Dear Editor,In most flowering plants, the stigma of the flower represents the barrier that prevents any unrelated or incompatible （genetically similar） pollen from germination and fertilizing the ovule, leading to e...Dear Editor,In most flowering plants, the stigma of the flower represents the barrier that prevents any unrelated or incompatible （genetically similar） pollen from germination and fertilizing the ovule, leading to either defective or genetically unfavorable embryos. In Brassicaceae, self-incompatibility （SI） is a genetic mechanism in the stigmas that can discern compatible versus incompatible mate （pollen）, in turn, rejecting self or incompatible pollen, while allowing non-self or compatible pollen to develop successfully to fertilize the ovules. Thus, stigmas have evolved complex mechanisms and specialized proteins to reject incompatible mates and to recognize and accept compatible pollen （Doucet et al., 2016）. The fact that SI has been shown to exclusively operate in the stigmas temporally before and during anthesis （Kandasamy et al., 1993） indicates that multiple metabolic pathways essential for pollination exist in these stigmatic papillary cells.展开更多
十字花科作物主要由S位点的复等位基因控制自交不亲和性,羽衣甘蓝(Brassica oleracea var. acephala)是十字花科芸薹属中典型的自交不亲和性植物。本研究利用20对SSR引物首次构建了20个羽衣甘蓝自交系的指纹图谱,20对SSR引物共鉴定到65...十字花科作物主要由S位点的复等位基因控制自交不亲和性,羽衣甘蓝(Brassica oleracea var. acephala)是十字花科芸薹属中典型的自交不亲和性植物。本研究利用20对SSR引物首次构建了20个羽衣甘蓝自交系的指纹图谱,20对SSR引物共鉴定到65个等位变异,平均每对引物含有3.25个变异。等权重算术平均聚类法(UPGMA)将20个自交系聚类为3个遗传类群:OK1、OK2和OK3。其中遗传类群OK1的叶形为紧凑皱叶或披散皱叶,波浪状叶缘,叶色以红紫色为主;遗传类群OK2的叶形为光滑叶,波浪状或裂叶叶缘,叶色为白色外叶;遗传类群OK3的叶形为光滑叶,波浪状或裂叶叶缘,叶色以红紫色为主。利用SRK基因激酶区的特异性引物分析发现,20个羽衣甘蓝自交系中共有9个SRK单元型,分别是8个ClassⅠ型SRK单元型(SRK7、SRK13、SRK14、SRK16、SRK23、SRK36、SRK45和SRK51)和1个ClassⅡ型SRK单元型(SRK15)。遗传类群OK1主要为SRK15单元型(50%),其次为SRK23单元型(20%);遗传类群OK2主要为SRK15、SRK16单元型(均为40%);遗传类群OK3主要为SRK23单元型(40%)。亲和指数测定结果进一步验证了不同遗传类群内部或遗传类群间自交系杂交的亲和关系,杂交组合配制的过程中应兼顾遗传类群和SRK单元型。展开更多
文摘Dear Editor,In most flowering plants, the stigma of the flower represents the barrier that prevents any unrelated or incompatible （genetically similar） pollen from germination and fertilizing the ovule, leading to either defective or genetically unfavorable embryos. In Brassicaceae, self-incompatibility （SI） is a genetic mechanism in the stigmas that can discern compatible versus incompatible mate （pollen）, in turn, rejecting self or incompatible pollen, while allowing non-self or compatible pollen to develop successfully to fertilize the ovules. Thus, stigmas have evolved complex mechanisms and specialized proteins to reject incompatible mates and to recognize and accept compatible pollen （Doucet et al., 2016）. The fact that SI has been shown to exclusively operate in the stigmas temporally before and during anthesis （Kandasamy et al., 1993） indicates that multiple metabolic pathways essential for pollination exist in these stigmatic papillary cells.
文摘十字花科作物主要由S位点的复等位基因控制自交不亲和性,羽衣甘蓝(Brassica oleracea var. acephala)是十字花科芸薹属中典型的自交不亲和性植物。本研究利用20对SSR引物首次构建了20个羽衣甘蓝自交系的指纹图谱,20对SSR引物共鉴定到65个等位变异,平均每对引物含有3.25个变异。等权重算术平均聚类法(UPGMA)将20个自交系聚类为3个遗传类群:OK1、OK2和OK3。其中遗传类群OK1的叶形为紧凑皱叶或披散皱叶,波浪状叶缘,叶色以红紫色为主;遗传类群OK2的叶形为光滑叶,波浪状或裂叶叶缘,叶色为白色外叶;遗传类群OK3的叶形为光滑叶,波浪状或裂叶叶缘,叶色以红紫色为主。利用SRK基因激酶区的特异性引物分析发现,20个羽衣甘蓝自交系中共有9个SRK单元型,分别是8个ClassⅠ型SRK单元型(SRK7、SRK13、SRK14、SRK16、SRK23、SRK36、SRK45和SRK51)和1个ClassⅡ型SRK单元型(SRK15)。遗传类群OK1主要为SRK15单元型(50%),其次为SRK23单元型(20%);遗传类群OK2主要为SRK15、SRK16单元型(均为40%);遗传类群OK3主要为SRK23单元型(40%)。亲和指数测定结果进一步验证了不同遗传类群内部或遗传类群间自交系杂交的亲和关系,杂交组合配制的过程中应兼顾遗传类群和SRK单元型。
