目的探讨如何利用多参数流式细胞术(MFC)鉴别与急性髓系白血病(AML)微小残留病(MRD)具有类似表型的正常细胞。方法回顾性分析2020年3月-2022年4月在我院利用MFC检测的AML患者MRD骨髓标本157例次,识别容易被误认为是MRD的正常细胞。结果...目的探讨如何利用多参数流式细胞术(MFC)鉴别与急性髓系白血病(AML)微小残留病(MRD)具有类似表型的正常细胞。方法回顾性分析2020年3月-2022年4月在我院利用MFC检测的AML患者MRD骨髓标本157例次,识别容易被误认为是MRD的正常细胞。结果AML患者治疗后再生的骨髓样本中会出现如下易误判为MRD的正常细胞群:CD117 dim CD56^(+)CD7^(+)CD45^(str)自然杀伤(NK)细胞、CD19 dim CD56^(+)CD7^(+)CD45^(str) NK细胞、CD300e^(+)HLA-DR^(+)CD14^(part) CD64^(part)非经典单核细胞;这些细胞群占骨髓有核细胞比例的中位值分别是0.084%(范围:0~0.6200%)、0%(范围:0~0.2134%)、0.1549%(范围:0~2.0940%)。结论MFC检测AML患者MRD过程中,应避免将治疗后骨髓再生的正常细胞,误判为残留病。展开更多
Purpose: Exposure to a particular pattern of weak (~3 to 5 μT) magnetic fields produced by computer-generated point durations within three-dimensions completely dissolved malignant cancer cells but not healthy cells....Purpose: Exposure to a particular pattern of weak (~3 to 5 μT) magnetic fields produced by computer-generated point durations within three-dimensions completely dissolved malignant cancer cells but not healthy cells. Biomolecular analyses and confocal microscopy indicated excessive expansion followed by contraction contributed to the “explosion” of the cell. However, after months of replicable effects, the phenomenon slowly ceased. Considering the potency of the complete dissolution of cancer cell lines after 5 days of 6.5-hour daily exposures and the implications for human treatment, the potential source of the disappearance of the effect was pursued by summarizing all of the 50 experiments and assessing the likely etiologies. Materials and Methods: B16-BL6, MDAMB 231 and MCF7 malignant cells and HSG, a non-malignant cell line, were exposed to a sham-field condition or to a specific pattern of computer-generated magnetic fields produced from converting different voltages, each with point durations of 3 ms to 3-D magnetic fields. Conclusion: The specific serial presentation of the two field patterns (one frequency modulated;the other amplitude and frequency modulated) completely dissolved malignant cells but not normal cells within a “zone” within the exposure volume at the conjunction of the three planes of the applied magnetic fields. The affected cells underwent massive melanin production, expansion, contraction and “beading” of submembrane actin structures before fragmentation within this zone. However, this powerful all-or-none phenomenon may have been disrupted by moving the cells, excess mechanical agitation during exposure, or non-optimal point durations of the field parameters. Indirect effects from communication signals (WIFI) through line currents that operated the incubators could not be excluded.展开更多
目的探究慢性下肢静脉曲张性溃疡(Chronic varicose ulcer of lower extrenity,CLU)感染对凝血指标及调节活化正常T细胞表达和趋化因子(Regulating the expression of activated normal T cells and chemokines,RANTES)表达的影响。方...目的探究慢性下肢静脉曲张性溃疡(Chronic varicose ulcer of lower extrenity,CLU)感染对凝血指标及调节活化正常T细胞表达和趋化因子(Regulating the expression of activated normal T cells and chemokines,RANTES)表达的影响。方法选择2017年1月-2019年1月于常州市第二人民医院确诊的原发性下肢静脉曲张的住院治疗患者120例为研究对象,根据是否发生溃疡以及患肢渗出物的细菌培养结果,分为下肢静脉曲张组51例(CVI组)、仅合并溃疡者40例(CLU组)、CLU合并感染者29例(CLU合并感染组)。采用电阻法检测血小板参数[血小板数(PLT)、血小板体积分布宽度(PDW)、血小板平均体积(MPV)]。采用凝固法检测凝血功能指标[纤维蛋白原(FIB)、凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)]。采用逆转录-聚合酶链反应(RT-PCR)二步法检测RANTES mRNA表达情况。采用酶联免疫吸附(ELISA)法检测血清炎性因子[白细胞介素-6(IL-6)和IL-10]。结果CLU合并感染组的PLT为(291.28±43.57)×10^9/L高于CVI组(205.31±40.22)×10^9/L和CLU组(246.35±41.24)×10^9/L;而MPV为(6.35±0.69)fl低于CVI组(8.23±0.81)fl和CLU组(7.16±0.76)fl(P<0.001);CLU合并感染组的FIB为(4.71±0.89)g/L高于CVI组(3.12±0.73)g/L和CLU组(3.89±0.81)g/L;而PT为(11.98±0.52)s低于CVI组(13.23±0.62)s和CLU组(12.40±0.58)s(P<0.001);CLU合并感染组的RANTES mRNA表达水平为(2.59±0.30)高于CVI组和CLU组(P<0.001);CLU合并感染组的血清IL-10为(125.35±29.84)pg/ml低于CVI组和CLU组;而IL-6为(40.12±7.38)pg/ml高于CVI组和CLU组(P<0.001)。结论慢性下肢静脉曲张性溃疡患者血液呈高凝状态,感染会进一步加重血液状态,增加静脉血栓风险。RANTES高表达和炎性因子IL-6、IL-10水平变化可能与溃疡形成和感染有关。展开更多
