该文研究了3种药用植物来源的蜂蜜(茴香蜜、枸杞蜜和玄参蜜)的抗氧化能力和抗菌活性,并与麦卢卡蜜进行对比分析。用DPPH自由基、ABTS阳离子自由基清除能力和铁离子还原/抗氧化能力(ferric ion reducing/antioxidant power,FRAP)评价蜂...该文研究了3种药用植物来源的蜂蜜(茴香蜜、枸杞蜜和玄参蜜)的抗氧化能力和抗菌活性,并与麦卢卡蜜进行对比分析。用DPPH自由基、ABTS阳离子自由基清除能力和铁离子还原/抗氧化能力(ferric ion reducing/antioxidant power,FRAP)评价蜂蜜的抗氧化能力。琼脂扩散法(抑菌圈)和微量肉汤稀释法用于表征蜂蜜对革兰氏阳性菌(金黄色葡萄球菌和表皮葡萄球菌)、革兰氏阴性菌(大肠杆菌、鲍曼不动杆菌和铜绿假单胞菌)和真菌(白色念珠菌)的抗微生物能力。结果表明,麦卢卡蜜的总酚含量和抗氧化能力高于其他3种蜂蜜。蜂蜜的抗氧化能力和电导率、色值、总酚含量之间具有相关性(r≥0.727,P<0.01)。茴香蜜对金黄色葡萄球菌和表皮葡萄球菌的抗菌能力最强。茴香蜜和枸杞蜜对鲍曼不动杆菌和铜绿假单胞菌的抗菌能力高于麦卢卡蜜。麦卢卡蜜对大肠杆菌的抗菌活性高于采集的茴香蜜、枸杞蜜和玄参蜜。3种药用植物来源蜂蜜的抗菌活性与pH、淀粉酶活性和过氧化氢含量呈线性相关。展开更多
Cancer occurs when the body’s cells grow beyond the usual control. Normal body cells multiply in a controlled manner and undergo apoptosis when the body no longer needs them. Different types of cancers exist, and the...Cancer occurs when the body’s cells grow beyond the usual control. Normal body cells multiply in a controlled manner and undergo apoptosis when the body no longer needs them. Different types of cancers exist, and the common ones include breast, cervical, prostate, lung, colon, and skin cancers. Several factors have been associated with cancers, and these factors include poor dieting, exposure to harmful chemicals and radiation, weak immune system, and genetics. Cancer presents an enormous health threat in the modern world and thus the need to identify an effective treatment. The conventional treatments used in the management of cancer include chemotherapy and physiotherapy. These forms of cancer treatments usually have enormous side effects on the subjects. In this respect, an alternative form of treatment would be effective in managing cancer patients. A substantial number of natural products have been observed to be effective in the management of cancer. These natural products include plants and other natural substances such as honey. This study focuses on the efficiency of natural products in the treatment of cancer. Also, the anticancer effects of Peganum harmala, Manuka honey, and Saudi honey will be analyzed. Bee honey and Peganum harmala have been traditionally used in the treatment of cancer. The extracts from Peganum harmala plant have also been shown to exhibit divers’ antitumor effects similar to the mode of action of a vast number of anticancer agents. These established hypotheses thus give the rationale for this study. In this experiment, extracts were obtained from Peganum harmala leaves and exposed to cervical, lung, and prostate cancer cells. Similarly, solutions of Manuka honey and Saudi honey were exposed to the cervical, lung, and prostate cancer cells. The experiment duration was 24 hours, which obtained results were recorded and analyzed. Peganum harmala extracts inhibit cancer cell growth at different and achievable concentrations. Manuka honey highly inhibits the growth of HeLa cancer c展开更多
目的建立高效液相色谱-荧光检测法(high performance liquidchromatography with fluorescence detector,HPLC-FLD)测定麦卢卡蜂蜜中特征标识物3,5-二甲氧基苯甲酸甲酯-4-双葡萄糖糖苷(leptosperin)的方法。方法以Platisil ODS色谱柱(15...目的建立高效液相色谱-荧光检测法(high performance liquidchromatography with fluorescence detector,HPLC-FLD)测定麦卢卡蜂蜜中特征标识物3,5-二甲氧基苯甲酸甲酯-4-双葡萄糖糖苷(leptosperin)的方法。方法以Platisil ODS色谱柱(150 mm×4.6 mm, 5μm)为分析柱,甲醇-水为流动相进行等度洗脱,使用荧光检测器,外标法定量。结果在0.5～100 mg/L范围内目标化合物的线性关系良好,相关系数为0.9999,方法的检出限(limit of detection LOD,S/N≥3)和定量限(limit of quantitation,LOQ,S/N≥10)分别为2mg/kg和8 mg/kg。在10、20、50 mg/kg浓度水平下平均回收率为82.2%～108.9%,相对标准偏差为1.3%～7.5%(n=6)。结论该方法前处理方法简单快速,测定结果准确可靠,可为麦卢卡蜂蜜真伪鉴别提供参考。展开更多
