目的探讨经阴道超声三维彩色能量血管成像(transvaginal three-dimensional color power angiography,TV3D-CPA)检测卵巢肿瘤的血管病理学基础,评价TV3D-CPA鉴别卵巢良、恶性肿瘤的临床应用价值及其与临床分期的关系。方法术前应用TV3D-...目的探讨经阴道超声三维彩色能量血管成像(transvaginal three-dimensional color power angiography,TV3D-CPA)检测卵巢肿瘤的血管病理学基础,评价TV3D-CPA鉴别卵巢良、恶性肿瘤的临床应用价值及其与临床分期的关系。方法术前应用TV3D-CPA技术检测61例卵巢肿瘤患者(38例卵巢恶性肿瘤,5例卵巢交界性肿瘤,18例卵巢良性肿瘤),观测肿瘤内血管分布类型,计算肿瘤内血管指数(vascu laryindex,VI)。术后应用抗CD34单克隆抗体对病理切片进行免疫组织化学染色,计数肿瘤微血管密度(micro vessel density,MVD),确定卵巢恶性肿瘤临床分期。分析VI与MVD的相关性及其与临床分期的关系。结果卵巢恶性肿瘤血管分布Ⅲ型30例(78.9%),明显高于良性肿瘤(1例,5.6%),以3D-CPAⅢ型诊断卵巢恶性肿瘤敏感性78.9%,特异性86.9%。卵巢恶性肿瘤VI与MVD均明显高于卵巢良性肿瘤(P<0.05),以VI≥0.035条/cm3诊断卵巢恶性肿瘤敏感性84.2%,特异性70.0%。卵巢恶性肿瘤Ⅲ、Ⅳ期VI及MVD均明显高于Ⅰ、Ⅱ期(P<0.05)。38例卵巢恶性肿瘤VI与MVD呈正相关(r=0.684,P<0.01)。结论TV3D-CPA可术前评价卵巢肿瘤血管的生成,为卵巢良恶性肿瘤的鉴别及肿瘤恶性程度的术前评估提供有价值的信息。展开更多
Objective:To investigate the potential relationships among the ovarian response prediction index(ORPI),follicle-oocyte index(FOI),and clinical pregnancy rate(CPR)in women undergoing their first in vitro fertilization/...Objective:To investigate the potential relationships among the ovarian response prediction index(ORPI),follicle-oocyte index(FOI),and clinical pregnancy rate(CPR)in women undergoing their first in vitro fertilization/intracytoplasmic sperm injectionembryo transfer(IVF/ICSI-ET)fresh cycle transfer.Methods:In this retrospective cohort study,we included 12,218 women who underwent their first IVF/ICSI-ET cycle between December 2014 and January 2021.The primary and secondary outcomes of our study were CPR and cumulative live birth rate(CLBR),respectively.The data were divided into three groups according to the ORPI and FOI tertiles.Multivariate logistic regression analyses,stratification analyses,interaction,restricted cubic splines,and receiver operating characteristic(ROC)curves were constructed to identify the relationships among ORPI,FOI,and CPR.Results:A statistically significant increase in CPR was detected from the lowest to the highest tertile group(ORPI:48.12%,54.07%,and 53.47%,P<0.001;FOI:49.99%,52.95%,and 52.71%,P=0.012).A higher CLBR was observed in the high group(ORPI:38.63%,44.62%,and 44.19%,P<0.001;FOI:41.02%,43.78%,and 42.59%,P=0.039).Multivariate logistic regression analysis revealed no statistically significant differences between ORPI,FOI,and neither CPR(odds ratio[OR][95%confidence interval{CI}],0.99[0.97–1.00]vs.[1.02{0.84–1.24}])nor CLBR(OR[95%CI],0.99[0.97–1.01]vs.0.99[0.81–1.20]).No significant association was found among FOI,ORPI,and CPR,even in the subgroups.Restricted cubic spline analyses indicated the existence of a non-linear relationship across the entire range of FOI and ORPI.The ORPI and FOI variables had poor predictive ability(AUC<0.60)for CPR.Conclusions:Both ORPI and FOI are not reliable predictors of clinical pregnancy or live birth outcomes in fresh ETs.Clinicians and researchers should avoid using FOI and ORPI to assess pregnancy outcomes after fresh ET because of their limited relevance and predictive value.展开更多
