site of the F protein was of virulent strains112K-R-Q-K-R-F117?All the isolates had highly genetic similarity to the England reference strain 1168/84 and Wales reference strain 760/83 of PPMV-Ⅰ,suggesting that they might have originated from a common ancestor.
