Metallothioneins （MTs） were discovered in 1957 by Margoshes and Vallee and identified as low-molecular weight and sulphydryl rich proteins. It is not surprising that most mammalian tissues contain age related basal ...Metallothioneins （MTs） were discovered in 1957 by Margoshes and Vallee and identified as low-molecular weight and sulphydryl rich proteins. It is not surprising that most mammalian tissues contain age related basal levels of MTs since they are involved in metalloregulatory processes that include cell growth and multiplication. In an effort to understand the biology of this intriguing tumor, various biomarkers such as oncogenes, p53 tumor suppressor gene, war 1 protein, proliferating cell nuclear antigen, telomerase, microsatellite markers and cytogenetic changes have been examined. One biomarker which has recently shown to be expressed in various human tumors but still less reported in carcinoma is MT. Immunohistochemical detection of MT proteins in cold acetone-fixed paraffin embedded liver sections was performed by the streptavidin-avidin-biotin immunoperoxidase complex method.展开更多
目的:探究4℃冷藏过程中鲈鱼肌肉品质的变化。方法:测定4℃冷藏过程中鲈鱼肌肉pH值、菌落总数、总挥发性盐基氮(total volatile base nitrogen,TVB-N)含量、K值和质构等指标,综合评价鲈鱼生理生化品质的变化,采用Masson染色和免疫组化...目的:探究4℃冷藏过程中鲈鱼肌肉品质的变化。方法:测定4℃冷藏过程中鲈鱼肌肉pH值、菌落总数、总挥发性盐基氮(total volatile base nitrogen,TVB-N)含量、K值和质构等指标,综合评价鲈鱼生理生化品质的变化,采用Masson染色和免疫组化分析评价组织形态学变化,利用酶解荧光肽底物测定特异性酶解胶原蛋白的基质金属蛋白酶(matrix metalloproteinases,MMPs)活力变化。结果:鲈鱼在贮藏期间pH值呈先下降后升高趋势,菌落总数、TVB-N含量和K值随贮藏时间的延长而增加,并在第8天超过临界值。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析结果显示,贮藏至6 d鲈鱼肌肉蛋白组成没有明显变化,而在第8天肌球蛋白重链降解明显。Masson染色和I型胶原免疫组化分析结果显示,随贮藏时间的延长,肌内膜逐渐被降解,至第12天,肌膜I型胶原蛋白和肌纤维之间出现明显间隙。MMPs活力随贮藏时间的延长而明显增加,而鱼肉硬度不断下降。结论:鱼类冷藏期间肌肉的软化与胶原蛋白分解密切相关,而MMPs对I型胶原蛋白的降解可能是鱼死后肌肉软化的主要原因。展开更多
目的：探讨高分辨率 MRI 对颈动脉粥样硬化斑块性质、形态评估的准确性,以及影响斑块稳定性的病理学因素。方法对2013年2月—2015年2月聊城市人民医院103例颈动脉内膜剥脱术患者行前瞻性研究。术前均行颈动脉高分辨率 MRI 多序列扫描,...目的：探讨高分辨率 MRI 对颈动脉粥样硬化斑块性质、形态评估的准确性,以及影响斑块稳定性的病理学因素。方法对2013年2月—2015年2月聊城市人民医院103例颈动脉内膜剥脱术患者行前瞻性研究。术前均行颈动脉高分辨率 MRI 多序列扫描,术后对斑块切片行 HE 染色、油红 O 染色、Masson 三染以及 CD68免疫组织化学检测。根据病理结果,分为易损斑块组和稳定斑块组,并和 MRI 检测结果比较。结果103例颈动脉粥样硬化斑块中共有易损斑块42例、稳定斑块61例。与稳定斑块组比较,易损斑块组胶原含量明显降低（10.37%±3.19% vs 17.02%±4.56%）,而脂质含量则明显升高（19.14%±3.26% vs 13.99%±3.47%）,CD68表达明显增加,差异均有统计学意义（t =8.169、7.585, P 值均〈0.01）。与稳定斑块组比较,易损斑块组平均管腔面积、最小管腔面积、平均管壁面积、最大管壁面积、平均管壁厚度、最大管壁厚度、平均标准化管壁指数、最大标准化管壁指数均明显升高或降低,差异均有统计学意义（t =5.099、7.513、4.068、2.535、3.182、3.503、10.868、6.233, P 值均〈0.05）。与病理检查比较,MRI 检查颈动脉粥样硬化斑块中富含脂质的坏死核、斑块内出血、钙化检出率偏低,而纤维帽表面破裂检出率偏高,但差异均无统计学意义（P 值均〉0.05）。结论颈动脉粥样硬化易损斑块与稳定斑块在病理形态学方面存在显著差异,将高分辨率MR 多序列技术相结合,能更准确、更全面地测量其管壁结构、斑块成分以及体积,可用于斑块的风险评估。展开更多
The distribution of bursin in sacculus rotundus and gut-associated lymphoid tissues was investigated in rabbit by immuno-histochemical staining method with anti-bursin monoclonal antibody （McAb）2F9-4. The results in...The distribution of bursin in sacculus rotundus and gut-associated lymphoid tissues was investigated in rabbit by immuno-histochemical staining method with anti-bursin monoclonal antibody （McAb）2F9-4. The results indicated that the appearance of the bursin-containing cells coincided with the morphodifferentiation and growth process in rabbit. Bursin immunized cells not only distributed in dome epithelium and mucosal epithelium, but also in follicular germinal center, dome, T-cell area, goblet cell, inter-epithelial lymphocyte and cap-zone of sacculus rotundus. This founding suggested that bursincontaining cells in sacculus rotundus might play an important role in intestinal mucosal immunity.展开更多
The breast cancer is the most common cause of cancer death in women. To establish an early stage in situ imaging of breast cancer cells, green quantum dots （QDs） are used as a fluorescent signal generator. The QDs b...The breast cancer is the most common cause of cancer death in women. To establish an early stage in situ imaging of breast cancer cells, green quantum dots （QDs） are used as a fluorescent signal generator. The QDs based imaging of breast cancer cells involves anti-HER2/neu antibody for labeling the over expressed HER2 on the surface of breast cancer cells. The complete assay involves breast cancer cells, biotin labeled antibody and streptavidin conjugated QDs. The breast cancer cells are grown in culture plates and exposed to the biotin labeled antibodies, and then exposed to streptavidin labeled QDs to utilize the strong and stable biotin-streptavidin interaction. Fluorescent images of the complete assay for breast cancer cells are evaluated on a microscope with a UV light source. Results show that the breast cancer cells in the complete assay are used as fluorescent cells with brighter signals compared with those labeled by the organic dye using similar parameters and the same number of cells.展开更多