Background：Premature ovarian failure （POF） is a disease that affects female fertility but has few effective treatments.Ovarian reserve function plays an important role in female fertility.Recent studies have report...Background：Premature ovarian failure （POF） is a disease that affects female fertility but has few effective treatments.Ovarian reserve function plays an important role in female fertility.Recent studies have reported that hydrogen can protect male fertility.Therefore,we explored the potential protective effect of hydrogen-rich water on ovarian reserve function through a mouse immune POF model.Methods：To set up immune POF model,fifty female BALB/c mice were randomly divided into four groups：Control （mice consumed normal water,n =10）,hydrogen （mice consumed hydrogen-rich water,n =10）,model （mice were immunized with zona pellucida glycoprotein 3 [ZP3] and consumed normal water,n =15）,and model-hydrogen （mice were immunized with ZP3 and consumed hydrogen-rich water,n =15） groups.After 5 weeks,mice were sacrificed.Serum anti-M＆#252;llerian hormone （AMH） levels,granulosa cell （GC） apoptotic index （AI）,B-cell leukemia/lymphoma 2 （Bcl-2）,and BCL2-associated X protein （Bax） expression were examined.Analyses were performed using SPSS 17.0 （SPSS Inc.,Chicago,IL,USA） software.Results：Immune POF model,model group exhibited markedly reduced serum AMH levels compared with those of the control group （5.41 ± 0.91 ng/ml vs.16.23 ± 1.97 ng/ml,P =0.033） and the hydrogen group （19.65 ± 7.82 ng/ml,P =0.006）.The model-hydrogen group displayed significantly higher AMH concentrations compared with that of the model group （15.03 ± 2.75 ng/ml vs.5.41 ± 0.91 ng/ml,P =0.021）.The GC AI was significantly higher in the model group （21.30 ± 1.74%） than those in the control （7.06 ± 0.27%）,hydrogen （5.17 ± 0.41%）,and model-hydrogen groups （11.24 ± 0.58%） （all P 〈 0.001）.The GC AI was significantly higher in the model-hydrogen group compared with that of the hydrogen group （1 1.24 ± 0.58% vs.5.1 7 ± 0.41%,P =0.021）.Compared with those of the model group,ovarian tissue Bcl-2 levels increased （2.18 ± 0.30 vs.3.01 ± 0.33,P =0.045） and the Bax/B展开更多
文摘目的探讨经阴道超声三维彩色能量血管成像(transvaginal three-dimensional color power angiography,TV3D-CPA)检测卵巢肿瘤的血管病理学基础,评价TV3D-CPA鉴别卵巢良、恶性肿瘤的临床应用价值及其与临床分期的关系。方法术前应用TV3D-CPA技术检测61例卵巢肿瘤患者(38例卵巢恶性肿瘤,5例卵巢交界性肿瘤,18例卵巢良性肿瘤),观测肿瘤内血管分布类型,计算肿瘤内血管指数(vascu laryindex,VI)。术后应用抗CD34单克隆抗体对病理切片进行免疫组织化学染色,计数肿瘤微血管密度(micro vessel density,MVD),确定卵巢恶性肿瘤临床分期。分析VI与MVD的相关性及其与临床分期的关系。结果卵巢恶性肿瘤血管分布Ⅲ型30例(78.9%),明显高于良性肿瘤(1例,5.6%),以3D-CPAⅢ型诊断卵巢恶性肿瘤敏感性78.9%,特异性86.9%。卵巢恶性肿瘤VI与MVD均明显高于卵巢良性肿瘤(P<0.05),以VI≥0.035条/cm3诊断卵巢恶性肿瘤敏感性84.2%,特异性70.0%。卵巢恶性肿瘤Ⅲ、Ⅳ期VI及MVD均明显高于Ⅰ、Ⅱ期(P<0.05)。38例卵巢恶性肿瘤VI与MVD呈正相关(r=0.684,P<0.01)。结论TV3D-CPA可术前评价卵巢肿瘤血管的生成,为卵巢良恶性肿瘤的鉴别及肿瘤恶性程度的术前评估提供有价值的信息。
文摘Objective:To investigate the potential relationships among the ovarian response prediction index(ORPI),follicle-oocyte index(FOI),and clinical pregnancy rate(CPR)in women undergoing their first in vitro fertilization/intracytoplasmic sperm injectionembryo transfer(IVF/ICSI-ET)fresh cycle transfer.Methods:In this retrospective cohort study,we included 12,218 women who underwent their first IVF/ICSI-ET cycle between December 2014 and January 2021.The primary and secondary outcomes of our study were CPR and cumulative live birth rate(CLBR),respectively.The data were divided into three groups according to the ORPI and FOI tertiles.Multivariate logistic regression analyses,stratification