摘要
目的研究欧前胡素衍生物(IMP-1)对肺癌A549细胞的增殖抑制作用及其初步机制。方法使用四甲基偶氮唑盐(MTT)法考察IMP-1对肺癌A549细胞的增殖抑制作用,采用流式细胞术检测IMP-1对细胞凋亡及细胞周期的影响,通过蛋白免疫印迹(Western blot)法考察IMP-1对周期相关蛋白和凋亡相关蛋白表达的影响。结果IMP-1对肺癌A549细胞的增殖具有良好的抑制效果,其半数抑制浓度为3.62μmol/L;IMP-1通过下调细胞周期蛋白D1、细胞周期蛋白E、细胞周期蛋白依赖性激酶——细胞分裂周期基因2蛋白表达量阻滞细胞周期于G1期;通过下调抑凋亡蛋白——髓样细胞白血病蛋白-1、B淋巴细胞瘤-2表达水平,上调促凋亡蛋白——B淋巴细胞瘤-2相关X蛋白表达水平,从而诱导A549细胞凋亡,进一步抑制细胞生长。结论IMP-1通过阻滞A549细胞于G1期及诱导细胞凋亡,从而抑制肺癌A549细胞的增殖。
Objective To study the inhibitory effect of imperatorin derivative IMP-1 on lung cancer A549 cells growth and its preliminary mechanism.Methods The methyl thiazolyl tetrazolium(MTT)salt method was used to investigate the inhibitory effect of IMP-1 on lung cancer A 549 cells growth;the effect of IMP-1 on the cellular apoptosis and cell cycle was detected by flow cytometry;the effect of IMP-1 on the expression of cell cycle-related proteins and apoptosis-related proteins was investigated by Western blot.Results IMP-1 had good inhibiting effect on the proliferation of A549 cells,and the IC50 value was 3.62μmol/L.IMP-1 could downregulate the expression amounts of CyclinD1,CyclinE and cell cycle-dependent kinase CDC2,thereby arrested the cell cycle at G1 phase.Also,IMP-1 downregulated the expression levels of anti-apoptotic proteins Mcl-1 and Bcl-2,and upregulated the expression level of pro-apoptotic proteins Bax,thus induced apoptosis of A549 cells and further inhibied the cell growth.Conclusion IMP-1 inhibits the proliferation of lung cancer A549 cells by arresting A549 cells at G1 phase and inducing the cellular apoptosis.
作者
杨晓琪
毛景涛
刘娟
马维娜
YANG Xiaoqi;MAO Jingtao;LIU Juan;MA Weina(Department of Thoracic Surgery,Shaanxi Provincial Forest Industry Worker’s Hospital,Xi′an,Shaanxi 710300,China;Department of Oncology,521 Hospital of Weaponry Industry,Xi′an,Shaanxi 710065,China;School of Pharmacy,Health Science Center,Xi′an Jiaotong University,Xi′an,Shaanxi 710061,China)
出处
《重庆医学》
CAS
2022年第13期2172-2176,2183,共6页
Chongqing medicine
基金
国家自然科学基金项目(81603148)。
