AIM: To determine whether the mast cell (MCs) and tumor-associated macrophage (TAMs) counts have any correlation with clinical outcome in colorectal cancer, and to investigate whether MCs undergo phenotypic changes in...AIM: To determine whether the mast cell (MCs) and tumor-associated macrophage (TAMs) counts have any correlation with clinical outcome in colorectal cancer, and to investigate whether MCs undergo phenotypic changes in colorectal cancer.METHODS: The MC and TAM counts were determined immunohistochemically in 60 patients with colorectal cancer and the depth of invasion, lymph node metastasis rate, distant metastasis rates, and survival rates were compared between patients with low (less than the mean number of positive cells) and high (more than the mean number of positive cells) cell counts.RESULTS: Both patients with a low MC count and patients with a low TAM count had significantly deeper depth of invasion than those with a high MC count and those with a high TAM count (P＜0.01 and P＜0.01 respectively).Patients with a high MC count and patients with a high TAM count were significantly higher showing significantly lower rates of lymph node metastasis, distant metastasis than those with a low MC count and those with a low TAM count. There were significant positive correlation between MC counts and TAM counts (r = 0.852, P＜0.01).In both cancerous tissue and normal colorectal tissue,the predominant MC phenotype was MCTC. The 5-year survival rate estimated was significantly lower in both patients with a low MC count and patients with a low TAM count than in those with a high MC count and those with a high TAM count (P＜0.05 and P＜0.01 respectively).CONCLUSION: There appears to be a direct relationship between the number of MCs and clinical outcome in patients with colorectal cancer, even though MCs exhibited no significant phenotypic changes. TAM count is of value to predict the clinical outcome or prognosis. It is more beneficial for estimating biological character of colorectal carcinoma to combine MC and TAM counts.展开更多
通过人参水提液(water extract of Ginseng,WEG)、人参多糖(Ginseng polysaccharides,GPS)、人参皂苷(Ginseng total saponins,GTS)对肿瘤相关巨噬细胞(tumor-associated macrophages,TAMs)与肿瘤细胞共培养体系中肺癌A549细胞增殖、迁...通过人参水提液(water extract of Ginseng,WEG)、人参多糖(Ginseng polysaccharides,GPS)、人参皂苷(Ginseng total saponins,GTS)对肿瘤相关巨噬细胞(tumor-associated macrophages,TAMs)与肿瘤细胞共培养体系中肺癌A549细胞增殖、迁移和细胞骨架的影响研究,探讨人参抗肿瘤的作用机制。建立THP-1诱导的TAMs体外模型,采用上清液共培养体系,以肺癌A549细胞为研究对象,并采用MTT法观察不同浓度的WEG、GPS、GTS作用于共培养体系中对肺癌A549细胞增殖的影响及量效关系;通过实时细胞分析技术(RTCA)检测WEG、GPS、GTS对共培养体系中肺癌A549细胞迁移能力的影响,免疫荧光高内涵细胞分析系统(HCS)检测细胞骨架蛋白F-actin的表达。结果显示:WEG能明显抑制共培养体系中A549细胞的增殖、迁移能力和减少骨架面积及微丝数量(P<0.01);GPS能明显抑制共培养体系中A549细胞迁移能力、骨架面积和微丝数量(P<0.01),但对A549细胞的增殖作用无明显影响;GTS能明显抑制共培养体系中A549细胞的增殖(P<0.01),而对A549细胞的迁移能力、骨架面积和微丝无影响。通过对WEG、GPS、GTS的研究对比,发现人参及两种主要成分对TAMs共培养体系中肺癌A549细胞都有影响,但作用存在差异,提示人参抗肿瘤作用与其多成分对肿瘤免疫微环境调控相关,且不同成分间可能存在协同关系。展开更多
基金Supported by "Tenth Five-Year" Key Project of Scientific and Technological Bureau of Hubei Province, No. 2002AA301C59
文摘AIM: To determine whether the mast cell (MCs) and tumor-associated macrophage (TAMs) counts have any correlation with clinical outcome in colorectal cancer, and to investigate whether MCs undergo phenotypic changes in colorectal cancer.METHODS: The MC and TAM counts were determined immunohistochemically in 60 patients with colorectal cancer and the depth of invasion, lymph node metastasis rate, distant metastasis rates, and survival rates were compared between patients with low (less than the mean number of positive cells) and high (more than the mean number of positive cells) cell counts.RESULTS: Both patients with a low MC count and patients with a low TAM count had significantly deeper depth of invasion than those with a high MC count and those with a high TAM count (P＜0.01 and P＜0.01 respectively).Patients with a high MC count and patients with a high TAM count were significantly higher showing significantly lower rates of lymph node metastasis, distant metastasis than those with a low MC count and those with a low TAM count. There were significant positive correlation between MC counts and TAM counts (r = 0.852, P＜0.01).In both cancerous tissue and normal colorectal tissue,the predominant MC phenotype was MCTC. The 5-year survival rate estimated was significantly lower in both patients with a low MC count and patients with a low TAM count than in those with a high MC count and those with a high TAM count (P＜0.05 and P＜0.01 respectively).CONCLUSION: There appears to be a direct relationship between the number of MCs and clinical outcome in patients with colorectal cancer, even though MCs exhibited no significant phenotypic changes. TAM count is of value to predict the clinical outcome or prognosis. It is more beneficial for estimating biological character of colorectal carcinoma to combine MC and TAM counts.
文摘通过人参水提液(water extract of Ginseng,WEG)、人参多糖(Ginseng polysaccharides,GPS)、人参皂苷(Ginseng total saponins,GTS)对肿瘤相关巨噬细胞(tumor-associated macrophages,TAMs)与肿瘤细胞共培养体系中肺癌A549细胞增殖、迁移和细胞骨架的影响研究,探讨人参抗肿瘤的作用机制。建立THP-1诱导的TAMs体外模型,采用上清液共培养体系,以肺癌A549细胞为研究对象,并采用MTT法观察不同浓度的WEG、GPS、GTS作用于共培养体系中对肺癌A549细胞增殖的影响及量效关系;通过实时细胞分析技术(RTCA)检测WEG、GPS、GTS对共培养体系中肺癌A549细胞迁移能力的影响,免疫荧光高内涵细胞分析系统(HCS)检测细胞骨架蛋白F-actin的表达。结果显示:WEG能明显抑制共培养体系中A549细胞的增殖、迁移能力和减少骨架面积及微丝数量(P<0.01);GPS能明显抑制共培养体系中A549细胞迁移能力、骨架面积和微丝数量(P<0.01),但对A549细胞的增殖作用无明显影响;GTS能明显抑制共培养体系中A549细胞的增殖(P<0.01),而对A549细胞的迁移能力、骨架面积和微丝无影响。通过对WEG、GPS、GTS的研究对比,发现人参及两种主要成分对TAMs共培养体系中肺癌A549细胞都有影响,但作用存在差异,提示人参抗肿瘤作用与其多成分对肿瘤免疫微环境调控相关,且不同成分间可能存在协同关系。
