AIM: To determine whether the photopic negative response(Ph NR) elicited by transient white flash on white background is characterizing for glaucoma model in rabbits.METHODS: Glaucoma was induced in twelve rabbits by ...AIM: To determine whether the photopic negative response(Ph NR) elicited by transient white flash on white background is characterizing for glaucoma model in rabbits.METHODS: Glaucoma was induced in twelve rabbits by subconjunctival injection of 0.05 m L of betamethasone in right eyes(each 1 m L contain betamethasone dipropionate5 mg and betamethasone sodium phosphate 2 mg).The intraocular pressure(IOP), electroretinogram(ERG) and visual evoked potential(VEP) were measured successively prior and on the 3, 7d, two weeks and four weeks postglaucoma induction. After four weeks, the animals were sacrificed and the globes were histopathologically examined.RESULTS: The IOP increased significantly after one week(P =0.0001), then it gradually returned to the control level. In ERG examination, the means of a and b wave amplitude and latency were not affected significantly.Ph NR amplitude decreased significantly within one week(P =0.0001), but its latency was not affected significantly(P =0.132). The means of VEP latency and amplitude were significantly affected after two weeks and four weeks of glaucoma induction(P =0.0001 and 0.02,respectively). The histopathologic examination of the globes showed reduced number of cells in the retinal ganglion cell layer with multiple vacuoles in the retinal nerve fibre layer.There was significant positive correlation between ganglion cell layer cells and Ph NR amplitude( r =0.8, P =0.002).CONCLUSION: The rise in IOP resulted in irreversible changes or incomplete recovery of VEP and Ph NR amplitude. Both Ph NR and VEP represented good additional tools in early diagnosis of glaucoma.展开更多
背景玻璃体腔注射曲安奈德（TA）对于视网膜中央静脉阻塞（CRVO）患者黄斑区水肿的消退以及视力的维持和提高作用较明显，明视负向反应（PhNR）可以反映视网膜内层视网膜神经节细胞（RGCs）及其轴突的功能，两者间是否存在联系是尚待解...背景玻璃体腔注射曲安奈德（TA）对于视网膜中央静脉阻塞（CRVO）患者黄斑区水肿的消退以及视力的维持和提高作用较明显，明视负向反应（PhNR）可以反映视网膜内层视网膜神经节细胞（RGCs）及其轴突的功能，两者间是否存在联系是尚待解决的问题。目的比较分析CRVO黄斑水肿患者玻璃体腔内注射TA前后视网膜电图（ERG）的PhNR的变化，探讨PhNR作为治疗过程中监测视网膜功能的价值。方法收集比较CRVO伴黄斑水肿者12例13眼，于玻璃体腔内注射TA（0．1ml，4mg）前1d及注射后4周分别用标准小数视力表、光学相干断层扫描仪（OCT）、德国Roland RETI scan3．15系统检查视力、黄斑区视网膜厚度和PhNR。结果接受TA玻璃体腔注射后4周，12例患者13眼中有12眼视力提高，1眼视力不变。与玻璃体腔注射TA前比较，玻璃体腔注射TA后4周OCT显示黄斑区神经上皮细胞层水肿明显减轻，厚度减小；闪光视网膜电图（F—ERG）显示PhNR在玻璃体腔注射TA后波形明显改善。玻璃体腔注射前后患眼的视力分别为0．32±0．12和0．48±0．09，差异有统计学意义（t=6．325，P=0．000）；玻璃体腔注射后患眼黄斑区神经上皮层厚度与注射前比较明显下降[（459．46±131．31）μmvs（297．54±43．31）μm]，差异有统计学意义（t=5．961，P=0．000），玻璃体腔注射前后患眼的PhNR平均振幅值分别为（61．28±20．16）μV和（80．23±22．96）μV，差异有统计学意义（t=4．438，P=0．001）。玻璃体腔注射TA前后黄斑区神经上皮层厚度与PhNR振幅间均无明显的相关性（注射前：r=0．587，P=0．035；注射后：r=一0．011，P=0．971）。结论CRVO黄斑水肿患者行玻穗蚀日奉内沣射TA后，PhNR可以做为监测患者内层视网膜功能变化的有效指标。展开更多
Purpose:.To evaluate the diagnostic performance of the photopic negative response (PhNR) for the detection of primary open-angle glaucoma (POAG). Methods:.Fifty-two normal subjects (52 eyes) and 173 POAG patients.(173...Purpose:.To evaluate the diagnostic performance of the photopic negative response (PhNR) for the detection of primary open-angle glaucoma (POAG). Methods:.Fifty-two normal subjects (52 eyes) and 173 POAG patients.(173 eyes) were studied. The PhNR was elicited using a white stimuli on a white background..The mean deviation (MD) and pattern standard deviation (PSD) of the visual field were measured using standard automated perimetry (SAP)..Spectral domain optical coherence tomography (SD-OCT).was used to measure the mean thickness of the retinal nerve fiber layer (RNFL). Results:.In the glaucoma group,.as compared to the normal group,.the amplitudes of a-waves,.b-waves and PhNR were significantly smaller (P<0.001), and the PhNR implicit time was significantly longer.(P=0.004). The MD, PSD and mean thickness of the RNFL were significantly correlated with the amplitude of the PhNR (P<0.001). The area under the receiver operating characteristic curve (AUCs) for the amplitudes of a-waves,.b-waves and PhNR were 0.853,.0.830 and 0.918, respectively. When the specificity was ≥95%, the sensitivities were 60.4%, 54.2% and 85.4% respectively. Conclusion:The PhNR amplitude was reduced even when the loss in visual field sensitivity was mild, which suggests that PhNR might be a useful indicator of early glaucoma disease.展开更多
