The Neospora caninum immune mapped protein 1(Nc IMP1) was identified as a membrane protein,and a previous study indicated that Nc IMP1 could be a promising vaccine candidate against neosporosis. In this study, the imm...The Neospora caninum immune mapped protein 1(Nc IMP1) was identified as a membrane protein,and a previous study indicated that Nc IMP1 could be a promising vaccine candidate against neosporosis. In this study, the immune response and protection efficacy of Nc IMP1 were evaluated. The coding sequence of Nc IMP1 was inserted into the eukaryotic expression vector pc DNA3.1(+), resulting in the recombination plasmid pc DNAIMP1, which was used for the intramuscular immunization of BALB/c mice. After immunization, the immune response was evaluated using a lymphoproliferative assay and cytokine and antibody measurements. Quantification of the cerebral parasite burden of mice challenged with 2106 N. caninum was performed 14 days after the last immunization. The results showed that the mice immunized with pc DNA-IMP1 developed a high level of specific antibody responses against recombinant Nc IMP1,with a mixed Ig G1/Ig G2 a response and a predominance of Ig G2 a production. The cellular immune response was associated with the production of IFN-γ, IL-2, IL-4 and IL-10 cytokines. The experiment was terminated 30 days p.i.,and the cerebral parasite burden in each mouse was assessed by quantitative PCR. The parasite burden was significantly reduced in the pc DNA-IMP1-vaccinated mice. These data suggest that IMP1 is a promising vaccine candidate against neosporosis.展开更多
目的:探讨重症监护病房(intensive care unit,ICU)患者铜绿假单胞菌IMP-1耐药基因检测及耐药性,为临床合理使用抗生素提供依据。方法:采用聚合酶链反应(PCR)检测耐药基因IMP-1,ATB Expression自动鉴定病原菌及药敏分析。结果:2007～200...目的:探讨重症监护病房(intensive care unit,ICU)患者铜绿假单胞菌IMP-1耐药基因检测及耐药性,为临床合理使用抗生素提供依据。方法:采用聚合酶链反应(PCR)检测耐药基因IMP-1,ATB Expression自动鉴定病原菌及药敏分析。结果:2007～2009年共分离出106株铜绿假单胞菌,对头孢他啶、亚胺培南、哌拉西林、氨曲南、头孢哌酮、头孢吡肟、左氧氟沙星的耐药率分别为50.9%、39.6%、79.2%、56.6%、57.5%、41.5%、25.5%。每年耐亚胺培南株的检出率依序分别为16.0%、28.3%、39.6%。耐亚胺培南株绝大部分检出IMP耐药基因。结论:ICU患者金属酶表型阳性菌株检出的基因型主要为IMP-1,产酶菌株耐药性强,随时进行细菌耐药性监测,正确使用有效的抗生素是控制感染和延缓细菌耐药的关键。展开更多
基金supported by National Key Basic Research Program of China(2015CB150300)Beijing Municipal Natural Science Foundation(6131001)
文摘The Neospora caninum immune mapped protein 1(Nc IMP1) was identified as a membrane protein,and a previous study indicated that Nc IMP1 could be a promising vaccine candidate against neosporosis. In this study, the immune response and protection efficacy of Nc IMP1 were evaluated. The coding sequence of Nc IMP1 was inserted into the eukaryotic expression vector pc DNA3.1(+), resulting in the recombination plasmid pc DNAIMP1, which was used for the intramuscular immunization of BALB/c mice. After immunization, the immune response was evaluated using a lymphoproliferative assay and cytokine and antibody measurements. Quantification of the cerebral parasite burden of mice challenged with 2106 N. caninum was performed 14 days after the last immunization. The results showed that the mice immunized with pc DNA-IMP1 developed a high level of specific antibody responses against recombinant Nc IMP1,with a mixed Ig G1/Ig G2 a response and a predominance of Ig G2 a production. The cellular immune response was associated with the production of IFN-γ, IL-2, IL-4 and IL-10 cytokines. The experiment was terminated 30 days p.i.,and the cerebral parasite burden in each mouse was assessed by quantitative PCR. The parasite burden was significantly reduced in the pc DNA-IMP1-vaccinated mice. These data suggest that IMP1 is a promising vaccine candidate against neosporosis.
文摘目的:探讨重症监护病房(intensive care unit,ICU)患者铜绿假单胞菌IMP-1耐药基因检测及耐药性,为临床合理使用抗生素提供依据。方法:采用聚合酶链反应(PCR)检测耐药基因IMP-1,ATB Expression自动鉴定病原菌及药敏分析。结果:2007～2009年共分离出106株铜绿假单胞菌,对头孢他啶、亚胺培南、哌拉西林、氨曲南、头孢哌酮、头孢吡肟、左氧氟沙星的耐药率分别为50.9%、39.6%、79.2%、56.6%、57.5%、41.5%、25.5%。每年耐亚胺培南株的检出率依序分别为16.0%、28.3%、39.6%。耐亚胺培南株绝大部分检出IMP耐药基因。结论:ICU患者金属酶表型阳性菌株检出的基因型主要为IMP-1,产酶菌株耐药性强,随时进行细菌耐药性监测,正确使用有效的抗生素是控制感染和延缓细菌耐药的关键。
