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Comparative Analysis of Fingerprint Spectra of Fruits and Leaves of Prunus mume Collected at Various Altitudes in Different Months 被引量:1
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作者 Qian JIN Xue ZHAI +3 位作者 Fang XIAO Zhe LIU Yongxia KE Yuan LIU 《Medicinal Plant》 2017年第4期23-26,31,共5页
[Objectives] To study characteristic fingerprint spectra of fruits( its stones were removed) and leaves of Prunus mume collected at various altitudes in different regions of Jinchuan County,the Aba Tibetan and Qiang A... [Objectives] To study characteristic fingerprint spectra of fruits( its stones were removed) and leaves of Prunus mume collected at various altitudes in different regions of Jinchuan County,the Aba Tibetan and Qiang Autonomous Prefecture,Sichuan Province,China in different months by using Ultra Performance Liquid Chromatography( UPLC).[Methods]The evaluation system of similarity of chromatographic fingerprint spectra of traditional Chinese medicine and SPSS19. 0 were used to assess their similarity and to establish the reasonable reliable common model of chromatographic fingerprints,and the quality of the medicinal materials was evaluated.[Results] Seen from the fingerprint spectra of fruits( its stones were removed) and leaves of P. mume collected at different altitudes in various months,there were 15 common peaks in the fingerprint spectra of the fruits( its stones were removed),and the similarity of the 28 samples was higher than 0. 900,showing that there were small differences between the samples of the fruits( its stones were removed) in chemical components; there were 10 common peaks in the fingerprint spectra of the leaves,and the similarity of the 28 samples was also higher than 0. 900,indicating that there were also small differences between the samples of the leaves in chemical components.[Conclusions] The method of using fingerprint spectra to control the quality of fruits and leaves of P. mume was simple and stable and had strong specificity and good repeatability. 展开更多
关键词 FRUITS of prunus mume leaves of prunus mume UPLC Characteristic FINGERPRINT SPECTRA Quality evaluation
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不同激素对‘美人’梅叶片离体培养的影响及其细胞学观察 被引量:2
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作者 张秦英 陈俊愉 申作连 《北京林业大学学报》 CAS CSCD 北大核心 2004年第S1期42-44,169,共4页
该试验以‘美人’梅离体培养的无菌苗叶片为材料 ,研究了不同种类激素配比对叶片脱分化及再分化的影响 ,并对不同条件下产生的愈伤组织进行了细胞学观察。结果表明 :BA对‘美人’梅叶片的愈伤组织诱导起到抑制作用 ;NAA可使叶片直接生根... 该试验以‘美人’梅离体培养的无菌苗叶片为材料 ,研究了不同种类激素配比对叶片脱分化及再分化的影响 ,并对不同条件下产生的愈伤组织进行了细胞学观察。结果表明 :BA对‘美人’梅叶片的愈伤组织诱导起到抑制作用 ;NAA可使叶片直接生根 ;2 ,4 D的加入可以诱发叶片形成球形愈伤。但只有配合适当浓度的BA ,才能形成胚状体。诱导胚状体合适的培养基为 :WPM +BA 1mg L +NAA 0 1mg L +2 ,4 D 1mg L +L 谷氨酰胺 10 0mg L +水解乳蛋白 10 0 0mg L。 展开更多
关键词 梅叶片 激素 不定根 体细胞胚发生 细胞观察
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