Alzheimer’s disease not only affects the brain,but also induces metabolic dysfunction in peripheral organs and alters the gut microbiota.The aim of this study was to investigate systemic changes that occur in Alzhei...Alzheimer’s disease not only affects the brain,but also induces metabolic dysfunction in peripheral organs and alters the gut microbiota.The aim of this study was to investigate systemic changes that occur in Alzheimer’s disease,in particular the association between changes in peripheral organ metabolism,changes in gut microbial composition,and Alzheimer’s disease development.To do this,we analyzed peripheral organ metabolism and the gut microbiota in amyloid precursor protein-presenilin 1(APP/PS1)transgenic and control mice at 3,6,9,and 12 months of age.Twelve-month-old APP/PS1 mice exhibited cognitive impairment,Alzheimer’s disease-related brain changes,distinctive metabolic disturbances in peripheral organs and fecal samples(as detected by untargeted metabolomics sequencing),and substantial changes in gut microbial composition compared with younger APP/PS1 mice.Notably,a strong correlation emerged between the gut microbiota and kidney metabolism in APP/PS1 mice.These findings suggest that alterations in peripheral organ metabolism and the gut microbiota are closely related to Alzheimer’s disease development,indicating potential new directions for therapeutic strategies.展开更多
OBJECTIVE:To investigate effect of electroacupuncture(EA)with Bushen Jiannao on learning and memory ability in senescence-accelerated mouse prone 8(SAMP8)mice and the related mechanisms.METHODS:8-month-old senescence-...OBJECTIVE:To investigate effect of electroacupuncture(EA)with Bushen Jiannao on learning and memory ability in senescence-accelerated mouse prone 8(SAMP8)mice and the related mechanisms.METHODS:8-month-old senescence-accelerated-resistant(SAMR1)and SAMP8 mice were treated with EA at Baihui(GV 20),Shenshu(BL 23),and Taixi(KI 3)acupoint once a week for 8 weeks.The Morris water maze,enzyme linked immunosorbent assay,immunohistochemistry,and Western blot were used to assess Alzheimer’s disease(AD)-associated cognitive and neuroinflammatory phenotypes.RESULTS:Our data showed that EA treatment decreased activation of microglia and astrocytes,decreased levels of inflammatory factors including tumor necrosis factor-α(TNF-α),interleukin(IL)-6,and IL-17,and improved spatial memory deficits in SAMP8 mice.EA therapy with Bushen Jiannao exhibited anti-inflammatory properties and improved cognitive function.CONCLUSION:The present study indicates that EA treatment based on the interaction between kidney and brain can improve learning and memory ability by inhibiting activation of astrocytes and microglia and decreasing expression of pro-inflammatory cytokines,TNF-αand IL-17.EA treatment based on the interaction between kidney and brain may be an effective treatment for AD.展开更多
基金This work was supported by Key Project of Shanghai Science and Technology Committee(No.05JC14056)and Postdoctoral Fellowship of Shanghai Science and Technology Committee(No.05R214139).
基金financially supported by the National Natural Science Foundation of China,No.823 74552 (to WP)the Science and Technology Innovation Program of Hunan Province,No.2022RC1220 (to WP)+1 种基金the Natural Science Foundation of Hunan Province of China,Nos.2020JJ4803 (to WP),2022JJ40723 (to MY)the Scientific Research Launch Project for New Employees of the Second Xiangya Hospital of Central South University (to MY)
文摘Alzheimer’s disease not only affects the brain,but also induces metabolic dysfunction in peripheral organs and alters the gut microbiota.The aim of this study was to investigate systemic changes that occur in Alzheimer’s disease,in particular the association between changes in peripheral organ metabolism,changes in gut microbial composition,and Alzheimer’s disease development.To do this,we analyzed peripheral organ metabolism and the gut microbiota in amyloid precursor protein-presenilin 1(APP/PS1)transgenic and control mice at 3,6,9,and 12 months of age.Twelve-month-old APP/PS1 mice exhibited cognitive impairment,Alzheimer’s disease-related brain changes,distinctive metabolic disturbances in peripheral organs and fecal samples(as detected by untargeted metabolomics sequencing),and substantial changes in gut microbial composition compared with younger APP/PS1 mice.Notably,a strong correlation emerged between the gut microbiota and kidney metabolism in APP/PS1 mice.These findings suggest that alterations in peripheral organ metabolism and the gut microbiota are closely related to Alzheimer’s disease development,indicating potential new directions for therapeutic strategies.
基金Supported by National Key Research and Development Program—Research on Modernization of Traditional Chinese Medicine(Study on the Core Pathogenesis,Dialectical Elements and Syndrome Differentiation Standard of Kidney-Yang Deficiency Syndrome,No.2018YFC1704301)
文摘OBJECTIVE:To investigate effect of electroacupuncture(EA)with Bushen Jiannao on learning and memory ability in senescence-accelerated mouse prone 8(SAMP8)mice and the related mechanisms.METHODS:8-month-old senescence-accelerated-resistant(SAMR1)and SAMP8 mice were treated with EA at Baihui(GV 20),Shenshu(BL 23),and Taixi(KI 3)acupoint once a week for 8 weeks.The Morris water maze,enzyme linked immunosorbent assay,immunohistochemistry,and Western blot were used to assess Alzheimer’s disease(AD)-associated cognitive and neuroinflammatory phenotypes.RESULTS:Our data showed that EA treatment decreased activation of microglia and astrocytes,decreased levels of inflammatory factors including tumor necrosis factor-α(TNF-α),interleukin(IL)-6,and IL-17,and improved spatial memory deficits in SAMP8 mice.EA therapy with Bushen Jiannao exhibited anti-inflammatory properties and improved cognitive function.CONCLUSION:The present study indicates that EA treatment based on the interaction between kidney and brain can improve learning and memory ability by inhibiting activation of astrocytes and microglia and decreasing expression of pro-inflammatory cytokines,TNF-αand IL-17.EA treatment based on the interaction between kidney and brain may be an effective treatment for AD.
