In vertebrates, non-lens βγ-crystallins are widely expressed in various tissues and their functions are not well known. The molecular mechanisms of trefoil factors （TFFs）, which involved in mucosal healing and tum...In vertebrates, non-lens βγ-crystallins are widely expressed in various tissues and their functions are not well known. The molecular mechanisms of trefoil factors （TFFs）, which involved in mucosal healing and tumorigenesis, have remained elusive. βγ-CAT is a novel multifunctional protein complex of non-lens βγ-crystallin and trefoil factor from frog skin secretions. Here we report that βγ-CAT could induce sustained contraction of isolated rabbit aortic rings in dosage （2-35nmol/L） and endothelium dependent manners （P〈0.01 ）. In addition, in situ immunofluorescence indicated that positive TNF-α signals were mainly detected at the endothelial cell layer of βγ-CAT （25nmol/L） treated rings. Furthermore, βγ-CAT induced primary cultured rabbit thoracic aortic endothelial cells （RAECs） rapidly to release TNF-α. After βγ-CAT （25nmol/L） treated for 10 and 30min, the levels of the endothelial cells released TNF-ct were 34.17±5.10 pg/mL and 98.01±4.67 pg/mL （P〈0.01）, respectively. In conclusion, βγ-CAT could induce sustained contraction of isolated aortic rings, and the contractile effect might be partially explained by the release of TNF-α. These findings will give new insight into understanding the functions and physiological roles of non-lens βγ-crystallins and trefoil factors.展开更多
In order to resolve the problems of machining non-axisymmetric aspheric lens,which is short of flexibility in mould grinding and needs high accuracy CNC machine center in globediamond wheel grinding, a new parallel gr...In order to resolve the problems of machining non-axisymmetric aspheric lens,which is short of flexibility in mould grinding and needs high accuracy CNC machine center in globediamond wheel grinding, a new parallel grinding method that utilizes common arc diamond wheel isput forward. Base on the approach calculation of machining locus, the advantages of parallelgrinding that machines non-axisymmetric aspheric lens by 2.5-axis CNC machine center have beenobtained. The results of grinding experiment show the new method can meet the need of grinding highaccuracy non-axisymmetric aspheric lens.展开更多
AIM： To compare characteristics of preloaded and nonpreloaded intraocular lens（IOL） delivery systems during IOL delivery procedures. METHODS： Total 101 human eyes were included in this prospective observational ca...AIM： To compare characteristics of preloaded and nonpreloaded intraocular lens（IOL） delivery systems during IOL delivery procedures. METHODS： Total 101 human eyes were included in this prospective observational case series. Delivery characteristics of 5 types of IOLs including i Sert250 NC60（NC60）, En Vista MX60（MX60）, Acry Sof IQ SN60 WF（SN60 WF）, TECNIS ZCB00（ZCB00）, and TECNIS PCB00（PCB00） were investigated. NC60 and PCB00 were injected via preloaded delivery systems and other IOLs were injected via nonpreloaded systems. In the human trial, time taken from IOL loading to completion of implantation was measured in all eyes undergoing conventional cataract surgery. Using 4 excised porcine eyes, dynamics of ophthalmic viscosurgical device（OVD） between an IOL injector and a porcine eye was analyzed using fluorescein sodiumstained OVD. RESULTS： The average time for IOL implantation was 22.0 s for NC60, 43.2 s for MX60, 32.3 s for SN60 WF, 41.4 s for ZCB00, and 14.6 s for PCB00 respectively. The number of cases with IOL manipulation with a second instrument was 6 for MX60, 2 for ZCB00, 0 for SN60 WF, NC60, and PCB00. Amount of OVD pushed into a porcine eye was smaller with a preloaded system than with non-preloaded systems. CONCLUSION： IOL delivery with preloaded systems is faster and more predictable. Moreover, a preloaded delivery system shows relatively less OVD pushed into a porcine eye than non-preloaded systems.展开更多
基金National Natural Science Foundation (30630014, 30570359)The grant of "Key Research Direction-KSCX2-YW-R-088" from Chinese Academy of Sciences~~
文摘In vertebrates, non-lens βγ-crystallins are widely expressed in various tissues and their functions are not well known. The molecular mechanisms of trefoil factors （TFFs）, which involved in mucosal healing and tumorigenesis, have remained elusive. βγ-CAT is a novel multifunctional protein complex of non-lens βγ-crystallin and trefoil factor from frog skin secretions. Here we report that βγ-CAT could induce sustained contraction of isolated rabbit aortic rings in dosage （2-35nmol/L） and endothelium dependent manners （P〈0.01 ）. In addition, in situ immunofluorescence indicated that positive TNF-α signals were mainly detected at the endothelial cell layer of βγ-CAT （25nmol/L） treated rings. Furthermore, βγ-CAT induced primary cultured rabbit thoracic aortic endothelial cells （RAECs） rapidly to release TNF-α. After βγ-CAT （25nmol/L） treated for 10 and 30min, the levels of the endothelial cells released TNF-ct were 34.17±5.10 pg/mL and 98.01±4.67 pg/mL （P〈0.01）, respectively. In conclusion, βγ-CAT could induce sustained contraction of isolated aortic rings, and the contractile effect might be partially explained by the release of TNF-α. These findings will give new insight into understanding the functions and physiological roles of non-lens βγ-crystallins and trefoil factors.
基金This project is supported by Provincial Foundation for Young Scientists & Scholars Innovation of Fujian, China (No.0013K13004).
文摘In order to resolve the problems of machining non-axisymmetric aspheric lens,which is short of flexibility in mould grinding and needs high accuracy CNC machine center in globediamond wheel grinding, a new parallel grinding method that utilizes common arc diamond wheel isput forward. Base on the approach calculation of machining locus, the advantages of parallelgrinding that machines non-axisymmetric aspheric lens by 2.5-axis CNC machine center have beenobtained. The results of grinding experiment show the new method can meet the need of grinding highaccuracy non-axisymmetric aspheric lens.
基金Supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) by the Ministry of Education, Science and Technology (No.NRF2016R1A2B4009626)
文摘AIM： To compare characteristics of preloaded and nonpreloaded intraocular lens（IOL） delivery systems during IOL delivery procedures. METHODS： Total 101 human eyes were included in this prospective observational case series. Delivery characteristics of 5 types of IOLs including i Sert250 NC60（NC60）, En Vista MX60（MX60）, Acry Sof IQ SN60 WF（SN60 WF）, TECNIS ZCB00（ZCB00）, and TECNIS PCB00（PCB00） were investigated. NC60 and PCB00 were injected via preloaded delivery systems and other IOLs were injected via nonpreloaded systems. In the human trial, time taken from IOL loading to completion of implantation was measured in all eyes undergoing conventional cataract surgery. Using 4 excised porcine eyes, dynamics of ophthalmic viscosurgical device（OVD） between an IOL injector and a porcine eye was analyzed using fluorescein sodiumstained OVD. RESULTS： The average time for IOL implantation was 22.0 s for NC60, 43.2 s for MX60, 32.3 s for SN60 WF, 41.4 s for ZCB00, and 14.6 s for PCB00 respectively. The number of cases with IOL manipulation with a second instrument was 6 for MX60, 2 for ZCB00, 0 for SN60 WF, NC60, and PCB00. Amount of OVD pushed into a porcine eye was smaller with a preloaded system than with non-preloaded systems. CONCLUSION： IOL delivery with preloaded systems is faster and more predictable. Moreover, a preloaded delivery system shows relatively less OVD pushed into a porcine eye than non-preloaded systems.