analyses,interaction,restricted cubic splines,and receiver operating characteristic(ROC)curves were constructed to identify the relationships among ORPI,FOI,and CPR.Results:A statistically significant increase in CPR was detected from the lowest to the highest tertile group(ORPI:48.12%,54.07%,and 53.47%,P<0.001;FOI:49.99%,52.95%,and 52.71%,P=0.012).A higher CLBR was observed in the high group(ORPI:38.63%,44.62%,and 44.19%,P<0.001;FOI:41.02%,43.78%,and 42.59%,P=0.039).Multivariate logistic regression analysis revealed no statistically significant differences between ORPI,FOI,and neither CPR(odds ratio[OR][95%confidence interval{CI}],0.99[0.97–1.00]vs.[1.02{0.84–1.24}])nor CLBR(OR[95%CI],0.99[0.97–1.01]vs.0.99[0.81–1.20]).No significant association was found among FOI,ORPI,and CPR,even in the subgroups.Restricted cubic spline analyses indicated the existence of a non-linear relationship across the entire range of FOI and ORPI.The ORPI and FOI variables had poor predictive ability(AUC<0.60)for CPR.Conclusions:Both ORPI and FOI are not reliable predictors of clinical pregnancy or live birth outcomes in fresh ETs.Clinicians and researchers should avoid using FOI and ORPI to assess pregnancy outcomes after fresh ET because of their limited relevance and predictive value.
文摘Background：Premature ovarian failure （POF） is a disease that affects female fertility but has few effective treatments.Ovarian reserve function plays an important role in female fertility.Recent studies have reported that hydrogen can protect male fertility.Therefore,we explored the potential protective effect of hydrogen-rich water on ovarian reserve function through a mouse immune POF model.Methods：To set up immune POF model,fifty female BALB/c mice were randomly divided into four groups：Control （mice consumed normal water,n =10）,hydrogen （mice consumed hydrogen-rich water,n =10）,model （mice were immunized with zona pellucida glycoprotein 3 [ZP3] and consumed normal water,n =15）,and model-hydrogen （mice were immunized with ZP3 and consumed hydrogen-rich water,n =15） groups.After 5 weeks,mice were sacrificed.Serum anti-M＆#252;llerian hormone （AMH） levels,granulosa cell （GC） apoptotic index （AI）,B-cell leukemia/lymphoma 2 （Bcl-2）,and BCL2-associated X protein （Bax） expression were examined.Analyses were performed using SPSS 17.0 （SPSS Inc.,Chicago,IL,USA） software.Results：Immune POF model,model group exhibited markedly reduced serum AMH levels compared with those of the control group （5.41 ± 0.91 ng/ml vs.16.23 ± 1.97 ng/ml,P =0.033） and the hydrogen group （19.65 ± 7.82 ng/ml,P =0.006）.The model-hydrogen group displayed significantly higher AMH concentrations compared with that of the model group （15.03 ± 2.75 ng/ml vs.5.41 ± 0.91 ng/ml,P =0.021）.The GC AI was significantly higher in the model group （21.30 ± 1.74%） than those in the control （7.06 ± 0.27%）,hydrogen （5.17 ± 0.41%）,and model-hydrogen groups （11.24 ± 0.58%） （all P 〈 0.001）.The GC AI was significantly higher in the model-hydrogen group compared with that of the hydrogen group （1 1.24 ± 0.58% vs.5.1 7 ± 0.41%,P =0.021）.Compared with those of the model group,ovarian tissue Bcl-2 levels increased （2.18 ± 0.30 vs.3.01 ± 0.33,P =0.045） and the Bax/B
