Objective To investigate the expression of programmed cell death 5 （PDCD5） in tissues of normal human prostate （NP）, benign prostatic hyperplasia （BPH）, and prostate cancer （PCa） in order to assess the clinica...Objective To investigate the expression of programmed cell death 5 （PDCD5） in tissues of normal human prostate （NP）, benign prostatic hyperplasia （BPH）, and prostate cancer （PCa） in order to assess the clinical role of PDCD5 in PCa. Methods PDCD5 expression was determined by EnVision immunohistochemical staining in forma-lin-fixed and paraffin-embedded specimens obtained from 12 subjects with NP, 22 with BPH, and 22 with PCa. In addition, PCa cases were classified as low/middle-risk （Gleason sumS7） and high-risk （Gleason sum〉7） on the basis of Gleason grade. Positive expression rates and intensity of PDCD5 protein were observed under light microscope and analyzed with computer imaging technique. Expression of PDCD5 was compared among different prostatic tissues. Results The expression of PDCD5 was significantly lower in tissue of PCa than in tissues of NP and BPH （P〈0.01）. However, there was no significant difference in PDCD5 expression between tissues of NP and BPH. In addition, the expression of PDCD5 was further downregulated with the increase of Gleason sum in PCa. Conclusions By downregulating apoptosis, low PDCD5 expression may play an important role in the occurrence and development of PCa. PDCD5 is supposed to have a potential clinical value to be a new predictor of progression and target of gene therapy in PCa.展开更多
本研究观察程序性细胞死亡5基因(programmed cell death 5,PDCD5)重组腺病毒转染K562细胞后对化疗药物依托泊甙的增敏作用。利用AdMaxTM腺病毒载体包装系统,通过同源重组方法构建Ad-PDCD5重组腺病毒及对照腺病毒Ad-null及Ad-eGFP;用不...本研究观察程序性细胞死亡5基因(programmed cell death 5,PDCD5)重组腺病毒转染K562细胞后对化疗药物依托泊甙的增敏作用。利用AdMaxTM腺病毒载体包装系统,通过同源重组方法构建Ad-PDCD5重组腺病毒及对照腺病毒Ad-null及Ad-eGFP;用不同感染复数将Ad-eGFP、Ad-null或Ad-PDCD5转染人白血病细胞系,实时定量PCR检测PDCD5mRNA的相对表达水平;利用MTT法及Annexin-V-FITC/PI双染色流式细胞术观察依托泊甙对转染后K562细胞增殖与凋亡的影响。结果表明:Ad-eGFP腺病毒对白血病细胞系K562、Jurkat及CEM的转染效率可达60%-86%。Ad-PDCD5重组腺病毒能梯度增加K562细胞PDCD5 mRNA的相对表达水平,腺病毒介导的PDCD5基因转移促进依托泊甙诱导的K562细胞凋亡。结论:PDCD5重组腺病毒可能成为化疗药物的增敏剂。展开更多
目的:PDCD5(programmed cell death 5)在多种肿瘤组织和细胞中表达降低,但是在胃癌组织未见系统研究,本文基于数据挖掘分析PDCD5表达对胃癌预后的影响。方法:利用Oncomine数据库挖掘PDCD5在胃癌组织中的表达情况。利用Kaplan-Meier Plot...目的:PDCD5(programmed cell death 5)在多种肿瘤组织和细胞中表达降低,但是在胃癌组织未见系统研究,本文基于数据挖掘分析PDCD5表达对胃癌预后的影响。方法:利用Oncomine数据库挖掘PDCD5在胃癌组织中的表达情况。利用Kaplan-Meier Plotter进行胃癌患者生存周期分析。结果:相比正常胃组织,PDCD5基因在弥漫型胃癌组织和混合型胃癌组织中呈低表达,而肠型胃癌组织未见明显变化。PDCD5高表达能够延长弥漫型胃癌、混合型胃癌和肠型胃癌患者的生存时间。结论:大样本数据挖掘能迅速准确地获取胃癌组织中PDCD5表达的相关信息,为深入研究奠定基础。展开更多
文摘Objective To investigate the expression of programmed cell death 5 （PDCD5） in tissues of normal human prostate （NP）, benign prostatic hyperplasia （BPH）, and prostate cancer （PCa） in order to assess the clinical role of PDCD5 in PCa. Methods PDCD5 expression was determined by EnVision immunohistochemical staining in forma-lin-fixed and paraffin-embedded specimens obtained from 12 subjects with NP, 22 with BPH, and 22 with PCa. In addition, PCa cases were classified as low/middle-risk （Gleason sumS7） and high-risk （Gleason sum〉7） on the basis of Gleason grade. Positive expression rates and intensity of PDCD5 protein were observed under light microscope and analyzed with computer imaging technique. Expression of PDCD5 was compared among different prostatic tissues. Results The expression of PDCD5 was significantly lower in tissue of PCa than in tissues of NP and BPH （P〈0.01）. However, there was no significant difference in PDCD5 expression between tissues of NP and BPH. In addition, the expression of PDCD5 was further downregulated with the increase of Gleason sum in PCa. Conclusions By downregulating apoptosis, low PDCD5 expression may play an important role in the occurrence and development of PCa. PDCD5 is supposed to have a potential clinical value to be a new predictor of progression and target of gene therapy in PCa.
文摘本研究观察程序性细胞死亡5基因(programmed cell death 5,PDCD5)重组腺病毒转染K562细胞后对化疗药物依托泊甙的增敏作用。利用AdMaxTM腺病毒载体包装系统,通过同源重组方法构建Ad-PDCD5重组腺病毒及对照腺病毒Ad-null及Ad-eGFP;用不同感染复数将Ad-eGFP、Ad-null或Ad-PDCD5转染人白血病细胞系,实时定量PCR检测PDCD5mRNA的相对表达水平;利用MTT法及Annexin-V-FITC/PI双染色流式细胞术观察依托泊甙对转染后K562细胞增殖与凋亡的影响。结果表明:Ad-eGFP腺病毒对白血病细胞系K562、Jurkat及CEM的转染效率可达60%-86%。Ad-PDCD5重组腺病毒能梯度增加K562细胞PDCD5 mRNA的相对表达水平,腺病毒介导的PDCD5基因转移促进依托泊甙诱导的K562细胞凋亡。结论:PDCD5重组腺病毒可能成为化疗药物的增敏剂。
文摘目的:PDCD5(programmed cell death 5)在多种肿瘤组织和细胞中表达降低,但是在胃癌组织未见系统研究,本文基于数据挖掘分析PDCD5表达对胃癌预后的影响。方法:利用Oncomine数据库挖掘PDCD5在胃癌组织中的表达情况。利用Kaplan-Meier Plotter进行胃癌患者生存周期分析。结果:相比正常胃组织,PDCD5基因在弥漫型胃癌组织和混合型胃癌组织中呈低表达,而肠型胃癌组织未见明显变化。PDCD5高表达能够延长弥漫型胃癌、混合型胃癌和肠型胃癌患者的生存时间。结论:大样本数据挖掘能迅速准确地获取胃癌组织中PDCD5表达的相关信息,为深入研究奠定基础。
