Mikania micrantha Kunth is an invasive alien weed and known as a plant killer around the world.Accurately and rapidly identifying M.micrantha in the wild is important for monitoring its growth status,as this helps man...Mikania micrantha Kunth is an invasive alien weed and known as a plant killer around the world.Accurately and rapidly identifying M.micrantha in the wild is important for monitoring its growth status,as this helps management officials to take the necessary steps to devise a comprehensive strategy to control the invasive weed in the identified area.However,this approach still mainly depends on satellite remote sensing and manual inspection.The cost is high and the accuracy rate and efficiency are low.We acquired color images of the monitoring area in the wild environment using an Unmanned Aerial Vehicle(UAV)and proposed a novel network-MmNet-based on a deep Convolutional Neural Network(CNN)to identify M.micrantha in the images.The network consists of AlexNet Local Response Normalization(LRN),along with the GoogLeNet and continuous convolution of VGG inception models.After training and testing,the identification of 400 testing samples by MmNet is very good,with accuracy of 94.50%and time cost of 10.369 s.Moreover,in quantitative comparative analysis,the proposed MmNet not only has high accuracy and efficiency but also simple construction and outstanding repeatability.Compared with recently popular CNNs,MmNet is more suitable for the identification of M.micrantha in the wild.However,to meet the challenge of wild environments,more M.micrantha images need to be acquired for MmNet training.In addition,the classification labels need to be sorted in more detail.Altogether,this research provides some theoretical and scientific basis for the development of intelligent monitoring and early warning systems for M.micrantha and other invasive species.展开更多
Objective: To optimize the ionic liquid based microwave-assisted extraction (IL-MAE) of polyphenolic content from Peperomia pellucida (L) Kunth. Methods: The IL-MAE factors as experimental design parameters, including...Objective: To optimize the ionic liquid based microwave-assisted extraction (IL-MAE) of polyphenolic content from Peperomia pellucida (L) Kunth. Methods: The IL-MAE factors as experimental design parameters, including microwave power, extraction time, ionic liquid concentration, and liquid–solid ratio had been involved. Response surface methodology and Box–Behnken design were used to obtain predictive model (multivariate quadratic regression equation) and optimization of the extraction process. The response surface was analyzed by using the yields of total polyphenolic content as response value. Results: Based on the obtained results the optimum extraction condition, including microwave power of 30% Watts, extraction time of 18.5 min, the ionic liquid concen-tration of 0.79 mol/L, and the liquid–solid ratio of 10.72 mL/g 1-Buthyl-3-methylimidazolium tetrafluoroborate ([bmim]BF4) as a solvent was selected. The regression model was obtained to predicts the yields from Peperomia pellucida:Y = 30.250 – 1.356X1 + 2.655X2 + 2.252X3 – 0.565X4 + 0.990 X1X3 – 8.172 X1X4 – 3.439 X3X4 – 4.178 X12 – 3.210 X32 – 6.786 X42 – 7.290 X12X3 + 5.575 X1X32 – 4.843 X32X4 with R2 = 0.82519. Scale-up confirmation test was obtained the maximum yields of total polyphenolics content with the amount of 31.1725μg GAE/g. Conclusions: The IL-MAE method produced a higher extraction polyphenolic and performed rapidly, easily and efficiently.展开更多
Two new isospirostanol sapogenins named （25R）-5/%spirostane-（1αα 3a）-diol （1） and （25R）-5/%spirostane-（1α, 2α, 3α, 4α）- tetrol （2） were isolated from the whole herb ofReineckia carnea （Andr.） Kunth...Two new isospirostanol sapogenins named （25R）-5/%spirostane-（1αα 3a）-diol （1） and （25R）-5/%spirostane-（1α, 2α, 3α, 4α）- tetrol （2） were isolated from the whole herb ofReineckia carnea （Andr.） Kunth. The structures of the sapogenins were elucidated by chemical and spectral methods. methods展开更多
Objective:To isolate,identify,and evaluate a new angiotensin-converting enzyme inhibitor from Peperomia pellucida(L.)Kunth herbs.Methods:A dried sample of Peperomia pellucida herb was successively macerated with n-hex...Objective:To isolate,identify,and evaluate a new angiotensin-converting enzyme inhibitor from Peperomia pellucida(L.)Kunth herbs.Methods:A dried sample of Peperomia pellucida herb was successively macerated with n-hexane and ethyl acetate.The ethyl acetate extract solution was evaporated to obtain the crude extract.Vacuum liquid column chromatography and thin layer chromatography were performed to obtain two pure compounds.Then,both compounds were elucidated and identified using the spectroscopic method.Angiotensin-converting enzyme inhibitory activity studies of both compounds were determined using angiotensin-converting enzyme kit WST-1 with spectrophotometer microplate reader 96-well at 450 nm wavelength.Results:Two bioactive compounds were successfully isolated from Peperomia pellucida herb,including a new compound of 2,3,5-trimethoxy-9-(12,14,15-trimethoxybenzyl)-1 H-indene and pellucidin A.Both compounds demonstrated angiotensin-converting enzyme inhibitory activity,with IC50 values of 72 μM(27.95 μg/mL)and 1 1μM(4.4 μg/mL),respectively.Conclusions:In the present study,two active angiotensin-converting enzyme inhibitors were successfully isolated and purified from Peperomia pellucida which is used as an antihypertensive in traditional medicine,and support its use as an angiotensin-converting enzyme-inhibiting drug.展开更多
