摘要
目的:探讨射频消融(RFA)对兔肝VX2肿瘤血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响.方法:建立日本大耳白兔肝VX2肿瘤模型,随机分为3组:对照组直接处死取肝脏标本;RFA治疗(4h和24h)组于射频消融后4h和24h处死取出肝脏组织.并以正常兔肝脏组织为正常对照,通过免疫组织化学方法和逆转录聚合酶链式反应(RT-PCR)方法检测VEGF的蛋白和基因表达.结果:VX2肿瘤浸润性生长,射频后中央消融区呈现大片坏死,周围见炎性反应带,外围癌组织残留.免疫组化和RT-PCR示对照组肿瘤组织VEGF的蛋白和基因表达均高于正常组(VEGF/GAPDH基因灰度比:1.3048±0.1936vs0.8993±0.1107,P<0.05),射频消融后4h和24h表达明显下降(VEGF/GAPDH基因灰度比:0.7388±0.1503,0.8935±0.1936,均P<0.05),但不同时间段并无明显差异.结论:RFA治疗能下调肿瘤VEGF基因和蛋白水平的表达,抑制血管生成,减少血供,进而降低了复发和转移几率.
AIM: To investigate the effect of radio frequency ablation (RFA) on vascular endothelial growth factor (VEGF) expression in rabbit liver VX2 tumor models. METHODS: The liver VX2 tumor models were established by planting the tumor tissue suspen- sion into rabbits' liver and then divided into three groups: Control group were killed directly to obtain liver specimens; treat group were treated with RFA and executed after 4 h and 24 h randomly. And normal rabbit livers were used as the normal group. The expression of VEGF in all groups was detected by immunohistochemis- try and reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: VX2 liver tumor showed infiltrative growth. After RFA, large necrosis in the central ablation area, surrounded by inflammatory re- sponse belt and peripheral residual tumor tissue, was observed in the specimens from tumors. By immunohistochemistry and RT-PCR, the protein and gene expressions of VEGF in the control group were higher than those in the normal group (VEGF-mRNA/GAPDH-mRNA 1.3048 ± 0.1936 vs 0.8993 ± 0.1107, P 〈 0.05), while those in treatment group (4 h: 0.7388 ± 0.1503; 24 h: 0.8935±0.1936) were decreased obviously as compared with the control group (both P 〈 0.05). However, there was no significant difference in the expression of VEGF in the tumor tissue among the dif- ferent time points of the RFA group (P〉0.05). CONCLUSION: RFA treatment can effectively down-regulate the protein and gene expression of VEGF, inhibit the angiopoiesis and reduce the blood supply, thus may reduce tumor relapse and metastasis.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第8期780-784,共5页
World Chinese Journal of Digestology
基金
南通市社会发展基金资助项目
No.S2006032~~
关键词
射频消融
VX2肿瘤
血管内皮生长因子
免疫组化
Radio frequency ablation
VX2 liver tu-mor
Vascular endothelial growth factor
Immuno-histochemical