Objective To re-evaluate the potential toxicity of Danshen Injection(DI) in Beagle's dogs by repeated iv injection.Methods DI was iv given to the dogs at the doses of 0,1.6,5.4,and 16.0 g/(kg·d)(4 per sex per...Objective To re-evaluate the potential toxicity of Danshen Injection(DI) in Beagle's dogs by repeated iv injection.Methods DI was iv given to the dogs at the doses of 0,1.6,5.4,and 16.0 g/(kg·d)(4 per sex per group) for 13weeks.During the test period,the clinical signs,mortality,body weights,food consumption,rectal temperature,ophthalmoscopy,electrocardiography,urinalysis,hematology,serum biochemistry,organ weights,gross findings,and histopathology were examined.Results Dogs iv given with DI at the doses of 0,1.6,5.4,and 16.0 g/(kg·d) for13 weeks had no drug-related changes in mortality,body weight,food consumption,temperature,electrocardiography,ophthalmoscopy,urinalysis parameters,and organ weights.The hematological parameter data showed a significant decrease in red blood cells and hemoglobin concentration in the high-dose group and a significant increase in activated partial thromboplastin time suggesting an effect on haemopoiesis.For biochemical parameters,a significant decrease in glucose and a significant increase in total bilirubin were observed in the high-dose group,and the latter was considered to be toxicologically insignificant as lack of histopathological correlate.However,the histopathological examinations of the injection site showed that DI could cause dose-dependent focal inflammation.Conclusion That the iv injection with DI into dogs at 16 g/(kg·d) for 13 weeks could cause the decreases in red blood cell parameters and glucose,as well as the lesions of the injection site.The no observed adverse effect level is5.4 g/(kg·d),which suggests that safe clinical dosing be possible.展开更多
目的:观察银杏内酯氯化钠注射液长期给药对犬的各种毒性反应,以评价其安全性。方法:银杏内酯氯化钠注射液0、37.5、75、150mg/kg 每 d 静脉给药1次,每周6d,连续给药13周,观察给药期间动物的一般状况、体重变化、摄食量、体温、尿、粪便...目的:观察银杏内酯氯化钠注射液长期给药对犬的各种毒性反应,以评价其安全性。方法:银杏内酯氯化钠注射液0、37.5、75、150mg/kg 每 d 静脉给药1次,每周6d,连续给药13周,观察给药期间动物的一般状况、体重变化、摄食量、体温、尿、粪便、心电图、血液细胞学及生化学指标、大体解剖及进行组织病理学检查。结果:Beagle 犬静脉注射银杏内酯氯化钠注射液13周,各剂量组动物行为活动均如常,一般情况良好。高、中、低剂量组心电图、犬血液学和血清生化学指标均未见明显与受试物相关的异常改变。给药组动物的脏器重量及其系数与对照组比较未见与给药相关的显著性差异,系统尸检和病理切片结果显示,各受检脏器均未见明显与给药相关的病理改变。结论:在本试验条件下,银杏内酯氯化钠注射液连续静脉给药13周后,犬的无毒剂量为150mg/kg(相当于推荐临床人用剂量100mg/60kg 人/d 的90倍)。展开更多
Objective To investigate the chemical constituents from Aidi Injection.Methods The chemical constituents were isolated by chromatography on Sephadex LH-20 gel columns and reverse phase semi-preparative HPLC repeatedly...Objective To investigate the chemical constituents from Aidi Injection.Methods The chemical constituents were isolated by chromatography on Sephadex LH-20 gel columns and reverse phase semi-preparative HPLC repeatedly.Their structures were identified by spectroscopic analysis(NMR and MS).Results Twenty-two compounds were isolated and identified to be 3-O-3′,4′-diacetyl-β-D-xylopyranosyl-6-O-β-D-glucopyranosyl- cycloastragenol(1),astragaloside IV(2),astragaloside II(3),astragaloside I(4),isoastragaloside I(5), acetylastragaloside I(6),ginsenosid Re(7),ginsenoside Rf(8),ginsenoside Rg1(9),ginsenoside Rb3(10), notoginsenoside R4(11),ginsenoside Rb1(12),ginsenoside Rc(13),ginsenoside Rb2(14),ginsenoside Rd(15), lucyoside H(16),3-O-β-D-glucopyranosyl(1→4)-β-D-glucopyranosyl(1→3)-α-L-rhamnopyranosyl(1→2)-α-L- arabinopyranosyl oleanolic acid 28-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside (17),3-O-β-D-glucopyranosyl(1→3)-α-L-rhamnopyranosyl[β-D-glucopyranosyl-(1→4)]-(1→2)-α-L-arabinopyranosyl oleanolic acid 28-O-α-L-arabinopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside(18),syringin (19),elentheroside E(20),4-(1,2,3-trihydroxypropyl)-2,6-dimethoxyphenyl-1-O-β-D-glucopyranoside(21),and coniferin(22).Conclusion Compounds 1-6 are originated from Astragalus membranceus,compounds 7-18 are originated from Panax ginseng,and compounds 19-22 are originated from Acanthopanax senticosus by LC-MS analysis.Compound 1 is a new compound.展开更多
