Background:Chinese herbal medicines have been proven to be effective in clinical treatment and disease prevention.Jinlingzi San is a herbal medicine used for the treatment of endometriosis.However,a comprehensive netw...Background:Chinese herbal medicines have been proven to be effective in clinical treatment and disease prevention.Jinlingzi San is a herbal medicine used for the treatment of endometriosis.However,a comprehensive network pharmacology approach has not been used to understand the active ingredients and therapeutic mechanisms behind Jinlingzi San in the treatment of endometriosis.Methods:A network pharmacology approach and a molecular docking approach were used to predict the active ingredients and potential targets of Jinlingzi San in the treatment of endometriosis.Results:The integrated network pharmacology approach successfully identified 60 active ingredients and 540 key targets of action in Jinlingzi San dispersion.Among them,STAT3,TP53,MAPK3,RELA,MAPK1,JUN and other key genes were mainly regulated through pathways in cancer,lipid and atherosclerosis,AGE-RAGE signaling pathway in diabetic complications,pancreatic cancer,prostate cancer,PI3K-Akt signaling pathway,Th17 cell differentiation,and other signaling pathways in the cell body,receptor complexes,cytoplasm,nucleoplasm,and plasma membrane.Conclusion:Based on a systems network pharmacology approach,our work successfully predicts the active components and potential targets of Jinlingzi San for the treatment of endometriosis and helps to illustrate the mechanism of action at an integrative level.This demonstrates that Jinlingzi San for the treatment of endometriosis is regulated through a multi-component,multi-target,multi-system co-regulation.This study identifies key genes and pathways associated with endometriosis and pathogenesis from new insights,and also provides a viable approach to the chemical basis and pharmacological studies of Jinlingzi San.展开更多
基金supported by grants from the Heilongjiang Provincial Traditional Chinese Medicine Research Project:Mechanism study of Zhuyu Ning in treating endometriosis based on the Wnt/β-catenin signaling pathway (ZHY2022-130)the National Traditional Chinese Medicine Clinical Research Base Business Construction Scientific Research Special Project (JDZX2015057)of the State Administration of Traditional Chinese Medicine:Clinical observation of the therapeutic effect of acupuncture on dysmenorrhea in endometriosis based on the VEGF/VEGFR pathway.
文摘Background:Chinese herbal medicines have been proven to be effective in clinical treatment and disease prevention.Jinlingzi San is a herbal medicine used for the treatment of endometriosis.However,a comprehensive network pharmacology approach has not been used to understand the active ingredients and therapeutic mechanisms behind Jinlingzi San in the treatment of endometriosis.Methods:A network pharmacology approach and a molecular docking approach were used to predict the active ingredients and potential targets of Jinlingzi San in the treatment of endometriosis.Results:The integrated network pharmacology approach successfully identified 60 active ingredients and 540 key targets of action in Jinlingzi San dispersion.Among them,STAT3,TP53,MAPK3,RELA,MAPK1,JUN and other key genes were mainly regulated through pathways in cancer,lipid and atherosclerosis,AGE-RAGE signaling pathway in diabetic complications,pancreatic cancer,prostate cancer,PI3K-Akt signaling pathway,Th17 cell differentiation,and other signaling pathways in the cell body,receptor complexes,cytoplasm,nucleoplasm,and plasma membrane.Conclusion:Based on a systems network pharmacology approach,our work successfully predicts the active components and potential targets of Jinlingzi San for the treatment of endometriosis and helps to illustrate the mechanism of action at an integrative level.This demonstrates that Jinlingzi San for the treatment of endometriosis is regulated through a multi-component,multi-target,multi-system co-regulation.This study identifies key genes and pathways associated with endometriosis and pathogenesis from new insights,and also provides a viable approach to the chemical basis and pharmacological studies of Jinlingzi San.
