[Objective] This study was to screen the economic or stable PCR system of rice and detect the generality of the selected system in different molecular markers based on PCR.[Method] With DNA extracted from rice leaves ...[Objective] This study was to screen the economic or stable PCR system of rice and detect the generality of the selected system in different molecular markers based on PCR.[Method] With DNA extracted from rice leaves by CTAB method as the template,PCR system was optimized by L16(45)orthogonal design.[Result] Clear bands were amplified from 16 different combinations,but the amplification effects and yields had difference.The most economic and applicable system was as follows:20 ng DNA template,150 μmol/L dNT...展开更多
文摘目的建立鉴别鱼腥草Houttuynia cordata与百部还魂Gymnotheca chinensis的特异性PCR方法。方法采集不同产地的鱼腥草与百部还魂样品各8份,所有样品提取总DNA。通过对其mat K片段进行扩增、测序,进行同源比对后根据其变异位点设计特异鉴别引物,建立特异PCR鉴别方法,并通过加入SYBR Green I染料法对2种中药进行快速检测。另外,构建多重PCR体系,只经一个PCR反应,就能对鱼腥草与百部还魂进行快速分子鉴定。结果所构建特异PCR与多重PCR体系均能产生鱼腥草185 bp的特异鉴别条带,百部还魂389 bp的特异鉴别条带,SYBR Green I染料可进行快速检测方法。结论建立的鱼腥草与百部还魂2种中药的快速PCR鉴别方法简便、可靠。
基金Supported by the Key Project from Department of Science and Technology of Heilongjiang Province(GA06B102-3-4)the Key Project from Heilongjiang Provincial Reclamation General Administration(HNKXIV-01-01-02)~~
文摘[Objective] This study was to screen the economic or stable PCR system of rice and detect the generality of the selected system in different molecular markers based on PCR.[Method] With DNA extracted from rice leaves by CTAB method as the template,PCR system was optimized by L16(45)orthogonal design.[Result] Clear bands were amplified from 16 different combinations,but the amplification effects and yields had difference.The most economic and applicable system was as follows:20 ng DNA template,150 μmol/L dNT...