文摘目的探讨如何利用多参数流式细胞术(MFC)鉴别与急性髓系白血病(AML)微小残留病(MRD)具有类似表型的正常细胞。方法回顾性分析2020年3月-2022年4月在我院利用MFC检测的AML患者MRD骨髓标本157例次,识别容易被误认为是MRD的正常细胞。结果AML患者治疗后再生的骨髓样本中会出现如下易误判为MRD的正常细胞群:CD117 dim CD56^(+)CD7^(+)CD45^(str)自然杀伤(NK)细胞、CD19 dim CD56^(+)CD7^(+)CD45^(str) NK细胞、CD300e^(+)HLA-DR^(+)CD14^(part) CD64^(part)非经典单核细胞;这些细胞群占骨髓有核细胞比例的中位值分别是0.084%(范围:0~0.6200%)、0%(范围:0~0.2134%)、0.1549%(范围:0~2.0940%)。结论MFC检测AML患者MRD过程中,应避免将治疗后骨髓再生的正常细胞,误判为残留病。
文摘Purpose: Exposure to a particular pattern of weak (~3 to 5 μT) magnetic fields produced by computer-generated point durations within three-dimensions completely dissolved malignant cancer cells but not healthy cells. Biomolecular analyses and confocal microscopy indicated excessive expansion followed by contraction contributed to the “explosion” of the cell. However, after months of replicable effects, the phenomenon slowly ceased. Considering the potency of the complete dissolution of cancer cell lines after 5 days of 6.5-hour daily exposures and the implications for human treatment, the potential source of the disappearance of the effect was pursued by summarizing all of the 50 experiments and assessing the likely etiologies. Materials and Methods: B16-BL6, MDAMB 231 and MCF7 malignant cells and HSG, a non-malignant cell line, were exposed to a sham-field condition or to a specific pattern of computer-generated magnetic fields produced from converting different voltages, each with point durations of 3 ms to 3-D magnetic fields. Conclusion: The specific serial presentation of the two field patterns (one frequency modulated;the other amplitude and frequency modulated) completely dissolved malignant cells but not normal cells within a “zone” within the exposure volume at the conjunction of the three planes of the applied magnetic fields. The affected cells underwent massive melanin production, expansion, contraction and “beading” of submembrane actin structures before fragmentation within this zone. However, this powerful all-or-none phenomenon may have been disrupted by moving the cells, excess mechanical agitation during exposure, or non-optimal point durations of the field parameters. Indirect effects from communication signals (WIFI) through line currents that operated the incubators could not be excluded.
文摘目的探究慢性下肢静脉曲张性溃疡(Chronic varicose ulcer of lower extrenity,CLU)感染对凝血指标及调节活化正常T细胞表达和趋化因子(Regulating the expression of activated normal T cells and chemokines,RANTES)表达的影响。方法选择2017年1月-2019年1月于常州市第二人民医院确诊的原发性下肢静脉曲张的住院治疗患者120例为研究对象,根据是否发生溃疡以及患肢渗出物的细菌培养结果,分为下肢静脉曲张组51例(CVI组)、仅合并溃疡者40例(CLU组)、CLU合并感染者29例(CLU合并感染组)。采用电阻法检测血小板参数[血小板数(PLT)、血小板体积分布宽度(PDW)、血小板平均体积(MPV)]。采用凝固法检测凝血功能指标[纤维蛋白原(FIB)、凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)]。采用逆转录-聚合酶链反应(RT-PCR)二步法检测RANTES mRNA表达情况。采用酶联免疫吸附(ELISA)法检测血清炎性因子[白细胞介素-6(IL-6)和IL-10]。结果CLU合并感染组的PLT为(291.28±43.57)×10^9/L高于CVI组(205.31±40.22)×10^9/L和CLU组(246.35±41.24)×10^9/L;而MPV为(6.35±0.69)fl低于CVI组(8.23±0.81)fl和CLU组(7.16±0.76)fl(P<0.001);CLU合并感染组的FIB为(4.71±0.89)g/L高于CVI组(3.12±0.73)g/L和CLU组(3.89±0.81)g/L;而PT为(11.98±0.52)s低于CVI组(13.23±0.62)s和CLU组(12.40±0.58)s(P<0.001);CLU合并感染组的RANTES mRNA表达水平为(2.59±0.30)高于CVI组和CLU组(P<0.001);CLU合并感染组的血清IL-10为(125.35±29.84)pg/ml低于CVI组和CLU组;而IL-6为(40.12±7.38)pg/ml高于CVI组和CLU组(P<0.001)。结论慢性下肢静脉曲张性溃疡患者血液呈高凝状态,感染会进一步加重血液状态,增加静脉血栓风险。RANTES高表达和炎性因子IL-6、IL-10水平变化可能与溃疡形成和感染有关。
