A novel artificial site specific cleavage reagent, with peptide nucleic acid （PNA） as sequence-recognizing moiety and cerium （IV） ions as “scissors” for cleaving target DNA, was synthesized. Subsequently, it was...A novel artificial site specific cleavage reagent, with peptide nucleic acid （PNA） as sequence-recognizing moiety and cerium （IV） ions as “scissors” for cleaving target DNA, was synthesized. Subsequently, it was employed in the cleavage of target 26-mer single-stranded DNA （ssDNA）, which has 10-mer sequence complementary with PNA recognizer in the hybrids, under physiological conditions. Reversed-phase high-performance liquid chromatogram （RPHPLC） experiments indicated that the artificial site specific cleavage reagent could cleave the target DNA specifically.展开更多
Protected (L) and (D)-lysine were used respectively as starting materials to synthesize two new types of chiral blocks for the construction of PNA. Nucleobase was linked to alpha-NH2 of lysine via -CH2C (O)- spacer in...Protected (L) and (D)-lysine were used respectively as starting materials to synthesize two new types of chiral blocks for the construction of PNA. Nucleobase was linked to alpha-NH2 of lysine via -CH2C (O)- spacer in type I, and -C (O)- was used in type II. The corresponding oligomers were constructed in solution.展开更多
We prepared the azobenzene PNA unit and synthesized various PNAs containing an azobenzene unit by a typical tBoc method. Both the azobenzene PNA unit and PNA containing an azobenzene unit (N-PNA) showed reversible pho...We prepared the azobenzene PNA unit and synthesized various PNAs containing an azobenzene unit by a typical tBoc method. Both the azobenzene PNA unit and PNA containing an azobenzene unit (N-PNA) showed reversible photoisomerization with UV and visible light irradiation. The hybridization of N-PNA with complementary DNA resulted in a considerable decrease of the photoisomerization rate constant of the azobenzene.展开更多
基金This work was supported by the National Natural Science Foundation of China(No.20443005)the Nanotechnology Special Projects of Shanghai(No.0352nm123)
文摘A novel artificial site specific cleavage reagent, with peptide nucleic acid （PNA） as sequence-recognizing moiety and cerium （IV） ions as “scissors” for cleaving target DNA, was synthesized. Subsequently, it was employed in the cleavage of target 26-mer single-stranded DNA （ssDNA）, which has 10-mer sequence complementary with PNA recognizer in the hybrids, under physiological conditions. Reversed-phase high-performance liquid chromatogram （RPHPLC） experiments indicated that the artificial site specific cleavage reagent could cleave the target DNA specifically.
文摘Protected (L) and (D)-lysine were used respectively as starting materials to synthesize two new types of chiral blocks for the construction of PNA. Nucleobase was linked to alpha-NH2 of lysine via -CH2C (O)- spacer in type I, and -C (O)- was used in type II. The corresponding oligomers were constructed in solution.
基金Supported by the Major State Basic Research Development Program of China (Grant No. 2007CB607605)National Natural Science Foundation of China (Grant No. 20473106)
文摘We prepared the azobenzene PNA unit and synthesized various PNAs containing an azobenzene unit by a typical tBoc method. Both the azobenzene PNA unit and PNA containing an azobenzene unit (N-PNA) showed reversible photoisomerization with UV and visible light irradiation. The hybridization of N-PNA with complementary DNA resulted in a considerable decrease of the photoisomerization rate constant of the azobenzene.
