摘要
[Objectives] To study characteristic fingerprint spectra of fruits( its stones were removed) and leaves of Prunus mume collected at various altitudes in different regions of Jinchuan County,the Aba Tibetan and Qiang Autonomous Prefecture,Sichuan Province,China in different months by using Ultra Performance Liquid Chromatography( UPLC).[Methods]The evaluation system of similarity of chromatographic fingerprint spectra of traditional Chinese medicine and SPSS19. 0 were used to assess their similarity and to establish the reasonable reliable common model of chromatographic fingerprints,and the quality of the medicinal materials was evaluated.[Results] Seen from the fingerprint spectra of fruits( its stones were removed) and leaves of P. mume collected at different altitudes in various months,there were 15 common peaks in the fingerprint spectra of the fruits( its stones were removed),and the similarity of the 28 samples was higher than 0. 900,showing that there were small differences between the samples of the fruits( its stones were removed) in chemical components; there were 10 common peaks in the fingerprint spectra of the leaves,and the similarity of the 28 samples was also higher than 0. 900,indicating that there were also small differences between the samples of the leaves in chemical components.[Conclusions] The method of using fingerprint spectra to control the quality of fruits and leaves of P. mume was simple and stable and had strong specificity and good repeatability.
[Objectives] To study characteristic fingerprint spectra of fruits( its stones were removed) and leaves of Prunus mume collected at various altitudes in different regions of Jinchuan County,the Aba Tibetan and Qiang Autonomous Prefecture,Sichuan Province,China in different months by using Ultra Performance Liquid Chromatography( UPLC).[Methods]The evaluation system of similarity of chromatographic fingerprint spectra of traditional Chinese medicine and SPSS19. 0 were used to assess their similarity and to establish the reasonable reliable common model of chromatographic fingerprints,and the quality of the medicinal materials was evaluated.[Results] Seen from the fingerprint spectra of fruits( its stones were removed) and leaves of P. mume collected at different altitudes in various months,there were 15 common peaks in the fingerprint spectra of the fruits( its stones were removed),and the similarity of the 28 samples was higher than 0. 900,showing that there were small differences between the samples of the fruits( its stones were removed) in chemical components; there were 10 common peaks in the fingerprint spectra of the leaves,and the similarity of the 28 samples was also higher than 0. 900,indicating that there were also small differences between the samples of the leaves in chemical components.[Conclusions] The method of using fingerprint spectra to control the quality of fruits and leaves of P. mume was simple and stable and had strong specificity and good repeatability.
基金
Supported by 2014 Sichuan Science and Technology Support Plan Program(2014SZ0131)