目的运用新西兰政府初级产业部(Ministry of Primary Industry, MPI)提出的多重qPCR方法鉴定麦卢卡蜂蜜。方法参照MPI提出的多重qPCR方法,建立麦卢卡标准曲线,提取15个未知来源的蜂蜜样品样品中的花粉DNA,进行样品多重qPCR检测分析。结...目的运用新西兰政府初级产业部(Ministry of Primary Industry, MPI)提出的多重qPCR方法鉴定麦卢卡蜂蜜。方法参照MPI提出的多重qPCR方法,建立麦卢卡标准曲线,提取15个未知来源的蜂蜜样品样品中的花粉DNA,进行样品多重qPCR检测分析。结果建立麦卢卡标准曲线获得Manuka通道的阈值范围,可以用于今后蜂蜜样品的麦卢卡特异性分析。M2-4蜂蜜样品中的花粉DNA不来自麦卢卡树Leptospermum scoparium。结论本研究对MPI提出的鉴定麦卢卡蜂蜜方法起到很好的推动及指导作用。展开更多
文摘该文研究了3种药用植物来源的蜂蜜(茴香蜜、枸杞蜜和玄参蜜)的抗氧化能力和抗菌活性,并与麦卢卡蜜进行对比分析。用DPPH自由基、ABTS阳离子自由基清除能力和铁离子还原/抗氧化能力(ferric ion reducing/antioxidant power,FRAP)评价蜂蜜的抗氧化能力。琼脂扩散法(抑菌圈)和微量肉汤稀释法用于表征蜂蜜对革兰氏阳性菌(金黄色葡萄球菌和表皮葡萄球菌)、革兰氏阴性菌(大肠杆菌、鲍曼不动杆菌和铜绿假单胞菌)和真菌(白色念珠菌)的抗微生物能力。结果表明,麦卢卡蜜的总酚含量和抗氧化能力高于其他3种蜂蜜。蜂蜜的抗氧化能力和电导率、色值、总酚含量之间具有相关性(r≥0.727,P<0.01)。茴香蜜对金黄色葡萄球菌和表皮葡萄球菌的抗菌能力最强。茴香蜜和枸杞蜜对鲍曼不动杆菌和铜绿假单胞菌的抗菌能力高于麦卢卡蜜。麦卢卡蜜对大肠杆菌的抗菌活性高于采集的茴香蜜、枸杞蜜和玄参蜜。3种药用植物来源蜂蜜的抗菌活性与pH、淀粉酶活性和过氧化氢含量呈线性相关。
文摘Cancer occurs when the body’s cells grow beyond the usual control. Normal body cells multiply in a controlled manner and undergo apoptosis when the body no longer needs them. Different types of cancers exist, and the common ones include breast, cervical, prostate, lung, colon, and skin cancers. Several factors have been associated with cancers, and these factors include poor dieting, exposure to harmful chemicals and radiation, weak immune system, and genetics. Cancer presents an enormous health threat in the modern world and thus the need to identify an effective treatment. The conventional treatments used in the management of cancer include chemotherapy and physiotherapy. These forms of cancer treatments usually have enormous side effects on the subjects. In this respect, an alternative form of treatment would be effective in managing cancer patients. A substantial number of natural products have been observed to be effective in the management of cancer. These natural products include plants and other natural substances such as honey. This study focuses on the efficiency of natural products in the treatment of cancer. Also, the anticancer effects of Peganum harmala, Manuka honey, and Saudi honey will be analyzed. Bee honey and Peganum harmala have been traditionally used in the treatment of cancer. The extracts from Peganum harmala plant have also been shown to exhibit divers’ antitumor effects similar to the mode of action of a vast number of anticancer agents. These established hypotheses thus give the rationale for this study. In this experiment, extracts were obtained from Peganum harmala leaves and exposed to cervical, lung, and prostate cancer cells. Similarly, solutions of Manuka honey and Saudi honey were exposed to the cervical, lung, and prostate cancer cells. The experiment duration was 24 hours, which obtained results were recorded and analyzed. Peganum harmala extracts inhibit cancer cell growth at different and achievable concentrations. Manuka honey highly inhibits the growth of HeLa cancer c
文摘目的建立高效液相色谱-荧光检测法(high performance liquidchromatography with fluorescence detector,HPLC-FLD)测定麦卢卡蜂蜜中特征标识物3,5-二甲氧基苯甲酸甲酯-4-双葡萄糖糖苷(leptosperin)的方法。方法以Platisil ODS色谱柱(150 mm×4.6 mm, 5μm)为分析柱,甲醇-水为流动相进行等度洗脱,使用荧光检测器,外标法定量。结果在0.5～100 mg/L范围内目标化合物的线性关系良好,相关系数为0.9999,方法的检出限(limit of detection LOD,S/N≥3)和定量限(limit of quantitation,LOQ,S/N≥10)分别为2mg/kg和8 mg/kg。在10、20、50 mg/kg浓度水平下平均回收率为82.2%～108.9%,相对标准偏差为1.3%～7.5%(n=6)。结论该方法前处理方法简单快速,测定结果准确可靠,可为麦卢卡蜂蜜真伪鉴别提供参考。