文摘Metallothioneins （MTs） were discovered in 1957 by Margoshes and Vallee and identified as low-molecular weight and sulphydryl rich proteins. It is not surprising that most mammalian tissues contain age related basal levels of MTs since they are involved in metalloregulatory processes that include cell growth and multiplication. In an effort to understand the biology of this intriguing tumor, various biomarkers such as oncogenes, p53 tumor suppressor gene, war 1 protein, proliferating cell nuclear antigen, telomerase, microsatellite markers and cytogenetic changes have been examined. One biomarker which has recently shown to be expressed in various human tumors but still less reported in carcinoma is MT. Immunohistochemical detection of MT proteins in cold acetone-fixed paraffin embedded liver sections was performed by the streptavidin-avidin-biotin immunoperoxidase complex method.
基金“十二五”国家科技支撑计划项目，山东省科技发展计划项目，山东省科技惠民计划项目(2014kjhm0109)@@@@National Science and Technology Infrastructure Program during the 12th Five-year Plan Period，Science and Technology Development Plan Project of Shandong Province，Science and Technology to Benefit the People Project of Shandong Province
文摘目的：探讨高分辨率 MRI 对颈动脉粥样硬化斑块性质、形态评估的准确性,以及影响斑块稳定性的病理学因素。方法对2013年2月—2015年2月聊城市人民医院103例颈动脉内膜剥脱术患者行前瞻性研究。术前均行颈动脉高分辨率 MRI 多序列扫描,术后对斑块切片行 HE 染色、油红 O 染色、Masson 三染以及 CD68免疫组织化学检测。根据病理结果,分为易损斑块组和稳定斑块组,并和 MRI 检测结果比较。结果103例颈动脉粥样硬化斑块中共有易损斑块42例、稳定斑块61例。与稳定斑块组比较,易损斑块组胶原含量明显降低（10.37%±3.19% vs 17.02%±4.56%）,而脂质含量则明显升高（19.14%±3.26% vs 13.99%±3.47%）,CD68表达明显增加,差异均有统计学意义（t =8.169、7.585, P 值均〈0.01）。与稳定斑块组比较,易损斑块组平均管腔面积、最小管腔面积、平均管壁面积、最大管壁面积、平均管壁厚度、最大管壁厚度、平均标准化管壁指数、最大标准化管壁指数均明显升高或降低,差异均有统计学意义（t =5.099、7.513、4.068、2.535、3.182、3.503、10.868、6.233, P 值均〈0.05）。与病理检查比较,MRI 检查颈动脉粥样硬化斑块中富含脂质的坏死核、斑块内出血、钙化检出率偏低,而纤维帽表面破裂检出率偏高,但差异均无统计学意义（P 值均〉0.05）。结论颈动脉粥样硬化易损斑块与稳定斑块在病理形态学方面存在显著差异,将高分辨率MR 多序列技术相结合,能更准确、更全面地测量其管壁结构、斑块成分以及体积,可用于斑块的风险评估。
基金Project supported by the National Natural Science Foundation of China （Grant No.39460060）
文摘The distribution of bursin in sacculus rotundus and gut-associated lymphoid tissues was investigated in rabbit by immuno-histochemical staining method with anti-bursin monoclonal antibody （McAb）2F9-4. The results indicated that the appearance of the bursin-containing cells coincided with the morphodifferentiation and growth process in rabbit. Bursin immunized cells not only distributed in dome epithelium and mucosal epithelium, but also in follicular germinal center, dome, T-cell area, goblet cell, inter-epithelial lymphocyte and cap-zone of sacculus rotundus. This founding suggested that bursincontaining cells in sacculus rotundus might play an important role in intestinal mucosal immunity.
基金Supported by the Foundation for Cultivating the Excellent Doctoral Dissertation of Jiangxi Province of China (YBP08A03)~~
文摘The breast cancer is the most common cause of cancer death in women. To establish an early stage in situ imaging of breast cancer cells, green quantum dots （QDs） are used as a fluorescent signal generator. The QDs based imaging of breast cancer cells involves anti-HER2/neu antibody for labeling the over expressed HER2 on the surface of breast cancer cells. The complete assay involves breast cancer cells, biotin labeled antibody and streptavidin conjugated QDs. The breast cancer cells are grown in culture plates and exposed to the biotin labeled antibodies, and then exposed to streptavidin labeled QDs to utilize the strong and stable biotin-streptavidin interaction. Fluorescent images of the complete assay for breast cancer cells are evaluated on a microscope with a UV light source. Results show that the breast cancer cells in the complete assay are used as fluorescent cells with brighter signals compared with those labeled by the organic dye using similar parameters and the same number of cells.
