目的观察胃癌患者血浆配对盒蛋白5(paired box protein 5,PAX5)与环指蛋白180(ring finger protein 180,RNF180)基因甲基化情况,探讨其与临床病理特征及预后的关系。方法110例胃癌患者为胃癌组,同期体检健康者50例为对照组,采用甲基化...目的观察胃癌患者血浆配对盒蛋白5(paired box protein 5,PAX5)与环指蛋白180(ring finger protein 180,RNF180)基因甲基化情况,探讨其与临床病理特征及预后的关系。方法110例胃癌患者为胃癌组,同期体检健康者50例为对照组,采用甲基化特异性PCR法检测血浆PAX5与RNF180基因甲基化状态,分析其与胃癌患者临床病理特征的关系。随访3年,采用Kaplan-Meier生存曲线分析PAX5、RNF180甲基化与患者预后的关系。结果胃癌患者血浆PAX5、RNF180基因启动子区甲基化发生率(70.00%、62.73%)高于对照组(32.00%、30.00%)(P<0.05),胃癌组TNM分期Ⅰ~Ⅱ期患者血浆PAX5、RNF180基因甲基化发生率(65.17%、57.30%)低于TNM分期Ⅲ~Ⅳ期患者(90.48%、85.71%)(P<0.05),胃癌组男性与女性、年龄<55岁与≥55岁、肿瘤直径<2.5 cm与≥2.5 cm、细胞低-中分化与高分化及有无淋巴结转移患者血浆PAX5、RNF180基因甲基化发生率比较差异均无统计学意义(P>0.05)。发生PAX5基因甲基化的胃癌患者3年生存率(68.83%)低于未发生甲基化患者(84.85%)(P<0.05),发生RNF180基因甲基化的胃癌患者3年总体生存率(73.91%)与未发生甲基化胃癌患者(73.17%)比较差异无统计学意义(P>0.05)。结论胃癌患者血浆PAX5与RNF180基因甲基化发生率增高,与肿瘤TNM分期有关,发生PAX5基因甲基化的患者3年总生存率低。展开更多
Mercury methylation rates in fresh and pre dried river sediments were determined in sediment water systems spiked with 203 HgCl 2 at a level of 0.75—0.95 ppm as Hg. At the end of the incubation period (21...Mercury methylation rates in fresh and pre dried river sediments were determined in sediment water systems spiked with 203 HgCl 2 at a level of 0.75—0.95 ppm as Hg. At the end of the incubation period (21—38 days), methylmercury production in the fresh sediments was in the order of 3.0% to 13.75%, whereas in the pre dried sediments the methylation was much lower, at 0.6% to 3.9%. The highest methylation levels were generally recorded in the uppermost, 1 cm layer of the sediment column. Total mercury concentration in water in equilibrium with the 203 Hg spiked fresh sediments was in the range of 44.7 to 634 ng/L, whereas in equilibrium with the pre dried sediments the concentration was in the range of 19.5 to 34.5 ng/L. The proportion of methylmercury to total mercury in the water varied from 9.4% to 66.6% over the fresh sediments, and from 7.1% to 10.8% over the pre dried sediments. In the system consisting of water, sediment and sediment inverterbrates the concentrations of methylmercury were 8.61 to 15.69 ng/g in the sediment, 0.011 ng/ml in the water, and 9.22 to 40.69 ng/g in the inverterbrates. Methylmercury bioaccumualtion factors (BAFs) were in the order of 274 to 8087 relative to the water, and 0.25 to 7.31 relative to the sediment. These results provide further evidence for the tendency of methylmercury to accumulate in high concentrations in aquatic organisms, even at very low total mercury concentration in water.展开更多