文摘AIM: To determine whether the photopic negative response(Ph NR) elicited by transient white flash on white background is characterizing for glaucoma model in rabbits.METHODS: Glaucoma was induced in twelve rabbits by subconjunctival injection of 0.05 m L of betamethasone in right eyes(each 1 m L contain betamethasone dipropionate5 mg and betamethasone sodium phosphate 2 mg).The intraocular pressure(IOP), electroretinogram(ERG) and visual evoked potential(VEP) were measured successively prior and on the 3, 7d, two weeks and four weeks postglaucoma induction. After four weeks, the animals were sacrificed and the globes were histopathologically examined.RESULTS: The IOP increased significantly after one week(P =0.0001), then it gradually returned to the control level. In ERG examination, the means of a and b wave amplitude and latency were not affected significantly.Ph NR amplitude decreased significantly within one week(P =0.0001), but its latency was not affected significantly(P =0.132). The means of VEP latency and amplitude were significantly affected after two weeks and four weeks of glaucoma induction(P =0.0001 and 0.02,respectively). The histopathologic examination of the globes showed reduced number of cells in the retinal ganglion cell layer with multiple vacuoles in the retinal nerve fibre layer.There was significant positive correlation between ganglion cell layer cells and Ph NR amplitude( r =0.8, P =0.002).CONCLUSION: The rise in IOP resulted in irreversible changes or incomplete recovery of VEP and Ph NR amplitude. Both Ph NR and VEP represented good additional tools in early diagnosis of glaucoma.
文摘背景玻璃体腔注射曲安奈德（TA）对于视网膜中央静脉阻塞（CRVO）患者黄斑区水肿的消退以及视力的维持和提高作用较明显，明视负向反应（PhNR）可以反映视网膜内层视网膜神经节细胞（RGCs）及其轴突的功能，两者间是否存在联系是尚待解决的问题。目的比较分析CRVO黄斑水肿患者玻璃体腔内注射TA前后视网膜电图（ERG）的PhNR的变化，探讨PhNR作为治疗过程中监测视网膜功能的价值。方法收集比较CRVO伴黄斑水肿者12例13眼，于玻璃体腔内注射TA（0．1ml，4mg）前1d及注射后4周分别用标准小数视力表、光学相干断层扫描仪（OCT）、德国Roland RETI scan3．15系统检查视力、黄斑区视网膜厚度和PhNR。结果接受TA玻璃体腔注射后4周，12例患者13眼中有12眼视力提高，1眼视力不变。与玻璃体腔注射TA前比较，玻璃体腔注射TA后4周OCT显示黄斑区神经上皮细胞层水肿明显减轻，厚度减小；闪光视网膜电图（F—ERG）显示PhNR在玻璃体腔注射TA后波形明显改善。玻璃体腔注射前后患眼的视力分别为0．32±0．12和0．48±0．09，差异有统计学意义（t=6．325，P=0．000）；玻璃体腔注射后患眼黄斑区神经上皮层厚度与注射前比较明显下降[（459．46±131．31）μmvs（297．54±43．31）μm]，差异有统计学意义（t=5．961，P=0．000），玻璃体腔注射前后患眼的PhNR平均振幅值分别为（61．28±20．16）μV和（80．23±22．96）μV，差异有统计学意义（t=4．438，P=0．001）。玻璃体腔注射TA前后黄斑区神经上皮层厚度与PhNR振幅间均无明显的相关性（注射前：r=0．587，P=0．035；注射后：r=一0．011，P=0．971）。结论CRVO黄斑水肿患者行玻穗蚀日奉内沣射TA后，PhNR可以做为监测患者内层视网膜功能变化的有效指标。
文摘Purpose:.To evaluate the diagnostic performance of the photopic negative response (PhNR) for the detection of primary open-angle glaucoma (POAG). Methods:.Fifty-two normal subjects (52 eyes) and 173 POAG patients.(173 eyes) were studied. The PhNR was elicited using a white stimuli on a white background..The mean deviation (MD) and pattern standard deviation (PSD) of the visual field were measured using standard automated perimetry (SAP)..Spectral domain optical coherence tomography (SD-OCT).was used to measure the mean thickness of the retinal nerve fiber layer (RNFL). Results:.In the glaucoma group,.as compared to the normal group,.the amplitudes of a-waves,.b-waves and PhNR were significantly smaller (P<0.001), and the PhNR implicit time was significantly longer.(P=0.004). The MD, PSD and mean thickness of the RNFL were significantly correlated with the amplitude of the PhNR (P<0.001). The area under the receiver operating characteristic curve (AUCs) for the amplitudes of a-waves,.b-waves and PhNR were 0.853,.0.830 and 0.918, respectively. When the specificity was ≥95%, the sensitivities were 60.4%, 54.2% and 85.4% respectively. Conclusion:The PhNR amplitude was reduced even when the loss in visual field sensitivity was mild, which suggests that PhNR might be a useful indicator of early glaucoma disease.