A new flow injection chemiluminescence method for the determination of paeonol was established.The method was based on chemiluminescence reaction of paeonol with Ce（Ⅳ） in sulfuric acid condition sensitized by rhoda...A new flow injection chemiluminescence method for the determination of paeonol was established.The method was based on chemiluminescence reaction of paeonol with Ce（Ⅳ） in sulfuric acid condition sensitized by rhodamine 6G.The method is simple,convenient and sensitive.The linear relationship between the intensity of chemiluminescence and the concentration of paeonol is over the range of 5.0×10-7 ～1.0×10-4mol/L.The detection limit is 9.7×10-8 mol/L,the relative standard deviation is 1.8 % for 7 measurements of 1.0×10-5mol/L standard solution.This method has been successfully applied to the determination of paeonol in ointment with recovery between 98.8% and 104%,and the results were in good agreement with those obtained by UV method.展开更多
基金National Natural Science Foundation of China(81173540)
文摘Objective To re-evaluate the potential toxicity of Danshen Injection(DI) in Beagle's dogs by repeated iv injection.Methods DI was iv given to the dogs at the doses of 0,1.6,5.4,and 16.0 g/(kg·d)(4 per sex per group) for 13weeks.During the test period,the clinical signs,mortality,body weights,food consumption,rectal temperature,ophthalmoscopy,electrocardiography,urinalysis,hematology,serum biochemistry,organ weights,gross findings,and histopathology were examined.Results Dogs iv given with DI at the doses of 0,1.6,5.4,and 16.0 g/(kg·d) for13 weeks had no drug-related changes in mortality,body weight,food consumption,temperature,electrocardiography,ophthalmoscopy,urinalysis parameters,and organ weights.The hematological parameter data showed a significant decrease in red blood cells and hemoglobin concentration in the high-dose group and a significant increase in activated partial thromboplastin time suggesting an effect on haemopoiesis.For biochemical parameters,a significant decrease in glucose and a significant increase in total bilirubin were observed in the high-dose group,and the latter was considered to be toxicologically insignificant as lack of histopathological correlate.However,the histopathological examinations of the injection site showed that DI could cause dose-dependent focal inflammation.Conclusion That the iv injection with DI into dogs at 16 g/(kg·d) for 13 weeks could cause the decreases in red blood cell parameters and glucose,as well as the lesions of the injection site.The no observed adverse effect level is5.4 g/(kg·d),which suggests that safe clinical dosing be possible.
基金National Technology Research Program for Creating New Drugs (2009ZX09308-003 and 2011ZX09201-201-16)
文摘Objective To investigate the chemical constituents from Aidi Injection.Methods The chemical constituents were isolated by chromatography on Sephadex LH-20 gel columns and reverse phase semi-preparative HPLC repeatedly.Their structures were identified by spectroscopic analysis(NMR and MS).Results Twenty-two compounds were isolated and identified to be 3-O-3′,4′-diacetyl-β-D-xylopyranosyl-6-O-β-D-glucopyranosyl- cycloastragenol(1),astragaloside IV(2),astragaloside II(3),astragaloside I(4),isoastragaloside I(5), acetylastragaloside I(6),ginsenosid Re(7),ginsenoside Rf(8),ginsenoside Rg1(9),ginsenoside Rb3(10), notoginsenoside R4(11),ginsenoside Rb1(12),ginsenoside Rc(13),ginsenoside Rb2(14),ginsenoside Rd(15), lucyoside H(16),3-O-β-D-glucopyranosyl(1→4)-β-D-glucopyranosyl(1→3)-α-L-rhamnopyranosyl(1→2)-α-L- arabinopyranosyl oleanolic acid 28-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside (17),3-O-β-D-glucopyranosyl(1→3)-α-L-rhamnopyranosyl[β-D-glucopyranosyl-(1→4)]-(1→2)-α-L-arabinopyranosyl oleanolic acid 28-O-α-L-arabinopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside(18),syringin (19),elentheroside E(20),4-(1,2,3-trihydroxypropyl)-2,6-dimethoxyphenyl-1-O-β-D-glucopyranoside(21),and coniferin(22).Conclusion Compounds 1-6 are originated from Astragalus membranceus,compounds 7-18 are originated from Panax ginseng,and compounds 19-22 are originated from Acanthopanax senticosus by LC-MS analysis.Compound 1 is a new compound.
文摘A new flow injection chemiluminescence method for the determination of paeonol was established.The method was based on chemiluminescence reaction of paeonol with Ce（Ⅳ） in sulfuric acid condition sensitized by rhodamine 6G.The method is simple,convenient and sensitive.The linear relationship between the intensity of chemiluminescence and the concentration of paeonol is over the range of 5.0×10-7 ～1.0×10-4mol/L.The detection limit is 9.7×10-8 mol/L,the relative standard deviation is 1.8 % for 7 measurements of 1.0×10-5mol/L standard solution.This method has been successfully applied to the determination of paeonol in ointment with recovery between 98.8% and 104%,and the results were in good agreement with those obtained by UV method.
