In order to understand the variation of FPV strains in the Jinan area, Shandong Province, China, the VP2 gene of 6 FPV strains was sequenced, and the analysis of the genetic relationship, evolution and main functional...In order to understand the variation of FPV strains in the Jinan area, Shandong Province, China, the VP2 gene of 6 FPV strains was sequenced, and the analysis of the genetic relationship, evolution and main functional site variation was carried out. It was found that FPV-XY2, FPV-XY3 and FPV-XY6 were the same strain with 100% homology, and also close to FPV-XY1, and the homology between FPV-XY4 and FPV-XY5 was close. The homology between the reference strain and the test strain was over 99.3%. According to the evolutionary analysis, the genetic relationship among FPV-XY1, FPV-XY2, FPV-XY3, FPV-XY6 was close, and the genetic relationship between FPV-XY4 and FPV-XY5 was close, and the result was similar to the homologous result. Compared with the VP2 amino acid sequence of the standard strain FPV-CU4, the VP2 protein of all the test strains changed from I to t on the 101st Amino acid, this may be the cause of immune failure in these 6 cases;the change of a to s in the 91st amino acid position of FPV-XY1, FPV-XY2, FPV-XY3, FPV-XY6 may be the cause of enhanced virulence of FPV. This study provides a reference for exploring the epidemic law of FP in the Jinan area, the standard of FP treatment plan and the research and development of FPV subunit vaccine.展开更多
Feline panleukopenia virus (FPV),belonging to the genus Parvovirus within the family Parvoviridae, infects and causes disease in carnivore species throughout the world. However, few epidemiological studies of this vir...Feline panleukopenia virus (FPV),belonging to the genus Parvovirus within the family Parvoviridae, infects and causes disease in carnivore species throughout the world. However, few epidemiological studies of this virus have been done in wildlife in China. The aims of this study were to determine the infection rate of FPV in captive tigers, lions and domestic cats. Serum samples were obtained from 207 tigers and 4 lions between 2002 and 2006 in different wildlife zoos of China,and 23 domestic cats, and further tested for antibodies against FPV by serum neutralization(SN) assay and hemagglutination-inhibition (HI) assay, whose results are consistent. A seropositive rate of 53.1% was found in tigers,25% in lions and 26% in cats. These results indicated that most of the felines had been infected with feline panleukopenia virus, and the virus may cause disease in carnivore species in China.展开更多
文摘In order to understand the variation of FPV strains in the Jinan area, Shandong Province, China, the VP2 gene of 6 FPV strains was sequenced, and the analysis of the genetic relationship, evolution and main functional site variation was carried out. It was found that FPV-XY2, FPV-XY3 and FPV-XY6 were the same strain with 100% homology, and also close to FPV-XY1, and the homology between FPV-XY4 and FPV-XY5 was close. The homology between the reference strain and the test strain was over 99.3%. According to the evolutionary analysis, the genetic relationship among FPV-XY1, FPV-XY2, FPV-XY3, FPV-XY6 was close, and the genetic relationship between FPV-XY4 and FPV-XY5 was close, and the result was similar to the homologous result. Compared with the VP2 amino acid sequence of the standard strain FPV-CU4, the VP2 protein of all the test strains changed from I to t on the 101st Amino acid, this may be the cause of immune failure in these 6 cases;the change of a to s in the 91st amino acid position of FPV-XY1, FPV-XY2, FPV-XY3, FPV-XY6 may be the cause of enhanced virulence of FPV. This study provides a reference for exploring the epidemic law of FP in the Jinan area, the standard of FP treatment plan and the research and development of FPV subunit vaccine.
文摘Feline panleukopenia virus (FPV),belonging to the genus Parvovirus within the family Parvoviridae, infects and causes disease in carnivore species throughout the world. However, few epidemiological studies of this virus have been done in wildlife in China. The aims of this study were to determine the infection rate of FPV in captive tigers, lions and domestic cats. Serum samples were obtained from 207 tigers and 4 lions between 2002 and 2006 in different wildlife zoos of China,and 23 domestic cats, and further tested for antibodies against FPV by serum neutralization(SN) assay and hemagglutination-inhibition (HI) assay, whose results are consistent. A seropositive rate of 53.1% was found in tigers,25% in lions and 26% in cats. These results indicated that most of the felines had been infected with feline panleukopenia virus, and the virus may cause disease in carnivore species in China.
