Using Kuala pear leaves and cortexes as materials, the total RNA was extracted using two methods and these two methods were compared. The most suited methods for Kuala pear were screened; and biotin-labeled cDNA probe...Using Kuala pear leaves and cortexes as materials, the total RNA was extracted using two methods and these two methods were compared. The most suited methods for Kuala pear were screened; and biotin-labeled cDNA probe was synthesized using RT-PCR. The main factors that affected the sensitivity of hybridization were studied. Studies indicated that the highest sensitivity was obtained under the following conditions： probe concentration 400 ng mL^-1, formamide concentration 45%, temperature 42℃, hybridization time 6 hours. The best hybridization results were obtained when the nitrocellulose membrane purchased from Gelman was used. Better blocking of hybridization was obtained using Tween 20 compared with albumin in bovine. The detection of the total RNA using different tissues and different extraction methods was compared. This study indicates that the total RNA of fresh leaf, old leaf, cortexes, and frozen leaf showed signs of hybridization using the two extraction methods.展开更多
1 Aging in Asia-an avalanche starts to form With decreasing fertilityrate and increasing life expectan-cy, the world is aging- withhighest rates in Asia. The socio-economic, financial and medicalconsequences of the ag...1 Aging in Asia-an avalanche starts to form With decreasing fertilityrate and increasing life expectan-cy, the world is aging- withhighest rates in Asia. The socio-economic, financial and medicalconsequences of the aging popu-展开更多
This paper will discuss the computerised developm en t control and approval system being developed for the Planning and Development C ontrol Department, City Hall of Kuala Lumpur, with stress on the GIS architectur e ...This paper will discuss the computerised developm en t control and approval system being developed for the Planning and Development C ontrol Department, City Hall of Kuala Lumpur, with stress on the GIS architectur e developed within the system. The prospects and challenges towards implementati on of the system are also discussed.展开更多
Urban areas usually experience higher temperatures compared with their rural surroundings; this is characterized as urban heat islands （UHIs）, which are the result of environmental overheating due to anthropic activ...Urban areas usually experience higher temperatures compared with their rural surroundings; this is characterized as urban heat islands （UHIs）, which are the result of environmental overheating due to anthropic activities. Urban areas,which are characterised by massive construction that reduce local vegetation coverage, are subject to the absorption of a large amount of solar radiation,which is only partially released into the atmosphere by radiation in the thermal infrared.展开更多
基金supported by the National Natural Science Foundation of China(30060053).
文摘Using Kuala pear leaves and cortexes as materials, the total RNA was extracted using two methods and these two methods were compared. The most suited methods for Kuala pear were screened; and biotin-labeled cDNA probe was synthesized using RT-PCR. The main factors that affected the sensitivity of hybridization were studied. Studies indicated that the highest sensitivity was obtained under the following conditions： probe concentration 400 ng mL^-1, formamide concentration 45%, temperature 42℃, hybridization time 6 hours. The best hybridization results were obtained when the nitrocellulose membrane purchased from Gelman was used. Better blocking of hybridization was obtained using Tween 20 compared with albumin in bovine. The detection of the total RNA using different tissues and different extraction methods was compared. This study indicates that the total RNA of fresh leaf, old leaf, cortexes, and frozen leaf showed signs of hybridization using the two extraction methods.
文摘1 Aging in Asia-an avalanche starts to form With decreasing fertilityrate and increasing life expectan-cy, the world is aging- withhighest rates in Asia. The socio-economic, financial and medicalconsequences of the aging popu-
文摘This paper will discuss the computerised developm en t control and approval system being developed for the Planning and Development C ontrol Department, City Hall of Kuala Lumpur, with stress on the GIS architectur e developed within the system. The prospects and challenges towards implementati on of the system are also discussed.
基金funded by Grand Challenge-SUS(Sustainability Science)Grants GC002C-15SUS and GC002A-15SUS
文摘Urban areas usually experience higher temperatures compared with their rural surroundings; this is characterized as urban heat islands （UHIs）, which are the result of environmental overheating due to anthropic activities. Urban areas,which are characterised by massive construction that reduce local vegetation coverage, are subject to the absorption of a large amount of solar radiation,which is only partially released into the atmosphere by radiation in the thermal infrared.
