目的建立操作便捷、快速、稳定的活体斑马鱼血栓模型。方法研究不同浓度苯肼诱导下斑马鱼血栓形成情况。以2dpf（days post fertilization，dpf,受精后天数）健康AB系野生型斑马鱼作为实验动物，将其暴露于不同浓度（0．5BmoL／L、1gmoL...目的建立操作便捷、快速、稳定的活体斑马鱼血栓模型。方法研究不同浓度苯肼诱导下斑马鱼血栓形成情况。以2dpf（days post fertilization，dpf,受精后天数）健康AB系野生型斑马鱼作为实验动物，将其暴露于不同浓度（0．5BmoL／L、1gmoL／L、2μmoL／L、4μmoL／L、8μmoL／L）的苯肼中，同时设置空白对照组（胚胎养殖用水）和溶剂对照组（0．1％乙醇），处理一段时间后，通过观察斑马鱼尾静脉血流及血栓形成情况进行定性分析，利用邻联茴香胺染色法对心脏红细胞染色强度进行定量分析。结果苯肼处理组（模型组）有血栓形成，与溶剂对照组相比有显著性差异（P〈0．001）。血栓形成率先增高后逐渐减缓，0．5μmoL／L、1μmoL／L血栓形成较少，血栓形成率分别为（19．79±1．55）％、（43．28士1．82）％、2μmoL／L、4μmoL／L、8μmoL／L均诱发较为严重的血栓，血栓形成率分别为（69．85±1．12）％、（70．56±4．60）％和（72．25±2．11）％，三组间没有差异。结论苯肼浓度2μmoL/L为诱发稳定的活体斑马鱼血栓模型的最佳浓度。展开更多
In this work we synthesized new derivatives from Phenyl Hydrazine and series of different Aldehydes (derivatives of benzylidenes). The synthesized compounds contain different aromatic Aldehydes which attached by Benze...In this work we synthesized new derivatives from Phenyl Hydrazine and series of different Aldehydes (derivatives of benzylidenes). The synthesized compounds contain different aromatic Aldehydes which attached by Benzene ring via Hydrazine moiety in glacial acetic acid. These derivatives were characterized by TLC, melting points, Infrared Red, Proton Nuclear Magnetic Resonance, Carbon Thirteen Nuclear Magnetic Resonance and Mass Spectroscopy. Finally, these synthesized derivatives were tested for antiproliferative activity against multiple normal and cancerous cell lines, HepG2 (Liver cancer) and MCF-7 (Breast cancer) cell lines were used for cytotoxic assay.展开更多
In this work, some new oxadiazole derivatives have been prepared, by reacting phenyl hydrazine and acetic anhydride together, which furnished 2,4-dimethyl-4-phenyloxadiazole. This product was reacted with a series of ...In this work, some new oxadiazole derivatives have been prepared, by reacting phenyl hydrazine and acetic anhydride together, which furnished 2,4-dimethyl-4-phenyloxadiazole. This product was reacted with a series of aromatic aldehydes, to obtain a series of oxadiazole derivatives. These derivatives were characterized by TLC, melting points, infrared red, proton nuclear magnetic resonance, carbon thirteen nuclear magnetic resonance and mass spectroscopy. Finally, these synthetized derivatives were tested for antiproliferative activity by two different cell lines. MCF-7 (Breast cancer cell line) and HepG2 (Liver cancer cell line) were used to assess the antiproliferative activity of the prepared compounds.展开更多
The reaction of α-triazolyl-α, β-unsaturated ketones with phenyl hydrazine was studied. The result indicated that cyclization took place and new heterocyclic compounds of substituted 4,5-2H-pyrazoles were obtained,...The reaction of α-triazolyl-α, β-unsaturated ketones with phenyl hydrazine was studied. The result indicated that cyclization took place and new heterocyclic compounds of substituted 4,5-2H-pyrazoles were obtained, whose structures and conformation were characterized by 1H NMR, IR, MS, elemental analysis and X-ray diffraction.展开更多
In order to prevent fire even explosion accident caused by hydrazines in chemical industry,the adiabatic stability of benzoyl hydrazine and p-nitro phenyl hydrazine are tested by Accelerating Rate Calorimeter(ARC).The...In order to prevent fire even explosion accident caused by hydrazines in chemical industry,the adiabatic stability of benzoyl hydrazine and p-nitro phenyl hydrazine are tested by Accelerating Rate Calorimeter(ARC).Then pseudo-inverse matrix method is used to calculate dynamics parameters of the two samples respectively.Finally,the measured data are modified by considering of thermal inertia factor of ARC.The results show that the initial exothermic temperature for benzoyl hydrazine is 141.16℃.with the maximum temperature being 364.50℃ and the time to maxinmm self-heating rate is 1061.11 min.But the initial exothermic temperature for p-Nitro phenyl hydrazine is 88.36℃,with the maximum temperature being 596.38℃ and the time to maximum self-heating rate is 314.67 min.It can be seen that p-nitro phenyl hydrazine is easier to explode and the explosion is more powerful.展开更多