[Objective] The control methods of the bulb rot of Iphigenia indica Kunth.caused by Fusarium orthoceras App.et Wr.var.longius （Sherb.） were explored.[Method] The effect of eight different kinds of metal ions on the ...[Objective] The control methods of the bulb rot of Iphigenia indica Kunth.caused by Fusarium orthoceras App.et Wr.var.longius （Sherb.） were explored.[Method] The effect of eight different kinds of metal ions on the pathogen was studied;the contents of seven different kinds of metal ions in rhizosphere soil and bulb were also determined.[Result] The results showed that when the concentration of Cu2＋ or Mg2＋ was greater than 1.0mg/L or the concentration of Zn2＋ or Ca2＋ was lower than 0.5mg/L,the growth of pathogen could be inhibited.The results also showed that the bulb could enrich K highly,enrich Zn and Mg,limit the absorption of Ca and Fe,and inhibit the absorption of Cu and Mn during the growth process of bulb in I.indica Kunth.[Conclusion] Micronutrient fertilizer containing Mg could be used to prevent and cure the bulb rot of I.indica Kunth.展开更多
Piper auritum Kunth,a member of the Piperacea family,is a small shrub native to the Central American tropics.This plant was traditionally used in Latin American countries for the treatment of various medical condition...Piper auritum Kunth,a member of the Piperacea family,is a small shrub native to the Central American tropics.This plant was traditionally used in Latin American countries for the treatment of various medical conditions.Increase in the recent studies of P.auritum’s chemical components leads to the isolation of monoterpenoids,sesquiterpenoids,phenylpropanoids and several other compounds.Previous research has revealed that these secondary metabolites have diverse pharmacological activities including antidiabetic,antioxidant,anti-inflammatory,anti-pathogeny microorganism and repellent activity.This review summarizes relevant findings on P.auritum,and hopes to contribute to the exploration of its potentials for further development as an alternative medicine.展开更多
基金supported by the National Natural Science Foundation of China(3180111238)the Fund Project of the Key Laboratory of Integrated Pest Management on Crops in South China,Ministry of Agriculture and Rural Affairs,China(SCIPM2018-05)+2 种基金the Key Research and Development Program of Nanning,China(20192065)the Guangdong Science and Technology Planning Project,China(2017A020216022)the Industrial Development Fund Support Project of Dapeng District,Shenzhen,China(KY20180117)。
文摘Mikania micrantha Kunth is an invasive alien weed and known as a plant killer around the world.Accurately and rapidly identifying M.micrantha in the wild is important for monitoring its growth status,as this helps management officials to take the necessary steps to devise a comprehensive strategy to control the invasive weed in the identified area.However,this approach still mainly depends on satellite remote sensing and manual inspection.The cost is high and the accuracy rate and efficiency are low.We acquired color images of the monitoring area in the wild environment using an Unmanned Aerial Vehicle(UAV)and proposed a novel network-MmNet-based on a deep Convolutional Neural Network(CNN)to identify M.micrantha in the images.The network consists of AlexNet Local Response Normalization(LRN),along with the GoogLeNet and continuous convolution of VGG inception models.After training and testing,the identification of 400 testing samples by MmNet is very good,with accuracy of 94.50%and time cost of 10.369 s.Moreover,in quantitative comparative analysis,the proposed MmNet not only has high accuracy and efficiency but also simple construction and outstanding repeatability.Compared with recently popular CNNs,MmNet is more suitable for the identification of M.micrantha in the wild.However,to meet the challenge of wild environments,more M.micrantha images need to be acquired for MmNet training.In addition,the classification labels need to be sorted in more detail.Altogether,this research provides some theoretical and scientific basis for the development of intelligent monitoring and early warning systems for M.micrantha and other invasive species.
