AIM: To determine cytomegalovirus (CMV) frequency in neonatal intrahepatic cholestasis by serology, histological revision (searching for cytomegalic cells), immunohistochemistry, and polymerase chain reaction (PCR), a...AIM: To determine cytomegalovirus (CMV) frequency in neonatal intrahepatic cholestasis by serology, histological revision (searching for cytomegalic cells), immunohistochemistry, and polymerase chain reaction (PCR), and to verify the relationships among these methods.METHODS: The study comprised 101 non-consecutive infants submitted for hepatic biopsy between March 1982 and December 2005. Serological results were obtained from the patient's f iles and the other methods were performed on paraffin-embedded liver samples from hepatic biopsies. The following statistical measures were calculated: frequency, sensibility, specif ic positive predictive value, negative predictive value, and accuracy.RESULTS: The frequencies of positive results were as follows: serology, 7/64 (11%); histological revision, 0/84; immunohistochemistry, 1/44 (2%), and PCR, 6/77 (8%). Only one patient had positive immunohistochemical fi ndings and a positive PCR. The following statistical measures were calculated between PCR and serology: sensitivity, 33.3%; specificity, 88.89%; positive predictive value, 28.57%; negative predictive value, 90.91%; and accuracy, 82.35%. CONCLUSION: The frequency of positive CMV varied among the tests. Serology presented the highest positive frequency. When compared to PCR, the sensitivity and positive predictive value of serology were low.展开更多
新生儿个体化发育支持护理及评估（Neonatal Individualized Developmental Care and Assessment Program ，NIDCAP）自1986［1］年Dr ．Als 推广以来，已在国外很多医院实施于新生儿监护病房内的早产儿及高危新生儿。该项目以神经发育...新生儿个体化发育支持护理及评估（Neonatal Individualized Developmental Care and Assessment Program ，NIDCAP）自1986［1］年Dr ．Als 推广以来，已在国外很多医院实施于新生儿监护病房内的早产儿及高危新生儿。该项目以神经发育和心理发育为基础，强调对患儿的行为反应进行观察评估，采用个体化干预方案。我院2012年5月始，在新生儿重症监护病房设立了特殊照护（Special Care Nursery ， SCN）病房，对极低出生体重儿运用NIDACP模式进行护理，取得一定效果，现报告如下。展开更多
基金Supported by The State of So Paulo Research Foundation (Fapesp)and the Coordination for Higher Level Graduates Improvement (Capes)
文摘AIM: To determine cytomegalovirus (CMV) frequency in neonatal intrahepatic cholestasis by serology, histological revision (searching for cytomegalic cells), immunohistochemistry, and polymerase chain reaction (PCR), and to verify the relationships among these methods.METHODS: The study comprised 101 non-consecutive infants submitted for hepatic biopsy between March 1982 and December 2005. Serological results were obtained from the patient's f iles and the other methods were performed on paraffin-embedded liver samples from hepatic biopsies. The following statistical measures were calculated: frequency, sensibility, specif ic positive predictive value, negative predictive value, and accuracy.RESULTS: The frequencies of positive results were as follows: serology, 7/64 (11%); histological revision, 0/84; immunohistochemistry, 1/44 (2%), and PCR, 6/77 (8%). Only one patient had positive immunohistochemical fi ndings and a positive PCR. The following statistical measures were calculated between PCR and serology: sensitivity, 33.3%; specificity, 88.89%; positive predictive value, 28.57%; negative predictive value, 90.91%; and accuracy, 82.35%. CONCLUSION: The frequency of positive CMV varied among the tests. Serology presented the highest positive frequency. When compared to PCR, the sensitivity and positive predictive value of serology were low.
文摘新生儿个体化发育支持护理及评估（Neonatal Individualized Developmental Care and Assessment Program ，NIDCAP）自1986［1］年Dr ．Als 推广以来，已在国外很多医院实施于新生儿监护病房内的早产儿及高危新生儿。该项目以神经发育和心理发育为基础，强调对患儿的行为反应进行观察评估，采用个体化干预方案。我院2012年5月始，在新生儿重症监护病房设立了特殊照护（Special Care Nursery ， SCN）病房，对极低出生体重儿运用NIDACP模式进行护理，取得一定效果，现报告如下。
