为改善大豆蛋白的功能性质,利用中性蛋白酶和胰蛋白酶对大豆浓缩蛋白、分离蛋白进行限制性酶解处理,并考察相应产品的酶解修饰模式与溶解性、保水性变化的关系。以酶解修饰蛋白质产品的氮溶解指数、保水率、单分子层水含量为指标,测定...为改善大豆蛋白的功能性质,利用中性蛋白酶和胰蛋白酶对大豆浓缩蛋白、分离蛋白进行限制性酶解处理,并考察相应产品的酶解修饰模式与溶解性、保水性变化的关系。以酶解修饰蛋白质产品的氮溶解指数、保水率、单分子层水含量为指标,测定水解度(Drgree of Hydrolysis,DH)为1%、2%的8种大豆蛋白酶解修饰产品的溶解性、保水性与水吸附作用。结果表明,限制性酶解修饰处理后,酶解修饰产品的溶解性、保水性、水吸附作用的变化与酶解模式或DH有关;大豆浓缩蛋白经胰蛋白酶修饰至DH为1%,可以显著提高修饰产品的溶解性和保水性;大豆分离蛋白经胰蛋白酶或中性蛋白酶修饰后,可改善溶解性但破坏其保水性;大豆浓缩蛋白、分离蛋白的限制性酶解修饰处理,可以提高修饰产品对水的吸附作用。展开更多
Low molecular weight N-succinyl-chitosans with different degrees of substitution were synthesized by controlling reaction temperature, reaction time, and the molar ratio of the low molecular weight chitosan to succini...Low molecular weight N-succinyl-chitosans with different degrees of substitution were synthesized by controlling reaction temperature, reaction time, and the molar ratio of the low molecular weight chitosan to succinic anhydride. The structure of the low molecular weight N-succinyl-chitosan was characterized by infrared spectroscopy(IR), by which —COCH_ 2CH_ 2COOH was proved to be introduced to the —NH_ 2 of the low molecular weight chitosan. The moisture adsorption and moisture retention capacities of the low molecular weight chitosan derivatives with different degrees of substitution were investigated. The results indicate that the moisture adsorption and moisture retention capacities of the low molecular weight N-succinyl-chitosan increase with the increase of the degree of substitution. When the degree of substitution is greater than 38%, the derivatives have better moisture adsorption and moisture retention capacities than hyaluronic acid.展开更多
文摘为改善大豆蛋白的功能性质,利用中性蛋白酶和胰蛋白酶对大豆浓缩蛋白、分离蛋白进行限制性酶解处理,并考察相应产品的酶解修饰模式与溶解性、保水性变化的关系。以酶解修饰蛋白质产品的氮溶解指数、保水率、单分子层水含量为指标,测定水解度(Drgree of Hydrolysis,DH)为1%、2%的8种大豆蛋白酶解修饰产品的溶解性、保水性与水吸附作用。结果表明,限制性酶解修饰处理后,酶解修饰产品的溶解性、保水性、水吸附作用的变化与酶解模式或DH有关;大豆浓缩蛋白经胰蛋白酶修饰至DH为1%,可以显著提高修饰产品的溶解性和保水性;大豆分离蛋白经胰蛋白酶或中性蛋白酶修饰后,可改善溶解性但破坏其保水性;大豆浓缩蛋白、分离蛋白的限制性酶解修饰处理,可以提高修饰产品对水的吸附作用。
文摘Low molecular weight N-succinyl-chitosans with different degrees of substitution were synthesized by controlling reaction temperature, reaction time, and the molar ratio of the low molecular weight chitosan to succinic anhydride. The structure of the low molecular weight N-succinyl-chitosan was characterized by infrared spectroscopy(IR), by which —COCH_ 2CH_ 2COOH was proved to be introduced to the —NH_ 2 of the low molecular weight chitosan. The moisture adsorption and moisture retention capacities of the low molecular weight chitosan derivatives with different degrees of substitution were investigated. The results indicate that the moisture adsorption and moisture retention capacities of the low molecular weight N-succinyl-chitosan increase with the increase of the degree of substitution. When the degree of substitution is greater than 38%, the derivatives have better moisture adsorption and moisture retention capacities than hyaluronic acid.