基金funded by Directorate of Research and Humanity Engagement (DRPM), Universitas Indonesia via grant “Hibah PITTA 2017” with No. 328/UN2.R3.1/HKP.05.00/ 2017
文摘Objective: To optimize the ionic liquid based microwave-assisted extraction (IL-MAE) of polyphenolic content from Peperomia pellucida (L) Kunth. Methods: The IL-MAE factors as experimental design parameters, including microwave power, extraction time, ionic liquid concentration, and liquid–solid ratio had been involved. Response surface methodology and Box–Behnken design were used to obtain predictive model (multivariate quadratic regression equation) and optimization of the extraction process. The response surface was analyzed by using the yields of total polyphenolic content as response value. Results: Based on the obtained results the optimum extraction condition, including microwave power of 30% Watts, extraction time of 18.5 min, the ionic liquid concen-tration of 0.79 mol/L, and the liquid–solid ratio of 10.72 mL/g 1-Buthyl-3-methylimidazolium tetrafluoroborate ([bmim]BF4) as a solvent was selected. The regression model was obtained to predicts the yields from Peperomia pellucida:Y = 30.250 – 1.356X1 + 2.655X2 + 2.252X3 – 0.565X4 + 0.990 X1X3 – 8.172 X1X4 – 3.439 X3X4 – 4.178 X12 – 3.210 X32 – 6.786 X42 – 7.290 X12X3 + 5.575 X1X32 – 4.843 X32X4 with R2 = 0.82519. Scale-up confirmation test was obtained the maximum yields of total polyphenolics content with the amount of 31.1725μg GAE/g. Conclusions: The IL-MAE method produced a higher extraction polyphenolic and performed rapidly, easily and efficiently.
基金supported by grants from the National Natural Science Foundation of China(No. 81001694)
文摘Two new isospirostanol sapogenins named （25R）-5/%spirostane-（1αα 3a）-diol （1） and （25R）-5/%spirostane-（1α, 2α, 3α, 4α）- tetrol （2） were isolated from the whole herb ofReineckia carnea （Andr.） Kunth. The structures of the sapogenins were elucidated by chemical and spectral methods. methods
基金supported by grant “Hibah Tugas Akhir Mahasiswa Doktor(TADOK)Tahun 2018” Directorate of Research and Humanity Engagement Universitas Indonesia(grant number:1234/UN2.R3.1/HKP.05.00/2018)
文摘Objective:To isolate,identify,and evaluate a new angiotensin-converting enzyme inhibitor from Peperomia pellucida(L.)Kunth herbs.Methods:A dried sample of Peperomia pellucida herb was successively macerated with n-hexane and ethyl acetate.The ethyl acetate extract solution was evaporated to obtain the crude extract.Vacuum liquid column chromatography and thin layer chromatography were performed to obtain two pure compounds.Then,both compounds were elucidated and identified using the spectroscopic method.Angiotensin-converting enzyme inhibitory activity studies of both compounds were determined using angiotensin-converting enzyme kit WST-1 with spectrophotometer microplate reader 96-well at 450 nm wavelength.Results:Two bioactive compounds were successfully isolated from Peperomia pellucida herb,including a new compound of 2,3,5-trimethoxy-9-(12,14,15-trimethoxybenzyl)-1 H-indene and pellucidin A.Both compounds demonstrated angiotensin-converting enzyme inhibitory activity,with IC50 values of 72 μM(27.95 μg/mL)and 1 1μM(4.4 μg/mL),respectively.Conclusions:In the present study,two active angiotensin-converting enzyme inhibitors were successfully isolated and purified from Peperomia pellucida which is used as an antihypertensive in traditional medicine,and support its use as an angiotensin-converting enzyme-inhibiting drug.
文摘[Objective] The control methods of the bulb rot of Iphigenia indica Kunth.caused by Fusarium orthoceras App.et Wr.var.longius （Sherb.） were explored.[Method] The effect of eight different kinds of metal ions on the pathogen was studied;the contents of seven different kinds of metal ions in rhizosphere soil and bulb were also determined.[Result] The results showed that when the concentration of Cu2＋ or Mg2＋ was greater than 1.0mg/L or the concentration of Zn2＋ or Ca2＋ was lower than 0.5mg/L,the growth of pathogen could be inhibited.The results also showed that the bulb could enrich K highly,enrich Zn and Mg,limit the absorption of Ca and Fe,and inhibit the absorption of Cu and Mn during the growth process of bulb in I.indica Kunth.[Conclusion] Micronutrient fertilizer containing Mg could be used to prevent and cure the bulb rot of I.indica Kunth.
文摘Piper auritum Kunth,a member of the Piperacea family,is a small shrub native to the Central American tropics.This plant was traditionally used in Latin American countries for the treatment of various medical conditions.Increase in the recent studies of P.auritum’s chemical components leads to the isolation of monoterpenoids,sesquiterpenoids,phenylpropanoids and several other compounds.Previous research has revealed that these secondary metabolites have diverse pharmacological activities including antidiabetic,antioxidant,anti-inflammatory,anti-pathogeny microorganism and repellent activity.This review summarizes relevant findings on P.auritum,and hopes to contribute to the exploration of its potentials for further development as an alternative medicine.
