摘要
采用白血病祖细胞集落形成（ＣＦＵＡＭＬ） 培养法，观察不同浓度人参总皂甙（ＴＳＰＧ） 对ＨＬ６０、Ｍｅｇ０１ 白血病细胞株和急性髓细胞白血病患者的骨髓细胞集落形成的影响，并以正常粒单祖细胞（ＣＦＵＧＭ）作比较。结果：正常和白血病祖细胞均对低浓度（５～２５ｕｇ／ｍｌ） 的ＴＳＰＧ有反应，集落数提高２６．１～３５．４％ ；中等浓度（７５ｕｇ／ｍｌ） 的ＴＳＰＧ仍然刺激正常ＣＦＵＧＭ 集落增加，但抑制ＨＬ６０、Ｍｅｇ０１ 和ＣＦＵＡＭＬ集落生长，抑制率分别为５０．８％ 、２５ ．１ ％ 和１９．０％ ；较高浓度（１００ｕｇ／ｍｌ） 的ＴＳＰＧ对白血病祖细胞的抑制作用更明显，ＨＬ６０ 不能形成集落，对Ｍｅｇ０１ 和ＣＦＵＡＭＬ的抑制率分别为５３．３％ 和２８ ．９ ％ ，而正常ＣＦＵＧＭ 仍生长良好。提示ＴＳＰＧ对白血病祖细胞的影响不同于正常祖细胞。
Colony forming culture method of leukemic ancestral cells was used to observe influence of total saponin of ginseng(TSPG)in different concentration to colony formation of HL-60.Meg-01 leukemic clone and marrow cell(CFU-AML)in the patients with acute myelocytic leukemia and also compared with normal granular ancestral cells(CFU-GM).The results showed that both normal and leukemic ancestral cells reacted to TSPG in the lower concentration(5～25ug/ml)and colony unit enhanced to 26.1～35.4%.TSPG in the medium concentration(75ug/ml)still stimulated increase in normal CFU-GM colony but inhibited colony growth of HL-60,Meg-01 and CFU-AMI.The inhibitory rates were respectively 50.8%,25.1% and 19.0%.The inhibitory function of TSPG in the higher concentration(100ug/ml)to leukemic ancestral cells was more obvious and its inhibitory rates to Meg-01 and CFU-AML were respectively 53.3% and 28.9%.But,normal CFU-GM still grew quite well.These results indicate that influence of TSPG to leukemic ancestral cell is different from its influence to normal ancestral cells and TSPG in different concentration shows either the proliferative or inhibitory functions.
出处
《上海中医药杂志》
北大核心
1999年第9期46-48,共3页
Shanghai Journal of Traditional Chinese Medicine
基金
浙江省自然科学基金
澳大利亚政府科研基金
关键词
人参总皂甙
白血病祖细胞
中药
药理
Total saponin of ginseng(TSPG),leukemic ancesttal cells(HL-60,Meg-01,CFU-AML),normal granular ancestral cells(CFU-GM),proliferation,inhibition