为建立一种评估重亚硫酸盐处理DNA样本后胞嘧啶转化效率的有效方法,以两组不同的Taq Man q PCR检测梯度稀释的重亚硫酸盐处理和未处理的DNA标准品,建立转化与未转化的DNA Ct值以及对应的DNA拷贝数的标准曲线。使用相同的探针定量检测重...为建立一种评估重亚硫酸盐处理DNA样本后胞嘧啶转化效率的有效方法,以两组不同的Taq Man q PCR检测梯度稀释的重亚硫酸盐处理和未处理的DNA标准品,建立转化与未转化的DNA Ct值以及对应的DNA拷贝数的标准曲线。使用相同的探针定量检测重亚硫酸盐处理后的DNA样本评估转化效率。结果显示该方法应用两组探针,根据相应的标准曲线,精确评估样本经重亚硫酸盐处理的转化效率。使用已知转化和未转化拷贝数的混合DNA作为模板,证实了该方法的可靠性。同时也对不同重亚硫酸盐试剂盒处理DNA的转化效率进行了评估,结果显示,该方法能够有效地评估DNA样品重亚硫酸盐的转化效率,为DNA甲基化准确分析提供了可靠快捷的方法。展开更多
文摘目的观察胃癌患者血浆配对盒蛋白5(paired box protein 5,PAX5)与环指蛋白180(ring finger protein 180,RNF180)基因甲基化情况,探讨其与临床病理特征及预后的关系。方法110例胃癌患者为胃癌组,同期体检健康者50例为对照组,采用甲基化特异性PCR法检测血浆PAX5与RNF180基因甲基化状态,分析其与胃癌患者临床病理特征的关系。随访3年,采用Kaplan-Meier生存曲线分析PAX5、RNF180甲基化与患者预后的关系。结果胃癌患者血浆PAX5、RNF180基因启动子区甲基化发生率(70.00%、62.73%)高于对照组(32.00%、30.00%)(P<0.05),胃癌组TNM分期Ⅰ~Ⅱ期患者血浆PAX5、RNF180基因甲基化发生率(65.17%、57.30%)低于TNM分期Ⅲ~Ⅳ期患者(90.48%、85.71%)(P<0.05),胃癌组男性与女性、年龄<55岁与≥55岁、肿瘤直径<2.5 cm与≥2.5 cm、细胞低-中分化与高分化及有无淋巴结转移患者血浆PAX5、RNF180基因甲基化发生率比较差异均无统计学意义(P>0.05)。发生PAX5基因甲基化的胃癌患者3年生存率(68.83%)低于未发生甲基化患者(84.85%)(P<0.05),发生RNF180基因甲基化的胃癌患者3年总体生存率(73.91%)与未发生甲基化胃癌患者(73.17%)比较差异无统计学意义(P>0.05)。结论胃癌患者血浆PAX5与RNF180基因甲基化发生率增高,与肿瘤TNM分期有关,发生PAX5基因甲基化的患者3年总生存率低。
文摘Mercury methylation rates in fresh and pre dried river sediments were determined in sediment water systems spiked with 203 HgCl 2 at a level of 0.75—0.95 ppm as Hg. At the end of the incubation period (21—38 days), methylmercury production in the fresh sediments was in the order of 3.0% to 13.75%, whereas in the pre dried sediments the methylation was much lower, at 0.6% to 3.9%. The highest methylation levels were generally recorded in the uppermost, 1 cm layer of the sediment column. Total mercury concentration in water in equilibrium with the 203 Hg spiked fresh sediments was in the range of 44.7 to 634 ng/L, whereas in equilibrium with the pre dried sediments the concentration was in the range of 19.5 to 34.5 ng/L. The proportion of methylmercury to total mercury in the water varied from 9.4% to 66.6% over the fresh sediments, and from 7.1% to 10.8% over the pre dried sediments. In the system consisting of water, sediment and sediment inverterbrates the concentrations of methylmercury were 8.61 to 15.69 ng/g in the sediment, 0.011 ng/ml in the water, and 9.22 to 40.69 ng/g in the inverterbrates. Methylmercury bioaccumualtion factors (BAFs) were in the order of 274 to 8087 relative to the water, and 0.25 to 7.31 relative to the sediment. These results provide further evidence for the tendency of methylmercury to accumulate in high concentrations in aquatic organisms, even at very low total mercury concentration in water.
文摘为建立一种评估重亚硫酸盐处理DNA样本后胞嘧啶转化效率的有效方法,以两组不同的Taq Man q PCR检测梯度稀释的重亚硫酸盐处理和未处理的DNA标准品,建立转化与未转化的DNA Ct值以及对应的DNA拷贝数的标准曲线。使用相同的探针定量检测重亚硫酸盐处理后的DNA样本评估转化效率。结果显示该方法应用两组探针,根据相应的标准曲线,精确评估样本经重亚硫酸盐处理的转化效率。使用已知转化和未转化拷贝数的混合DNA作为模板,证实了该方法的可靠性。同时也对不同重亚硫酸盐试剂盒处理DNA的转化效率进行了评估,结果显示,该方法能够有效地评估DNA样品重亚硫酸盐的转化效率,为DNA甲基化准确分析提供了可靠快捷的方法。
