摘要
用4种PCR体系(2×Taq MasterMix,Forward Primer,Reverse Primer,Template DNA,RNase-free Water;2×PCR buffer,2 mmol/L dNTPs,Forward Primer,Reverse Primer,KOD FX,Template DNA,RNase-free Water;10×Bufferfor Blend Taq,Blend Taq,Template,Forward Primer,Reverse Primer,dNTPs,RNase-free Water;10×Taq PCR Buffer,dNTP Mix,Forward Primer,Reverse Primer,MgCl2,Template DNA,Taq DNA Polymerase,RNase-free Water),分别对不同含量和质量的湖北海棠DNA进行PCR实验。结果表明,DNA稀释几倍后对4种PCR体系的影响不大,扩增的条带清晰;但不同质量DNA对4种PCR体系影响很大,低质量DNA只能在混合酶体系下才可以扩增出清晰、明亮的条带,而其他3个体系下的扩增产物均不清晰。
The experiment with four PCR systems（2 ×Taq MasterMix,Forward Primer,Reverse Primer,Template DNA,RNase-free Water;2×PCR buffer,2 mmol/L dNTPs,Forward Primer,Reverse Primer,KOD FX,Template DNA,RNase-free Water;10×Buffer for Blend Taq,Blend Taq,Template,Forward Primer,Reverse Primer,dNTPs,RNase-free Water;10×Taq PCR Buffer,dNTP Mix,Forward Primer,Reverse Primer,MgCl2,Template DNA,Taq DNA Polymerase,RNase-Free Water）,were used respectively to test the different content and quality of Malus hupehensis（Pamp.）Rehd for the PCR experiments.The results showed： the DNA diluted for several times had little influence on the four PCR systems and it could amplilied a clear,bright band,but different quality DNA had a great effect on these four systems.The bad quality DNA could only amplilied a clear,bright band in the mix system and other three systems failed.
出处
《山西农业科学》
2013年第8期771-773,共3页
Journal of Shanxi Agricultural Sciences
基金
国家自然科学基金项目(31070619
31170178)
山东省自然科学基金项目(ZR2011CM045)
