The polysaccharide extracted from Huidouba（PEH） exihibited a significant anti-diabetic activity. The polysaccharide contained glucose, mannose and galactose in a content ratio of 1.00：0.34：0.32. After administrati...The polysaccharide extracted from Huidouba（PEH） exihibited a significant anti-diabetic activity. The polysaccharide contained glucose, mannose and galactose in a content ratio of 1.00：0.34：0.32. After administration with 50-- 200 mg/kg body weight of PEH to type II diabetic mice induced by alloxan for four weeks, the blood glucose level of mice was decreased significantly. Moreover, the SOD activity increased at the same time（p〈0.01）. PEH showed a significant anti-diabetic and anti-oxidation activity. In addition, PEH affected the BUN and ALT levels（p〈0.05）, which are important characteristics of diabetes mellitus. Through this work, PEH should be a potential anti-diabetic drug in the future.展开更多
Objective:Huidouba(HDB) is a Chinese folk medicine used to treat diabetes in Sichuan Province,China.Therefore,we investigated the anti-diabetic effects of HDB and its underlying mechanisms.We hypothesized that HDB tre...Objective:Huidouba(HDB) is a Chinese folk medicine used to treat diabetes in Sichuan Province,China.Therefore,we investigated the anti-diabetic effects of HDB and its underlying mechanisms.We hypothesized that HDB treatment could enhance glucose tolerance and insulin sensitivity,and thus prevent a hyperglycemia state.Methods:To test the hypothesis,streptozotocin(STZ)-induced diabetic mice and db/db mice,widely used models of hyperglycemia and insulin-resistant diabetes,were either treated with HDB,metformin,or acarbose.Blood glucose,oral glucose tolerance test,insulin tolerance test,pancreatic histopathology and serum biochemistry were detected to assess the hypoglycemic effect of HDB.Results:HDB treatments were found to show the effect in reducing glucose levels.HDB also resulted in a significant reduction in body weight and food intake in the STZ-induced diabetic mouse model.Furthermore,it significantly improved glucose and insulin tolerance in the two diabetic mouse models.Importantly,insulin,glucagon,pancreatic polypeptide,and somatostatin immunohistochemistry revealed that HDB treatment improved the function and the location of the cells in the islets compared with the other two treatments.HDB treatment resulted in significant restoration of islet function.Our results illustrated the underlying mechanism of HDB in the progression of diabetes,and HDB can be an effective agent for the treatment of diabetes.Conclusion:The results of this study suggested that HDB can reduce blood glucose levels in STZ-induced hyperglycemic mice and db/db mice.展开更多
基金Supported by the China Medical Foundation(No.20061108)
文摘The polysaccharide extracted from Huidouba（PEH） exihibited a significant anti-diabetic activity. The polysaccharide contained glucose, mannose and galactose in a content ratio of 1.00：0.34：0.32. After administration with 50-- 200 mg/kg body weight of PEH to type II diabetic mice induced by alloxan for four weeks, the blood glucose level of mice was decreased significantly. Moreover, the SOD activity increased at the same time（p〈0.01）. PEH showed a significant anti-diabetic and anti-oxidation activity. In addition, PEH affected the BUN and ALT levels（p〈0.05）, which are important characteristics of diabetes mellitus. Through this work, PEH should be a potential anti-diabetic drug in the future.
基金supported by the National Natural Science Foundation of China (No.81673957)State Key Laboratory of Natural and Biomimetic Drug (No.K20160210)+1 种基金Independent Research Projects for Ph.D.Students of Minzu University of China (No.181084)Undergraduate Research and Training Program (URTP) of Minzu University of China (No.GCCX2020110018)。
文摘Objective:Huidouba(HDB) is a Chinese folk medicine used to treat diabetes in Sichuan Province,China.Therefore,we investigated the anti-diabetic effects of HDB and its underlying mechanisms.We hypothesized that HDB treatment could enhance glucose tolerance and insulin sensitivity,and thus prevent a hyperglycemia state.Methods:To test the hypothesis,streptozotocin(STZ)-induced diabetic mice and db/db mice,widely used models of hyperglycemia and insulin-resistant diabetes,were either treated with HDB,metformin,or acarbose.Blood glucose,oral glucose tolerance test,insulin tolerance test,pancreatic histopathology and serum biochemistry were detected to assess the hypoglycemic effect of HDB.Results:HDB treatments were found to show the effect in reducing glucose levels.HDB also resulted in a significant reduction in body weight and food intake in the STZ-induced diabetic mouse model.Furthermore,it significantly improved glucose and insulin tolerance in the two diabetic mouse models.Importantly,insulin,glucagon,pancreatic polypeptide,and somatostatin immunohistochemistry revealed that HDB treatment improved the function and the location of the cells in the islets compared with the other two treatments.HDB treatment resulted in significant restoration of islet function.Our results illustrated the underlying mechanism of HDB in the progression of diabetes,and HDB can be an effective agent for the treatment of diabetes.Conclusion:The results of this study suggested that HDB can reduce blood glucose levels in STZ-induced hyperglycemic mice and db/db mice.