文摘目的建立操作便捷、快速、稳定的活体斑马鱼血栓模型。方法研究不同浓度苯肼诱导下斑马鱼血栓形成情况。以2dpf（days post fertilization，dpf,受精后天数）健康AB系野生型斑马鱼作为实验动物，将其暴露于不同浓度（0．5BmoL／L、1gmoL／L、2μmoL／L、4μmoL／L、8μmoL／L）的苯肼中，同时设置空白对照组（胚胎养殖用水）和溶剂对照组（0．1％乙醇），处理一段时间后，通过观察斑马鱼尾静脉血流及血栓形成情况进行定性分析，利用邻联茴香胺染色法对心脏红细胞染色强度进行定量分析。结果苯肼处理组（模型组）有血栓形成，与溶剂对照组相比有显著性差异（P〈0．001）。血栓形成率先增高后逐渐减缓，0．5μmoL／L、1μmoL／L血栓形成较少，血栓形成率分别为（19．79±1．55）％、（43．28士1．82）％、2μmoL／L、4μmoL／L、8μmoL／L均诱发较为严重的血栓，血栓形成率分别为（69．85±1．12）％、（70．56±4．60）％和（72．25±2．11）％，三组间没有差异。结论苯肼浓度2μmoL/L为诱发稳定的活体斑马鱼血栓模型的最佳浓度。
文摘In this work we synthesized new derivatives from Phenyl Hydrazine and series of different Aldehydes (derivatives of benzylidenes). The synthesized compounds contain different aromatic Aldehydes which attached by Benzene ring via Hydrazine moiety in glacial acetic acid. These derivatives were characterized by TLC, melting points, Infrared Red, Proton Nuclear Magnetic Resonance, Carbon Thirteen Nuclear Magnetic Resonance and Mass Spectroscopy. Finally, these synthesized derivatives were tested for antiproliferative activity against multiple normal and cancerous cell lines, HepG2 (Liver cancer) and MCF-7 (Breast cancer) cell lines were used for cytotoxic assay.
文摘In this work, some new oxadiazole derivatives have been prepared, by reacting phenyl hydrazine and acetic anhydride together, which furnished 2,4-dimethyl-4-phenyloxadiazole. This product was reacted with a series of aromatic aldehydes, to obtain a series of oxadiazole derivatives. These derivatives were characterized by TLC, melting points, infrared red, proton nuclear magnetic resonance, carbon thirteen nuclear magnetic resonance and mass spectroscopy. Finally, these synthetized derivatives were tested for antiproliferative activity by two different cell lines. MCF-7 (Breast cancer cell line) and HepG2 (Liver cancer cell line) were used to assess the antiproliferative activity of the prepared compounds.
文摘The reaction of α-triazolyl-α, β-unsaturated ketones with phenyl hydrazine was studied. The result indicated that cyclization took place and new heterocyclic compounds of substituted 4,5-2H-pyrazoles were obtained, whose structures and conformation were characterized by 1H NMR, IR, MS, elemental analysis and X-ray diffraction.
文摘In order to prevent fire even explosion accident caused by hydrazines in chemical industry,the adiabatic stability of benzoyl hydrazine and p-nitro phenyl hydrazine are tested by Accelerating Rate Calorimeter(ARC).Then pseudo-inverse matrix method is used to calculate dynamics parameters of the two samples respectively.Finally,the measured data are modified by considering of thermal inertia factor of ARC.The results show that the initial exothermic temperature for benzoyl hydrazine is 141.16℃.with the maximum temperature being 364.50℃ and the time to maxinmm self-heating rate is 1061.11 min.But the initial exothermic temperature for p-Nitro phenyl hydrazine is 88.36℃,with the maximum temperature being 596.38℃ and the time to maximum self-heating rate is 314.67 min.It can be seen that p-nitro phenyl hydrazine is easier to explode and the explosion is more powerful.
